Active transport inhibition in rat small intestine by amphiphilic amines: an In Vitro study with various local anaesthetics

2000 ◽  
Vol 59 (8) ◽  
pp. 907-913 ◽  
Author(s):  
Gerhard J Strugala ◽  
Bernd Elsenhans ◽  
Wolfgang Forth
Keyword(s):  
Small Intestine ◽  
Active Transport ◽  
In Vitro Study ◽  
Local Anaesthetics ◽  
Vitro Study ◽  
Rat Small Intestine ◽  
Transport Inhibition

PepT1-mediated fMLP transport induces intestinal inflammation in vivo

2002 ◽  
Vol 283 (6) ◽  
pp. C1795-C1800 ◽  
Author(s):  
Marion Buyse ◽  
Annick Tsocas ◽  
Francine Walker ◽  
Didier Merlin ◽  
Andre Bado
Keyword(s):  
Small Intestine ◽  
Histological Analysis ◽  
Intestinal Inflammation ◽  
In Vitro Study ◽  
Peptide Transporter ◽  
Rat Colon ◽  
Membrane Transporter ◽  
Rat Small Intestine

In the present study, the effect of H+/peptide transporter (PepT1)-mediated N-formylmethionyl-leucyl-phenylalanine (fMLP) transport on inflammation in vivo in the rat small intestine, which expresses high PepT1 levels, and in the rat colon, which does not express PepT1, were investigated using myeloperoxidase (MPO) activity and histological analysis. We found that 10 μM fMLP perfusion in the jejunum for 4 h significantly increased MPO activity and altered the architecture of jejunal villi. In contrast, 10 μM fMLP perfusion in the colon for 4 h did not induce any inflammation. In addition, we have shown that 50 mM Gly-Gly alone did not affect basal MPO activity but completely inhibited the MPO activity induced by 10 μM fMLP in the jejunum. Together, these experiments demonstrate that 1) the differential expression of PepT1 between the small intestine and the colon plays an important role in epithelial-neutrophil interactions and 2) the inhibition of fMLP uptake by jejunal epithelial cells (expressing PepT1) reduces the neutrophil ability to move across the epithelium, in agreement with our previously published in vitro study. This report constitutes the first in vivo study showing the implication of a membrane transporter (PepT1) in intestinal inflammation.

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