scholarly journals HISTOLOGICAL ANALYSIS OF INDIRECT SOMATIC EMBRYOGENESIS INDUCED FROM ROOT EXPLATS OF OIL PALM (Elaeis guineensis Jacq)

2019 ◽  
Vol 43 (1) ◽  
Author(s):  
Marlucia Souza Pádua Vilela ◽  
Jessica de Castro e Andrade ◽  
Raissa Silveira Santos ◽  
Vanessa Cristina Stein ◽  
Luciano Vilela Paiva
Keyword(s):  
Somatic Embryogenesis ◽  
Light Microscopy ◽  
Growth Regulators ◽  
Oil Palm ◽  
Culture Medium ◽  
Elaeis Guineensis ◽  
Callus Formation ◽  
Root Explants ◽  
Indirect Somatic Embryogenesis ◽  
Elaeis Guineensis Jacq

ABSTRACT Oil palm is economically important as a crop with high oil production. Indirect somatic embryogenesis in oil palm requires a long time for callus induction and plant formation, and it is important to study the embryogenic potential of calli and the mechanisms of somatic embryogenesis. The aim of this study was to test different growth regulators and spermine in induction of embryogenic calli in root explants of oil palm (Elaeis guineensis Jacq). Apex root explants of approximately 0.5 cm were isolated from plants cultivated in vitro and inoculated in Y3 culture medium in the following treatments: A - without growth regulators; B - 1 mg.L-1 picloram (4-amino-3,5,6-trichloro-2-pyridinecarboxylic acid); C - 1 mg.L-1 picloram and 2 mg.L-1 2ip (2-isopentenyladenine); D - 2 mg.L-1 2ip; E - 1 mg.L-1 picloram and 2 mg.L-1 BAP (6-benzylaminopurine); F - 2 mg.L-1 BAP; and G - 14.5 mg.L-1 spermine. After six months of culturing, the calli induced in the treatments were analyzed by light microscopy. The calli induced in the treatments with 1 mg.L-1picloram (B) and treatment with 14.5 mg.L-1spermine (G) exhibited embryogenic characteristics, small and isodiametric cells, forming agglomerates, besides a large amount of starch. Calli of the best treatment (Y3 com 1 mg.L-1 de picloram) were inoculated in Y3 culture medium without addition of growth regulators. After eight months, calli were once more analyzed under light microscopy. All the treatments showed callus formation, except for treatments D and A. Calli of treatment B exhibited cells with embryogenic characteristics that developed somatic embryos.

scholarly journals Oil palm (Elaeis guineensis Jacq.) micropropagation via somatic embryogenesis from female inflorescences explants

2021 ◽  
Vol 912 (1) ◽  
pp. 012029
Author(s):  
N H Panggabean ◽  
M Basyuni ◽  
I Nurwahyuni ◽  
Elimasni
Keyword(s):  
Somatic Embryogenesis ◽  
Liquid Medium ◽  
Oil Palm ◽  
Embryogenic Callus ◽  
Elaeis Guineensis ◽  
Callus Formation ◽  
Basal Area ◽  
Regeneration Time ◽  
Elaeis Guineensis Jacq

Abstract The oil palm (Elaeis guineensis Jacq.) is a perennial woody oil crop in the Arecaceae family. Oil palm is well known for a long regeneration time, therefore, in vitro propagation received great enthusiasm from oil palm industries. Somatic embryogenesis (SE) has become one of the most promising clonal propagation techniques in recent times. This study was aimed to determine a protocol for micropropagation somatic embryogenesis from female inflorescences explants of oil palm. The explant used was obtained from the female inflorescences of the oil palm 2.5 years old Tenera variety. The basal media used was Y3 with the addition of the hormone 2,4-D with different concentrations (33,66,99 and 132 mg/L). Callus initiation was formed in 99 mg/L and 132 mg/L 2,4 – D concentration with the basal area and the percentage of callus formation is 31,25% and embryogenic callus was formed from primary callus development in the basal area of female florescence. Embryo somatic induction with cell suspension culture, because the liquid medium is more efficiently used in commercial-scale propagation. The embryogenic callus phase which is generally used in liquid medium is the nodular phase because it is still meristematic so that the potential for cell division is still high and can increase the percentage of embryogenic callus.

scholarly journals In vitro ROOTING OF TENERA HYBRID OIL PALM (Elaeis guineensis Jacq.) PLANTS1

2018 ◽  
Vol 41 (4) ◽  
Author(s):  
Marlúcia Souza Pádua ◽  
Raíssa Silveira Santos ◽  
Luciano Vilela Paiva ◽  
Vanessa Cristina Stein ◽  
Luciano Coutinho Silva
Keyword(s):  
Somatic Embryogenesis ◽  
Growth Regulators ◽  
Oil Palm ◽  
Somatic Embryos ◽  
Large Scale ◽  
Elaeis Guineensis ◽  
Germination Percentage ◽  
Shoot Length ◽  
Indole Butyric Acid

ABSTRACT Oil palm is a woody monocot of economic importance due to high oil production from its fruits. Currently, the conventional method most used to propagate oil palm is seed germination, but success is limited by long time requirements and low germination percentage. An alternative for large-scale propagation of oil palm is the biotechnological technique of somatic embryogenesis. The rooting of plants germinated from somatic embryos is a difficult step, yet it is of great importance for later acclimatization and success in propagation. The aim of this study was to evaluate the effect of the auxins indole acetic acid (IAA) and indole butyric acid (IBA) on the rooting of somatic embryos of Tenera hybrid oil palm. Plants obtained by somatic embryogenesis were inoculated in modified MS medium with 10% sucrose and 0.6% agar and supplemented with IAA or IBA at concentrations of 5 µM, 10 µM, and 15 µM, and the absence of growth regulators. After 120 days, the presence of roots, root type, length of the longest root, number of roots, number of leaves, and shoot length were analyzed. Growth regulators were favorable to rooting; plants cultivated with IBA growth regulator at 15 µM showed higher rooting percentage (87%) and better results for the parameters of number of roots (1.33) and shoot length (9.83).

Caffeate may play an important role in the somatic embryogenesis of oil palm (Elaeis guineensis Jacq.)

2021 ◽  
Vol 174 ◽  
pp. 114143
Author(s):  
Dapeng Zhang ◽  
Peng Shi ◽  
Yin M. Htwe ◽  
Zhiying Li ◽  
Leonard O. Ihase ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Oil Palm ◽  
Elaeis Guineensis ◽  
Elaeis Guineensis Jacq

Proteomic identification of differentially expressed proteins during the acquisition of somatic embryogenesis in oil palm (Elaeis guineensis Jacq.)

2014 ◽  
Vol 104 ◽  
pp. 112-127 ◽  
Author(s):  
Rafael de Carvalho Silva ◽  
Lilian Silveira Travassos Carmo ◽  
Zanderluce Gomes Luis ◽  
Luciano Paulino Silva ◽  
Jonny Everson Scherwinski-Pereira ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Oil Palm ◽  
Elaeis Guineensis ◽  
Differentially Expressed ◽  
Differentially Expressed Proteins ◽  
Proteomic Identification ◽  
Elaeis Guineensis Jacq

EgHOX1, a HD-Zip II gene, is highly expressed during early oil palm (Elaeis guineensis Jacq.) somatic embryogenesis

Plant Gene ◽  
2016 ◽  
Vol 8 ◽  
pp. 16-25 ◽  
Author(s):  
Siew-Eng Ooi ◽  
Zubaidah Ramli ◽  
Sharifah Shahrul Rabiah Syed Alwee ◽  
Harikrishna Kulaveerasingam ◽  
Meilina Ong-Abdullah
Keyword(s):  
Somatic Embryogenesis ◽  
Oil Palm ◽  
Elaeis Guineensis ◽  
Elaeis Guineensis Jacq

scholarly journals Effects of Growth Regulators and Type-Variety of Oil Palm (Elaeis guineensis Jacq.) on Direct Organogenesis

2018 ◽  
Vol 10 (2) ◽  
pp. 251-258
Author(s):  
Chibuzo Nwabueze Chikezie NWAOGUALA ◽  
Hakeem Olalekan SHITTU
Keyword(s):  
Growth Regulators ◽  
Oil Palm ◽  
Root Length ◽  
Elaeis Guineensis ◽  
Root Production ◽  
Ms Medium ◽  
Number Of Leaves ◽  
Leaves And Roots ◽  
Elaeis Guineensis Jacq

The in vitro organogenesis of embryos of type-variety (‘Albescens’, ‘Nigrescens’, ‘Virescens’ and ‘Idolatrica’) of the oil palm (Elaeis guineensis Jacq.) with growth regulators was studied. Murashige and Skoog (MS) medium, supplemented with nine treatment concentrations of Naphthalene acetic acid (NAA) and 6-Benzylaminopurine (BAP), alone and in combinations, was used to initiate embryo cultures of the type-variety. Data were collected on time of root production, root length, plant height, number of leaves and number of roots.  The results obtained indicated that there were swelling and expansion of the matured zygotic explants in cultures within 7 and 14 days after culture initiation (DCI). Time of root production did not vary significantly among type-variety, but differed significantly (p < 0.001) due to growth regulators, whereas their interaction effect was non-significant. Root length and plantlet height significantly varied (p < 0.05) due to type-variety and growth regulators, as well as their interaction. There were no significant variations among type-variety on number of leaves and roots produced at 84 DCI, but there was significant (p < 0.05) variations on number of leaves and non-significant variance on number of roots due to growth regulators. The type-variety combined with growth regulators effects on number of leaves and roots were not significant.  In the present study, the responses of the various type-variety in the regeneration of plantlets in vitro varied with respect to media supplementation and that low concentration of NAA and BAP, alone and in different combinations, favoured root, leaf and plantlet production in MS medium.

scholarly journals Callus and Somatic Embryogenesis from Cultured Anthers of Service Tree (Sorbus domestica L.)

HortScience ◽  
1995 ◽  
Vol 30 (5) ◽  
pp. 1078-1079 ◽  
Author(s):  
Isabel Arrillaga ◽  
Victoria Lerma ◽  
Pedro Pérez-Bermúdez ◽  
Juan Segura
Keyword(s):  
Somatic Embryogenesis ◽  
Growth Regulators ◽  
Culture Medium ◽  
Callus Formation ◽  
Microspore Embryogenesis ◽  
Microspore Development ◽  
Cold Pretreatment ◽  
Callus Proliferation ◽  
Indole 3 Acetic Acid ◽  
Embryogenic Response

The effects of stage of microspore development, cold pretreatment, and growth regulators on the embryogenic response of anthers from service tree (Sorbus domestica L.) are reported. Callus proliferation required the presence of growth regulators in the culture medium, best results being obtained with combinations of auxins and BA. Microspore embryogenesis was only induced when anthers containing tetrads or uninucleate pollen were cultured on BA-supplemented media containing IBA or IAA. A cold pretreatment before anther culture elicited a significant decrease in callus formation and inhibited embryogenesis. Chemical names used: benzyladenine (BA), indole-3-butyric acid (IBA), indole-3-acetic acid (IAA).

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