proteomic identification
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Author(s):  
Vasiliki-Iris Perivolidi ◽  
Foteini Violitzi ◽  
Elisavet Ioannidou ◽  
Vagelis Rinotas ◽  
George Stamatakis ◽  
...  

2022 ◽  
Author(s):  
Smita Yadav ◽  
Sujin Byeon ◽  
Bailey Werner ◽  
Reilly Falter ◽  
Kristian Davidsen ◽  
...  

Septins are a family of cytoskeletal proteins that regulate several important aspects of neuronal development. Septin 7 (Sept7) is enriched at the base of dendritic spines in excitatory neurons and mediates both spine formation and spine-synapse maturation. Phosphorylation at a conserved C-terminal tail residue of Sept7 mediates its translocation into the dendritic spine head to allow spine-synapse maturation. The mechanistic basis for postsynaptic stability and compartmentalization conferred by phosphorylated Sept7, however, is not known. We report herein the proteomic identification of Sept7 phosphorylation dependent neuronal interactors. Using Sept7 C-terminal phosphopeptide pulldown and biochemical assays, we show that the 14-3-3 family of proteins specifically interact with Sept7 when phosphorylated at the T426 residue. Biochemically, we validate the interaction between Sept7 and 14-3-3 isoform gamma, and show that 14-3-3 gamma is also enriched in mature dendritic spine head. Further, we demonstrate that interaction of phosphorylated Sept7 with 14-3-3 protects it from dephosphorylation, as expression of a 14-3-3 antagonist significantly decreases phosphorylated Sept7 in neurons. This study identifies 14-3-3 proteins as an important physiological regulator of Sept7 function in neuronal development.


2021 ◽  
Vol 57 (6) ◽  
pp. 786-792
Author(s):  
L. I. Kovalev ◽  
M. A. Kovaleva ◽  
L. A. Novikova ◽  
I. M. Chernukha

Toxins ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 654
Author(s):  
Nicholas Kevin Willard ◽  
Emelyn Salazar ◽  
Fabiola Alejandra Oyervides ◽  
Cierra Siobhrie Wiebe ◽  
Jack Sutton Ocheltree ◽  
...  

The global exploration of snakebites requires the use of quantitative omics approaches to characterize snake venom as it enters into the systemic circulation. These omics approaches give insights into the venom proteome, but a further exploration is warranted to analyze the venom-reactome for the identification of snake venom biomarkers. The recent discovery of extracellular vesicles (EVs), and their critical cellular functions, has presented them as intriguing sources for biomarker discovery and disease diagnosis. Herein, we purified EV’s from the snake venom (svEVs) of Crotalus atrox and C. oreganus helleri, and from plasma of BALB/c mice injected with venom from each snake using EVtrap in conjunction with quantitative mass spectrometry for the proteomic identification and quantification of svEVs and plasma biomarkers. Snake venom EVs from C. atrox and C. o. helleri were highly enriched in 5′ nucleosidase, L-amino acid oxidase, and metalloproteinases. In mouse plasma EVs, a bioinformatic analysis for revealed upregulated responses involved with cytochrome P450, lipid metabolism, acute phase inflammation immune, and heat shock responses, while downregulated proteins were associated with mitochondrial electron transport, NADH, TCA, cortical cytoskeleton, reticulum stress, and oxidative reduction. Altogether, this analysis will provide direct evidence for svEVs composition and observation of the physiological changes of an envenomated organism.


Nitric Oxide ◽  
2021 ◽  
Author(s):  
Paschalis-Thomas Doulias ◽  
Margarita Tenopoulou ◽  
Iordanis Zakopoulos ◽  
Harry Ischiropoulos

Author(s):  
Karina P. De Sousa ◽  
Jeremy Potriquet ◽  
Jason Mulvenna ◽  
Javier Sotillo ◽  
Alex Loukas ◽  
...  

Molecules ◽  
2021 ◽  
Vol 26 (9) ◽  
pp. 2762
Author(s):  
Eliza Matuszewska ◽  
Joanna Matysiak ◽  
Grzegorz Rosiński ◽  
Elżbieta Kędzia ◽  
Weronika Ząbek ◽  
...  

Royal jelly (RJ) is a complex, creamy secretion produced by the glands of worker bees. Due to its health-promoting properties, it is used by humans as a dietary supplement. However, RJ compounds are not fully characterized yet. Hence, in this research, we aimed to broaden the knowledge of the proteomic composition of fresh RJ. Water extracts of the samples were pre-treated using combinatorial hexapeptide ligand libraries (ProteoMinerTM kit), trypsin-digested, and analyzed by a nanoLC-MALDI-TOF/TOF MS system. To check the ProteoMinerTM performance in the MS-based protein identification, we also examined RJ extracts that were not prepared with the ProteoMinerTM kit. We identified a total of 86 proteins taxonomically classified to Apis spp. (bees). Among them, 74 proteins were detected in RJ extracts pre-treated with ProteoMinerTM kit, and only 50 proteins were found in extracts non-enriched with this technique. Ten of the identified features were hypothetical proteins whose existence has been predicted, but any experimental evidence proves their in vivo expression. Additionally, we detected four uncharacterized proteins of unknown functions. The results of this research indicate that the ProteoMinerTM strategy improves proteomic identification in complex biological samples. Broadening the knowledge of RJ composition may contribute to the development of standards and regulations, enhancing the quality of RJ, and consequently, the safety of its supplementation.


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