scholarly journals Challenges, pitfalls and surprises: development and validation of a monoclonal antibody for enzyme immunoassay of the steroid 1α-hydroxycorticosterone in elasmobranch species

2018 ◽  
Vol 265 ◽  
pp. 83-89 ◽  
Author(s):  
Catharine J. Wheaton ◽  
Natalie D. Mylniczenko ◽  
John M. Rimoldi ◽  
Rama S.V.S. Gadepalli ◽  
R. Hart ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Enzyme Immunoassay ◽  
Elasmobranch Species ◽  
Development And Validation

Development and validation of a monoclonal antibody enzyme immunoassay for measuring progesterone in saliva

1994 ◽  
Vol 40 (3) ◽  
pp. 454-458 ◽  
Author(s):  
A O'Rorke ◽  
M M Kane ◽  
J P Gosling ◽  
D F Tallon ◽  
P F Fottrell
Keyword(s):  
Monoclonal Antibody ◽  
Enzyme Immunoassay ◽  
Solid Phase ◽  
Saliva Sample ◽  
Extraction Step ◽  
Lower Detection ◽  
Bovine Serum Albumin Conjugate ◽  
Normal Women ◽  
Development And Validation ◽  
Peroxidase Conjugate

Abstract A nonextraction, competitive, solid-phase enzyme immunoassay with a monoclonal antibody was developed and validated for measuring progesterone in saliva. The antibody was raised against 11 alpha-hydroxyprogesterone hemisuccinate-bovine serum albumin conjugate and was indirectly immobilized to the walls of microtiter wells. The labeled analyte (progesterone-horseradish peroxidase conjugate) was homologous with the immunogen. The lower detection limit (concentration equivalent to B0-3 SD) was 38 pmol/L of saliva sample. We validated the assay with studies to establish the independence of the concentration determined from the volume of saliva assayed, quantitative recovery of progesterone added to saliva, interference from possible cross-reactants, and agreement with a similar assay that incorporated an extraction step. In addition, we determined luteal-phase concentrations of salivary progesterone in normal women and compared the results with those of an older assay involving a polyclonal antibody.

Development of a Monoclonal Antibody-Based Enzyme Immunoassay for the Pyrethroid Insecticide Deltamethrin

2010 ◽  
Vol 58 (14) ◽  
pp. 8189-8195 ◽  
Author(s):  
Ye Kong ◽  
Qi Zhang ◽  
Wen Zhang ◽  
Shirley J. Gee ◽  
Peiwu Li
Keyword(s):  
Monoclonal Antibody ◽  
Enzyme Immunoassay ◽  
Pyrethroid Insecticide

scholarly journals Bound/free separation methods in steroid enzyme immunoassay with monoclonal antibody.

1988 ◽  
Vol 36 (9) ◽  
pp. 3525-3531 ◽  
Author(s):  
HIROSHI HOSODA ◽  
REIKO TSUKAMOTO ◽  
SAKIKO TAMURA ◽  
TOSHIO NAMBARA
Keyword(s):  
Monoclonal Antibody ◽  
Enzyme Immunoassay

Development and Validation of an Ultrasensitive Chemiluminescent Enzyme Immunoassay for Aflatoxin M1in Milk

2005 ◽  
Vol 53 (9) ◽  
pp. 3300-3305 ◽  
Author(s):  
Maria Magliulo ◽  
Mara Mirasoli ◽  
Patrizia Simoni ◽  
Rosella Lelli ◽  
Ottavio Portanti ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
Development And Validation ◽  
Chemiluminescent Enzyme Immunoassay

Development and validation of an indirect enzyme immunoassay for detection of ovine antibody to Brucella ovis

1997 ◽  
Vol 54 (3-4) ◽  
pp. 357-368 ◽  
Author(s):  
Ana M. Vigliocco ◽  
Patricia S. Silva Paulo ◽  
Joaquín Mestre ◽  
Gabriel C. Briones ◽  
Graciela Draghi ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
Brucella Ovis ◽  
Development And Validation

scholarly journals An enzyme immunoassay system with a monoclonal antibody for the determination of 11-deoxycortisol.

1987 ◽  
Vol 35 (4) ◽  
pp. 1497-1502 ◽  
Author(s):  
HIROSHI HOSODA ◽  
SAKIKO TAMURA ◽  
REIKO TSUKAMOTO ◽  
NORIHIRO KOBAYASHI ◽  
JUN-ICHI SAWADA ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Enzyme Immunoassay
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