Noninvasive Fetal Sex Determination Using Cell-Free Fetal DNA: A Systematic Review and Meta-analysis

2013 ◽  
Vol 2013 ◽  
pp. 437-439
Author(s):  
J.A. Stockman
Keyword(s):  
Systematic Review ◽  
Sex Determination ◽  
Meta Analysis ◽  
Fetal Sex ◽  
Fetal Dna ◽  
Cell Free Fetal Dna

Noninvasive Fetal Sex Determination Using Cell-Free Fetal DNA: A Systematic Review and Meta-Analysis

2011 ◽  
Vol 66 (12) ◽  
pp. 741-742
Author(s):  
Stephanie A. Devaney ◽  
Glenn E. Palomaki ◽  
Joan A. Scott ◽  
Diana W. Bianchi
Keyword(s):  
Systematic Review ◽  
Sex Determination ◽  
Meta Analysis ◽  
Fetal Sex ◽  
Fetal Dna ◽  
Cell Free Fetal Dna

Noninvasive fetal sex determination by analysis of cell-free fetal DNA in maternal capillary blood obtained by fingertip puncture

2018 ◽  
Vol 38 (8) ◽  
pp. 620-623
Author(s):  
Ticiane Henriques Santa Rita ◽  
Camila Santos Nobre ◽  
Rafael Henriques Jácomo ◽  
Lídia Freire Abdalla Nery ◽  
Gustavo Barcelos Barra
Keyword(s):  
Sex Determination ◽  
Capillary Blood ◽  
Fetal Sex ◽  
Fetal Dna ◽  
Cell Free Fetal Dna

scholarly journals Ovine fetal sex determination using circulating cell-free fetal DNA (ccffDNA) and cervical mucous secretions

2015 ◽  
Vol 4 (1) ◽  
pp. 65-69 ◽  
Author(s):  
R Asadpour ◽  
MH Asadi ◽  
R Jafari-Joozani ◽  
GH Hamidian
Keyword(s):  
Sex Determination ◽  
Fetal Sex ◽  
Fetal Dna ◽  
Cell Free Fetal Dna ◽  
Ovine Fetal

scholarly journals Fetal Sex Determination Using Cell-Free Fetal Dna (cffDNA) in Maternal Blood

2016 ◽  
Vol 5 (2) ◽  
pp. 89 ◽  
Author(s):  
I Nyoman Hariyasa Sanjaya ◽  
Tjok Gde Agung Suwardewa ◽  
I G Kamasan N. Arijana
Keyword(s):  
Sex Determination ◽  
Maternal Blood ◽  
Fetal Sex ◽  
Fetal Dna ◽  
Cell Free Fetal Dna

Fetal sex determination in twin pregnancies using cell free fetal DNA analysis

2018 ◽  
Vol 38 (8) ◽  
pp. 578-584 ◽  
Author(s):  
Miguel Milan ◽  
Emilia Mateu ◽  
David Blesa ◽  
Monica Clemente-Ciscar ◽  
Carlos Simon
Keyword(s):  
Sex Determination ◽  
Dna Analysis ◽  
Fetal Sex ◽  
Fetal Dna ◽  
Cell Free Fetal Dna ◽  
Twin Pregnancies

scholarly journals Noninvasive Fetal Sex Determination Using Cell-Free Fetal DNA

JAMA ◽  
2011 ◽  
Vol 306 (6) ◽  
Author(s):  
Stephanie A. Devaney ◽  
Glenn E. Palomaki ◽  
Joan A. Scott ◽  
Diana W. Bianchi
Keyword(s):  
Sex Determination ◽  
Fetal Sex ◽  
Fetal Dna ◽  
Cell Free Fetal Dna

scholarly journals Evaluation of Sample Stability and Automated DNA Extraction for Fetal Sex Determination Using Cell-Free Fetal DNA in Maternal Plasma

2013 ◽  
Vol 2013 ◽  
pp. 1-4 ◽  
Author(s):  
Elena Ordoñez ◽  
Laura Rueda ◽  
M. Paz Cañadas ◽  
Carme Fuster ◽  
Vincenzo Cirigliano
Keyword(s):  
Sex Determination ◽  
Dna Extraction ◽  
Maternal Plasma ◽  
Daily Routine ◽  
Reference Laboratory ◽  
Fetal Sex ◽  
Fetal Dna ◽  
Sample Stability ◽  
Cell Free Fetal Dna ◽  
Automated Dna Extraction

Objective. The detection of paternally inherited sequences in maternal plasma, such as the SRY gene for fetal sexing or RHD for fetal blood group genotyping, is becoming part of daily routine in diagnostic laboratories. Due to the low percentage of fetal DNA, it is crucial to ensure sample stability and the efficiency of DNA extraction. We evaluated blood stability at 4°C for at least 24 hours and automated DNA extraction, for fetal sex determination in maternal plasma.Methods. A total of 158 blood samples were collected, using EDTA-K tubes, from women in their 1st trimester of pregnancy. Samples were kept at 4°C for at least 24 hours before processing. An automated DNA extraction was evaluated, and its efficiency was compared with a standard manual procedure. The SRY marker was used to quantify cfDNA by real-time PCR.Results. Although lower cfDNA amounts were obtained by automated DNA extraction (mean 107,35 GE/mL versus 259,43 GE/mL), the SRY sequence was successfully detected in all 108 samples from pregnancies with male fetuses.Conclusion. We successfully evaluated the suitability of standard blood tubes for the collection of maternal blood and assessed samples to be suitable for analysis at least 24 hours later. This would allow shipping to a central reference laboratory almost from anywhere in Europe.

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