Identification, Heterologous Expression and Biochemical Characterization of a Novel Cellulase-free Xylanase B from the Thermophilic Bacterium Cohnella sp.A01

2021 ◽  
Author(s):  
Hemad Rahimian Gavaseraei ◽  
Raheleh Hasanzadeh ◽  
Moslem Afsharnezhad ◽  
Ali Foroutan Kalurazi ◽  
S. Shirin Shahangian ◽  
...  
Keyword(s):  
Heterologous Expression ◽  
Biochemical Characterization ◽  
Thermophilic Bacterium

scholarly journals Heterologous expression in Pichia pastoris and biochemical characterization of the unmodified sulfatase from Fusarium proliferatum LE1

2017 ◽  
Vol 30 (8) ◽  
pp. 571-571 ◽  
Author(s):  
Svetlana A Korban ◽  
Kirill S Bobrov ◽  
Maria A Maynskova ◽  
Stanislav N Naryzhny ◽  
Olga L Vlasova ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Heterologous Expression ◽  
Biochemical Characterization ◽  
Fusarium Proliferatum

scholarly journals Heterologous Expression and Biochemical Characterization of Two Lipoxygenases in Oriental Melon, Cucumis melo var. makuwa Makino

PLoS ONE ◽  
2016 ◽  
Vol 11 (4) ◽  
pp. e0153801 ◽  
Author(s):  
Songxiao Cao ◽  
Hao Chen ◽  
Chong Zhang ◽  
Yufan Tang ◽  
Jieying Liu ◽  
...  
Keyword(s):  
Heterologous Expression ◽  
Cucumis Melo ◽  
Biochemical Characterization ◽  
Oriental Melon

scholarly journals Heterologous expression in Pichia pastoris and biochemical characterization of the unmodified sulfatase from Fusarium proliferatum LE1

2017 ◽  
Vol 30 (7) ◽  
pp. 477-488 ◽  
Author(s):  
Svetlana A. Korban ◽  
Kirill S. Bobrov ◽  
Maria A. Maynskova ◽  
Stanislav N. Naryzhny ◽  
Olga L. Vlasova ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Heterologous Expression ◽  
Biochemical Characterization ◽  
Fusarium Proliferatum

scholarly journals A Lysine Substitute for K+

2002 ◽  
Vol 277 (51) ◽  
pp. 49651-49654 ◽  
Author(s):  
Georgiy A. Belogurov ◽  
Reijo Lahti
Keyword(s):  
Escherichia Coli ◽  
Heterologous Expression ◽  
Phylogenetic Analyses ◽  
Functional Characterization ◽  
Membrane Vesicles ◽  
Thermophilic Bacterium ◽  
Inorganic Pyrophosphatase ◽  
Inner Membrane ◽  
Carboxydothermus Hydrogenoformans

The H+proton-translocating inorganic pyrophosphatase (H+-PPase) family is composed of two phylogenetically distinct types of enzymes: K+-dependent and K+-independent. However, to date, the sequence criteria governing this dichotomy have remained unknown. In this study, we describe the heterologous expression and functional characterization of H+-PPase from the thermophilic bacteriumCarboxydothermus hydrogenoformans. Both PPi-hydrolyzing and PPi-energized H+translocation activities of the recombinant enzyme inEscherichia coliinner membrane vesicles are strictly K+-dependent. Here we deduce the K+requirement of all available H+-PPase sequences based on the K+dependence ofC. hydrogenoformansH+-PPase in conjunction with phylogenetic analyses. Our data reveal that K+-independent H+-PPases possess conserved Lys and Thr that are absent in K+-dependent H+-PPases. We further demonstrate that a A460K substitution inC. hydrogenoformansH+-PPase is sufficient to confer K+independence to both PPihydrolysis and PPi-energized H+translocation. In contrast, a A463T mutation does not affect the K+dependence of H+-PPase.

Heterologous expression and biochemical characterization of a thermostable xylulose kinase fromBacillus coagulansIPE22

2019 ◽  
Vol 59 (5) ◽  
pp. 542-551
Author(s):  
Yuming Zhang ◽  
Chunhai Zhao ◽  
Zhihua Ni ◽  
Menghua Shao ◽  
Mengying Han ◽  
...  
Keyword(s):  
Heterologous Expression ◽  
Biochemical Characterization ◽  
Xylulose Kinase
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