A complex chromosome rearrangement involving chromosome 8, 11, and 12 analyzed by conventional cytogenetic investigations, fluorescence in situ hybridisation, and spectral karyotyping

2001 ◽  
Vol 44 (3) ◽  
pp. 135-138 ◽  
Author(s):  
D Kotzot ◽  
H Holland ◽  
M Köhler ◽  
U.G Froster
Keyword(s):  
Chromosome Rearrangement ◽  
In Situ Hybridisation ◽  
Chromosome 8 ◽  
Fluorescence In Situ Hybridisation ◽  
Spectral Karyotyping ◽  
Complex Chromosome Rearrangement ◽  
Complex Chromosome

scholarly journals Familial complex chromosome rearrangement ascertained by in situ hybridisation.

1997 ◽  
Vol 34 (2) ◽  
pp. 164-166 ◽  
Author(s):  
C Fuster ◽  
L Miguez ◽  
R Miro ◽  
M A Rigola ◽  
A Perez ◽  
...  
Keyword(s):  
Chromosome Rearrangement ◽  
In Situ Hybridisation ◽  
Complex Chromosome Rearrangement ◽  
Complex Chromosome

Aneuploidy of chromosome 8 detected by fluorescence in situ hybridisation in ACP01 cell line gastric adenocarcinoma

2006 ◽  
Vol 6 (3) ◽  
pp. 129-133 ◽  
Author(s):  
A. Costa Guimarães ◽  
L. Gonçalves Quintana ◽  
M. Ferreira Leal ◽  
S. Satomi Takeno ◽  
P. Pimentel Assumpção ◽  
...  
Keyword(s):  
Cell Line ◽  
Gastric Adenocarcinoma ◽  
In Situ Hybridisation ◽  
Chromosome 8 ◽  
Fluorescence In Situ Hybridisation

scholarly journals Rapid Multi-Hybridisation FISH Screening for Balanced Porcine Reciprocal Translocations Suggests a Much Higher Abnormality Rate Than Previously Appreciated

Cells ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 250
Author(s):  
Rebecca E O’Connor ◽  
Lucas G Kiazim ◽  
Claudia C Rathje ◽  
Rebecca L Jennings ◽  
Darren K Griffin
Keyword(s):  
Semen Analysis ◽  
In Situ Hybridisation ◽  
Economic Loss ◽  
Environmental Damage ◽  
Fluorescence In Situ Hybridisation ◽  
Reciprocal Translocations ◽  
Chromosome Translocations ◽  
Farrowing Rate ◽  
Significant Economic Loss

With demand rising, pigs are the world’s leading source of meat protein; however significant economic loss and environmental damage can be incurred if boars used for artificial insemination (AI) are hypoprolific (sub-fertile). Growing evidence suggests that semen analysis is an unreliable tool for diagnosing hypoprolificacy, with litter size and farrowing rate being more applicable. Once such data are available, however, any affected boar will have been in service for some time, with significant financial and environmental losses incurred. Reciprocal translocations (RTs) are the leading cause of porcine hypoprolificacy, reportedly present in 0.47% of AI boars. Traditional standard karyotyping, however, relies on animal specific expertise and does not detect more subtle (cryptic) translocations. Previously, we reported development of a multiple hybridisation fluorescence in situ hybridisation (FISH) strategy; here, we report on its use in 1641 AI boars. A total of 15 different RTs were identified in 69 boars, with four further animals XX/XY chimeric. Therefore, 4.5% had a chromosome abnormality (4.2% with an RT), a 0.88% incidence. Revisiting cases with both karyotype and FISH information, we reanalysed captured images, asking whether the translocation was detectable by karyotyping alone. The results suggest that chromosome translocations in boars may be significantly under-reported, thereby highlighting the need for pre-emptive screening by this method before a boar enters a breeding programme.

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