Enhanced growth and quality of St. John's wort (Hypericum perforatum l.) under photoautotrophic in vitro conditions

2006 ◽  
Vol 42 (3) ◽  
pp. 278-282 ◽  
Author(s):  
M. A. Couceiro ◽  
F. Afreen ◽  
S. M. A. Zobayed ◽  
T. Kozai
Keyword(s):  
Hypericum Perforatum ◽  
St John’S Wort ◽  
St John's Wort ◽  
Hypericum Perforatum L

Quality and yield in St. John's wort (Hypericum perforatum L.) harvested in different phenological stages

2002 ◽  
Vol 50 (3) ◽  
pp. 349-358 ◽  
Author(s):  
F. Hevia ◽  
M. Berti ◽  
R. Wilckens
Keyword(s):  
Hypericum Perforatum ◽  
Block Design ◽  
Flower Bud ◽  
Phenological Stages ◽  
St John’S Wort ◽  
St John's Wort ◽  
Yield And Quality ◽  
Hypericum Perforatum L ◽  
Beginning Of Flowering

Two experiments were conducted in the province of Ñuble, Chile during the 1997/98 and 1998/99 seasons with the objective of evaluating the effect of harvesting date on the yield and quality of St. John's wort (Hypericum perforatum L.) in the second year of production. The apical 25 cm of the stem were harvested in the following stages: flower bud, beginning of flowering, full flower and petal drop. A randomized complete block design with four replications was used. The best yield (fresh, dry and threshing weight) and the highest hypericin content were obtained at the petal drop stage. Nevertheless, the results indicate that the best time to harvest St. John's wort is when 10 to 20% of the flowers are open and the rest are in the bud stage.

scholarly journals Development of a Micropropagation Protocol for St. John's Wort (Hypericum perforatum L.)

HortScience ◽  
2002 ◽  
Vol 37 (6) ◽  
pp. 978-980 ◽  
Author(s):  
Joe-Ann McCoy ◽  
N.D. Camper
Keyword(s):  
Depressive Disorders ◽  
Hypericum Perforatum ◽  
Leaf Disc ◽  
Multiple Shoots ◽  
Nodal Segments ◽  
St John’S Wort ◽  
St John's Wort ◽  
Explant Source ◽  
Hypericum Perforatum L

Hypericum perforatum L. (St. John's Wort) has an extensive history as an important medicinal herb used for the treatment of neurological and depressive disorders (Linde et al., 1996). The objective of this study was to establish an in vitro tissue culture protocol for St. John's Wort. Nodal segments, axillary buds, and leaf disc explants produced multiple shoots and callus on Murashige and Skoog minimal organics medium supplemented with combinations of indoleacetic acid (IAA; 0.57, 2.85, 5.71 μm) and benzylaminopurine (BA; 2.22, 4.44, 8.88 μm). Shoot production occurred on all combinations of IAA/BA tested and was significantly less in treatments without hormones. Callus production was higher on treatments containing 2.85 μm IAA + 4.44 μm BA, or 5.71 μm IAA + 8.88 μm BA. Shoots transferred to hormone-free medium at 8 weeks formed roots by 12 weeks. A micropropagation protocol was established for St. John's Wort using mature plants as the explant source.

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