Genetic characterization of a mumps virus isolate during passaging in the amniotic cavity of embryonated chicken eggs

2004 ◽  
Vol 99 (2) ◽  
pp. 121-129 ◽  
Author(s):  
Jelena Ivancic ◽  
Dubravko Forcic ◽  
Tanja Kosutic Gulija ◽  
Renata Zgorelec ◽  
Leonida Repalust ◽  
...  
Keyword(s):  
Virus Isolate ◽  
Genetic Characterization ◽  
Mumps Virus ◽  
Amniotic Cavity ◽  
Chicken Eggs ◽  
Embryonated Chicken

scholarly journals Molecular characterization and phylogenetic analysis of orf virus isolated from goats in Sokoto metropolis, Nigeria

2021 ◽  
pp. FSO700
Author(s):  
Nafi'u Lawal ◽  
Mubarak Ibrahim ◽  
Dauda Ayomide Onawala ◽  
Muhammad Bashir Bello ◽  
Rabiu Muhammad Aliyu ◽  
...  
Keyword(s):  
Genetic Characterization ◽  
Orf Virus ◽  
Coding Region ◽  
B2l Gene ◽  
Polymerase Chain ◽  
Chicken Eggs ◽  
Unique Amino Acid ◽  
Close Genetic Relationship ◽  
Embryonated Chicken

Aim: The aim of this study was to molecularly characterize orf virus isolated from clinical infections in goats in Sokoto metropolis. Materials & methods: Embryonated chicken eggs were used to isolate orf virus according to the established protocol. Viral DNA was extracted and full coding region of B2L gene was amplified by polymerase chain reaction, sequenced and blasted for identification and phylogenetically analyzed. Results and discussion: The B2L gene sequences of the isolate showed slight variability (96–98.7%) with the reference sequences as it clustered within the same clade with Korean, Zambian and Ethiopian strains, signifying a close genetic relationship. Unique amino acid substitutions were noted. This is the first genetic characterization of B2L gene of orf virus circulating in Nigeria. Conclusion: This study has provided in sight into the genetic diversity of orf virus in the study area.

scholarly journals Recovery and Genetic Characterization of a West Nile Virus Isolate from China

2020 ◽  
Author(s):  
Yan Guo ◽  
Hongjiang Wang ◽  
Songtao Xu ◽  
Hangyu Zhou ◽  
Chao Zhou ◽  
...  
Keyword(s):  
West Nile Virus ◽  
Virus Isolate ◽  
Genetic Characterization ◽  
West Nile

Genetic characterization of a border disease virus isolate originating from Slovakia

2013 ◽  
Vol 57 (01) ◽  
pp. 17-25 ◽  
Author(s):  
V. LEŠKOVÁ ◽  
A. JACKOVÁ ◽  
M. VLASÁKOVÁ ◽  
S. VILČEK
Keyword(s):  
Disease Virus ◽  
Virus Isolate ◽  
Genetic Characterization ◽  
Border Disease Virus ◽  
Border Disease

scholarly journals Seroprevalence of measles, mumps, and rubella and genetic characterization of mumps virus in Khartoum, Sudan

2020 ◽  
Vol 91 ◽  
pp. 87-93
Author(s):  
Omer Adam ◽  
Ahmed Musa ◽  
Amani Kamer ◽  
Aurélie Sausy ◽  
Elsa Tisserand ◽  
...  
Keyword(s):  
Genetic Characterization ◽  
Mumps Virus

Antigenic and genetic characterization of the fusion (F) protein of mumps virus strains

2000 ◽  
Vol 145 (6) ◽  
pp. 1199-1210 ◽  
Author(s):  
T. Tecle ◽  
B. Johansson ◽  
Z. Yun ◽  
C. Örvell
Keyword(s):  
Genetic Characterization ◽  
Mumps Virus ◽  
F Protein ◽  
Virus Strains

scholarly journals Molecular Characterization and Phylogenetic Analysis of Orf Virus Isolated From Goats in Sokoto Metropolis, Nigeria

2020 ◽  
Author(s):  
Nafiu Lawal
Keyword(s):  
Molecular Characterization ◽  
Disease Outbreaks ◽  
Coding Region ◽  
Korean Strain ◽  
B2l Gene ◽  
Chicken Eggs ◽  
Unique Amino Acid ◽  
Close Genetic Relationship ◽  
Embryonated Chicken

Aim: Despite the endemic nature of contagious ecthyma in Nigeria, there is limited report on the molecular characterization of the isolates responsible for disease outbreaks. The aim of this study was to molecularly characterize ORFV isolated from clinical infections in goats in Sokoto metropolis. Materials and Methods: Seronegative embryonated chicken eggs were used to isolate ORFV via the chorio allantoic membrane (CAM) route according to the established protocol. Viral DNA was extracted from infected CAM and the full coding region of B2L gene was amplified by PCR and subsequently sequenced by Sanger’s method. The nucleotide sequence results were blasted for identification and phylogenetically analyzed using MEGA and Bioedit softwares. Results and Discussion: The results showed that B2L gene sequences of the ORFV UDUS/01/19/More strain showed slight variability (96- 98.7%) with the reference sequences. Our isolate clustered within the same clade with Korean strain signifying a close genetic relationship. Unique amino acid substitutions were noted in our isolate when compared with other references. This is arguably the first genetic characterisation of B2L gene of ORFV circulating in Nigeria. Conclusion: Our study has provided in sight into the genetic diversity of ORFV in the study area. This is crucial for the design of effective vaccines against the disease which are currently lacking in the country.

ISOLATION AND CHARACTERIZATION OF INFECTIOUS LARYNGOTRACHEITIS VIRUS IN LAYER CHICKENS

2012 ◽  
Vol 8 (2) ◽  
pp. 123-130 ◽  
Author(s):  
MS Islam ◽  
MSR Khan ◽  
MA Islam ◽  
J Hassan ◽  
S Affroze ◽  
...  
Keyword(s):  
Neutralization Test ◽  
Passive Haemagglutination ◽  
Isolation And Characterization ◽  
Infectious Laryngotracheitis ◽  
Commercial Farms ◽  
Layer Chickens ◽  
Chicken Eggs ◽  
Virus Isolates ◽  
Embryonated Chicken

The present research work was conducted for the isolation and characterization of infectious laryngotracheitis (ILT) virus in layer chickens from commercial farms of Gazipur District. A total of 25 field samples were collected from suspected layer chickens of five commercial farms and were cultivated into 10-12 days old embryonated chicken eggs through chorioallantoic membrane (CAM) route for isolation of field virus. The field viruses were characterized by physico-chemical properties against pH, heat, ether and chloroform, serological test such as virus neutralization test (VNT) and passive haemagglutination (PHA) test and pathogenicity testing. In the embryonated chicken eggs, virus produced discrete pock lesions as early as 2 days of post inoculation and embryo death was recorded within 4-6 days of inoculation. The viruses could be inactivated by pH 4 within 2 hours. Inactivation of viruses was observed at 600C for 6 minutes, 550C for 15 minutes and 380C for 2 days. Ether-chloroform treatment also inactivated the viruses. Virus neutralization test revealed that all the virus isolates were neutralized by antiserum to ILT vaccine. Passive haemagglutination test showed that the tanned sheep RBC sensitized with the virus isolates were agglutinated in presence of the antiserum to ILT vaccine. The pathogenicity test recorded 100% mortality in experimental chickens. Data of this study suggest that the field isolates might be infectious laryngotracheitis virus.DOI = http://dx.doi.org/10.3329/bjvm.v8i2.11194 Bangl. J. Vet. Med. (2010). 8 (2) : 123-130 

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