Capacity for somatic embryogenesis of adult oil palm genitors (Elaeis guineensis, var. Pisifera) from immature leaf tissues

2020 ◽  
Vol 131 ◽  
pp. 229-239 ◽  
Author(s):  
Raphael Ferreira Almeida ◽  
Filipe Sathler Meira ◽  
Hugo Teixeira Gomes ◽  
Talita Aparecida Balzon ◽  
Patrícia Monah Cunha Bartos ◽  
...  
2018 ◽  
Vol 41 (4) ◽  
Author(s):  
Marlúcia Souza Pádua ◽  
Raíssa Silveira Santos ◽  
Luciano Vilela Paiva ◽  
Vanessa Cristina Stein ◽  
Luciano Coutinho Silva

ABSTRACT Oil palm is a woody monocot of economic importance due to high oil production from its fruits. Currently, the conventional method most used to propagate oil palm is seed germination, but success is limited by long time requirements and low germination percentage. An alternative for large-scale propagation of oil palm is the biotechnological technique of somatic embryogenesis. The rooting of plants germinated from somatic embryos is a difficult step, yet it is of great importance for later acclimatization and success in propagation. The aim of this study was to evaluate the effect of the auxins indole acetic acid (IAA) and indole butyric acid (IBA) on the rooting of somatic embryos of Tenera hybrid oil palm. Plants obtained by somatic embryogenesis were inoculated in modified MS medium with 10% sucrose and 0.6% agar and supplemented with IAA or IBA at concentrations of 5 µM, 10 µM, and 15 µM, and the absence of growth regulators. After 120 days, the presence of roots, root type, length of the longest root, number of roots, number of leaves, and shoot length were analyzed. Growth regulators were favorable to rooting; plants cultivated with IBA growth regulator at 15 µM showed higher rooting percentage (87%) and better results for the parameters of number of roots (1.33) and shoot length (9.83).


2021 ◽  
Vol 174 ◽  
pp. 114143
Author(s):  
Dapeng Zhang ◽  
Peng Shi ◽  
Yin M. Htwe ◽  
Zhiying Li ◽  
Leonard O. Ihase ◽  
...  

2014 ◽  
Vol 104 ◽  
pp. 112-127 ◽  
Author(s):  
Rafael de Carvalho Silva ◽  
Lilian Silveira Travassos Carmo ◽  
Zanderluce Gomes Luis ◽  
Luciano Paulino Silva ◽  
Jonny Everson Scherwinski-Pereira ◽  
...  

Plant Gene ◽  
2016 ◽  
Vol 8 ◽  
pp. 16-25 ◽  
Author(s):  
Siew-Eng Ooi ◽  
Zubaidah Ramli ◽  
Sharifah Shahrul Rabiah Syed Alwee ◽  
Harikrishna Kulaveerasingam ◽  
Meilina Ong-Abdullah

2019 ◽  
Vol 43 (1) ◽  
Author(s):  
Marlucia Souza Pádua Vilela ◽  
Jessica de Castro e Andrade ◽  
Raissa Silveira Santos ◽  
Vanessa Cristina Stein ◽  
Luciano Vilela Paiva

ABSTRACT Oil palm is economically important as a crop with high oil production. Indirect somatic embryogenesis in oil palm requires a long time for callus induction and plant formation, and it is important to study the embryogenic potential of calli and the mechanisms of somatic embryogenesis. The aim of this study was to test different growth regulators and spermine in induction of embryogenic calli in root explants of oil palm (Elaeis guineensis Jacq). Apex root explants of approximately 0.5 cm were isolated from plants cultivated in vitro and inoculated in Y3 culture medium in the following treatments: A - without growth regulators; B - 1 mg.L-1 picloram (4-amino-3,5,6-trichloro-2-pyridinecarboxylic acid); C - 1 mg.L-1 picloram and 2 mg.L-1 2ip (2-isopentenyladenine); D - 2 mg.L-1 2ip; E - 1 mg.L-1 picloram and 2 mg.L-1 BAP (6-benzylaminopurine); F - 2 mg.L-1 BAP; and G - 14.5 mg.L-1 spermine. After six months of culturing, the calli induced in the treatments were analyzed by light microscopy. The calli induced in the treatments with 1 mg.L-1picloram (B) and treatment with 14.5 mg.L-1spermine (G) exhibited embryogenic characteristics, small and isodiametric cells, forming agglomerates, besides a large amount of starch. Calli of the best treatment (Y3 com 1 mg.L-1 de picloram) were inoculated in Y3 culture medium without addition of growth regulators. After eight months, calli were once more analyzed under light microscopy. All the treatments showed callus formation, except for treatments D and A. Calli of treatment B exhibited cells with embryogenic characteristics that developed somatic embryos.


Sign in / Sign up

Export Citation Format

Share Document