High sensitive single chain variable fragment screening from a microcystin-LR immunized mouse phage antibody library and its application in immunoassay

Talanta ◽  
2019 ◽  
Vol 197 ◽  
pp. 397-405 ◽  
Author(s):  
Chongxin Xu ◽  
Xiaoqin Liu ◽  
Yuan Liu ◽  
Xiao Zhang ◽  
Cunzheng Zhang ◽  
...  
Keyword(s):  
Single Chain Variable Fragment ◽  
Antibody Library ◽  
Single Chain ◽  
Phage Antibody ◽  
Fragment Screening ◽  
Phage Antibody Library

scholarly journals Clonning, Expression and Identification by Immunohistochemistry of Humanized Single-Chain Variable Fragment Antibody against Hepatitis C Virus Core Protein

2011 ◽  
Vol 60 (1) ◽  
pp. 13-17 ◽  
Author(s):  
YONG-ZHI LUN ◽  
JUN CHENG ◽  
YAN-WEI ZHONG ◽  
ZENG-GUO YU ◽  
QI WANG ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Core Protein ◽  
Microtiter Plate ◽  
Single Chain Variable Fragment ◽  
Antibody Library ◽  
Single Chain ◽  
Virus Core ◽  
Phage Antibody Library ◽  
Healthy Persons

Expression of single-chain variable fragment (scFv) antibodies on the surface of bacteriophage is widely used to prepare antibodies with pre-defined specificities. A phage antibody library containing the gene for scFv antibody against Hepatitis C virus core protein was panned with core protein immobilized on microtiter plate wells. After five rounds of panning 60 phage clones specific to core protein were obtained and one selected clone was sequenced. It was found that the specifically detected antigen consists of 774bp and is capable of encoding 257 amino acids in the patients but not in healthy persons.

In vitro and in vivo characterization of a human anti-c-erbB-2 single-chain Fv isolated from a filamentous phage antibody library

1995 ◽  
Vol 1 (1) ◽  
pp. 73-81 ◽  
Author(s):  
Robert Schier ◽  
James D. Marks ◽  
Ellen J. Wolf ◽  
Gerald Apelld ◽  
Cindy Won ◽  
...  
Keyword(s):  
Filamentous Phage ◽  
Antibody Library ◽  
Single Chain ◽  
Phage Antibody ◽  
Single Chain Fv ◽  
Phage Antibody Library ◽  
In Vivo Characterization
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