ISOLATION AND CHARACTERIZATION OF INFECTIOUS LARYNGOTRACHEITIS VIRUS IN LAYER CHICKENS

2012 ◽  
Vol 8 (2) ◽  
pp. 123-130 ◽  
Author(s):  
MS Islam ◽  
MSR Khan ◽  
MA Islam ◽  
J Hassan ◽  
S Affroze ◽  
...  
Keyword(s):  
Neutralization Test ◽  
Passive Haemagglutination ◽  
Isolation And Characterization ◽  
Infectious Laryngotracheitis ◽  
Commercial Farms ◽  
Layer Chickens ◽  
Chicken Eggs ◽  
Virus Isolates ◽  
Embryonated Chicken

The present research work was conducted for the isolation and characterization of infectious laryngotracheitis (ILT) virus in layer chickens from commercial farms of Gazipur District. A total of 25 field samples were collected from suspected layer chickens of five commercial farms and were cultivated into 10-12 days old embryonated chicken eggs through chorioallantoic membrane (CAM) route for isolation of field virus. The field viruses were characterized by physico-chemical properties against pH, heat, ether and chloroform, serological test such as virus neutralization test (VNT) and passive haemagglutination (PHA) test and pathogenicity testing. In the embryonated chicken eggs, virus produced discrete pock lesions as early as 2 days of post inoculation and embryo death was recorded within 4-6 days of inoculation. The viruses could be inactivated by pH 4 within 2 hours. Inactivation of viruses was observed at 600C for 6 minutes, 550C for 15 minutes and 380C for 2 days. Ether-chloroform treatment also inactivated the viruses. Virus neutralization test revealed that all the virus isolates were neutralized by antiserum to ILT vaccine. Passive haemagglutination test showed that the tanned sheep RBC sensitized with the virus isolates were agglutinated in presence of the antiserum to ILT vaccine. The pathogenicity test recorded 100% mortality in experimental chickens. Data of this study suggest that the field isolates might be infectious laryngotracheitis virus.DOI = http://dx.doi.org/10.3329/bjvm.v8i2.11194 Bangl. J. Vet. Med. (2010). 8 (2) : 123-130 

Presence, isolation and characterization of yolk DNA from chicken eggs

1998 ◽  
Vol 41 (3) ◽  
pp. 251-257 ◽  
Author(s):  
Lijun Sun ◽  
Lei Yu ◽  
Chuchu Chen
Keyword(s):  
Isolation And Characterization ◽  
Chicken Eggs ◽  
Yolk Dna

scholarly journals Isolation and characterization of H9N2 influenza virus isolates from poultry respiratory disease outbreak

SpringerPlus ◽  
2014 ◽  
Vol 3 (1) ◽  
Author(s):  
Subhash J Jakhesara ◽  
Vaibhav D Bhatt ◽  
Namrata V Patel ◽  
Kantilal S Prajapati ◽  
Chaitanya G Joshi
Keyword(s):  
Influenza Virus ◽  
Respiratory Disease ◽  
Disease Outbreak ◽  
H9n2 Influenza Virus ◽  
Isolation And Characterization ◽  
Virus Isolates

Genetic characterization of a mumps virus isolate during passaging in the amniotic cavity of embryonated chicken eggs

2004 ◽  
Vol 99 (2) ◽  
pp. 121-129 ◽  
Author(s):  
Jelena Ivancic ◽  
Dubravko Forcic ◽  
Tanja Kosutic Gulija ◽  
Renata Zgorelec ◽  
Leonida Repalust ◽  
...  
Keyword(s):  
Virus Isolate ◽  
Genetic Characterization ◽  
Mumps Virus ◽  
Amniotic Cavity ◽  
Chicken Eggs ◽  
Embryonated Chicken

Molecular characterization of infectious laryngotracheitis virus in naturally infected egg layer chickens in a multi-age flock in Brazil

2014 ◽  
Vol 160 (1) ◽  
pp. 241-252 ◽  
Author(s):  
Rodrigo de Macedo Couto ◽  
Ingred Sales Preis ◽  
Juliana Fortes Vilarinho Braga ◽  
Bruno S. A. F. Brasil ◽  
Marcela Gonçalves Drummond ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Infectious Laryngotracheitis Virus ◽  
Infectious Laryngotracheitis ◽  
Layer Chickens ◽  
Laryngotracheitis Virus

Isolation and Characterization of Monoclonal Antibodies Against Structural Proteins of Infectious Laryngotracheitis Virus

2003 ◽  
Vol 47 (2) ◽  
pp. 330-342 ◽  
Author(s):  
Jutta Veits ◽  
Bernd Köllner ◽  
Jens P. Teifke ◽  
Harald Granzow ◽  
Thomas C. Mettenleiter ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Structural Proteins ◽  
Infectious Laryngotracheitis Virus ◽  
Isolation And Characterization ◽  
Infectious Laryngotracheitis ◽  
Laryngotracheitis Virus

scholarly journals Teratogenicity of the Palyam serogroup orbiviruses in the embryonated chicken egg model

1991 ◽  
Vol 106 (1) ◽  
pp. 179-188 ◽  
Author(s):  
T. Whistler ◽  
R. Swanepoel
Keyword(s):  
Statistical Analysis ◽  
Yolk Sac ◽  
Serological Tests ◽  
Pathogenic Potential ◽  
Chicken Egg ◽  
Congenital Deformities ◽  
Teratogenic Potential ◽  
Chicken Eggs ◽  
Virus Isolates ◽  
Embryonated Chicken

SUMMARYEmbryonated chicken eggs were used as a model for assessing the teratogenic potential of several Palyam serogroup orbiviruses. Infection of 4-day-old embryonated chicken eggs via the yolk sac with eight of the viruses resulted in deaths or congenital deformities which included retarded development, arthrogryposis and reduced feathering. Statistical analysis showed that the viruses could be divided into three groups: those that caused death (Gweru virus isolates 866/77 and 1726/7776 and Apies River virus), those that caused deaths only when large amounts of virus were inoculated (Gweru isolate AR11869 and Marondera virus) and those that caused death and deformities (Abadina, Kasba, Nyabira, Petevo and Vellore viruses). Differences in pathogenic potential were noted between isolates identified as the same serotype by serological tests.

scholarly journals Isolation and characterization of infectious Vibrio parahaemolyticus, the causative agent of AHPND, from the whiteleg shrimp (Litopenaeus vannamei)

2017 ◽  
Vol 44 (3) ◽  
pp. 470-479
Author(s):  
Patricia López-León ◽  
Antonio Luna-González ◽  
Ruth Escamilla-Montes ◽  
María del Carmen Flores-Miranda ◽  
Jesús A. Fierro-Coronado ◽  
...  
Keyword(s):  
Causative Agent ◽  
Biofilm Formation ◽  
Vibrio Parahaemolyticus ◽  
Bacterial Strains ◽  
Isolation And Characterization ◽  
Commercial Farms ◽  
Whiteleg Shrimp ◽  
Acute Hepatopancreatic Necrosis Disease ◽  
Selection Of

Vibrio parahaemolyticus, the causative agent of acute hepatopancreatic necrosis disease (AHPND), was isolated from the hepatopancreas of moribund whiteleg shrimp of commercial farms from Guasave, Sinaloa, Mexico. The isolates were screened on thiosulfate citrate bile salt sucrose agar plates for the selection of green colonies and further characterized through PCR with AP3 primers, 89F/R primers, hemolysin genes, hemolytic and enzymatic activity, hydrophobicity, autoaggregation, and biofilm formation. Bioassays by immersion challenge were conducted to confirm the pathogenicity of selected bacterial strains. In addition, the LC50 was calculated for each isolate. All isolates (35) belonged to V. parahaemolyticus, but three isolates did not correspond to strains that cause AHPND since they were negative with 89F/R primers. All isolates were αhemolytic and showed biofilm formation (from moderate to strong). Isolates were hydrophobic or hydrophilic and showed high autoaggregation capacity. Eight strains did not kill shrimp and eleven were pathogenic, but differences in virulence were found among them perhaps due to α-hemolysis and differences in biofilm formation and hydrophobicity. Therefore, performed characterization may help to understand the pathogenicity of V. parahaemolyticus. Finally, results showed that smaller shrimp are less resistant to V. parahaemolyticus infection.

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