scholarly journals Effect of furosemide on vascular permeability in passive cutaneous anaphylaxis response in the rat skin and on constriction of isolated guinea-pig trachea induced with chemical mediators

1992 ◽  
Vol 58 ◽  
pp. 295
Author(s):  
Isamu Komine ◽  
Noriko Horigome ◽  
Yukio Amano ◽  
Masafumi Yaguchi ◽  
Kazuo Sugio ◽  
...  
Keyword(s):  
Guinea Pig ◽  
Vascular Permeability ◽  
Passive Cutaneous Anaphylaxis ◽  
Rat Skin ◽  
Chemical Mediators

The Development and Interlaboratory Validation of a Quantitative Antibody Capture ELISA for the Measurement of Specific Guinea-pig IgG1: An Alternative to the Passive Cutaneous Anaphylaxis Assay

1996 ◽  
Vol 24 (1) ◽  
pp. 73-79
Author(s):  
Laura S. Babcock ◽  
Thomas T. Kawabata ◽  
Victor S. Moore ◽  
Catherine M. Condo ◽  
Annette Prentø
Keyword(s):  
Guinea Pig ◽  
Passive Cutaneous Anaphylaxis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Protein Antigens ◽  
Capture Elisa ◽  
Elisa Format ◽  
Interlaboratory Validation ◽  
Antibody Capture ◽  
Development And Validation

Specific guinea-pig IgG1 has traditionally been measured by using an in vivo guinea-pig passive cutaneous anaphylaxis (PCA) assay. This paper describes the development and validation of a quantitative enzyme-linked immunosorbent assay (ELISA) for specific IgG1 against two protein antigens (Alcalase and Enzyme B) as an alternative to the PCA assay. The ELISA format involved a rabbit antibody bound to microtitre plates to capture the antigen. The test sera is added to this, followed sequentially by goat anti-guinea-pig IgG1 and rabbit anti-goat-IgG-alkaline phosphatase conjugate. Aliquots of each serum sample from immunised guinea-pigs were analysed with the ELISA for specific IgG1 titres in three laboratories and were compared to titres determined by using the PCA assay. The findings demonstrate that there is a good correlation between the ELISA and the PCA assay and that the ELISA shows good interlaboratory reproducibility. Thus, the antibody capture ELISA described in this report is a valid and robust replacement for the guinea-pig PCA assay.

scholarly journals Chemical mediators increasing vascular permeability in rat carrageenin pleurisy

1976 ◽  
Vol 26 ◽  
pp. 123
Author(s):  
Kumiko Ikeda ◽  
Yoshiteru Harada ◽  
Kunio Tanaka ◽  
Makoto Katori
Keyword(s):  
Vascular Permeability ◽  
Chemical Mediators

Increase in vascular permeability of rat and guinea pig skin by T-kinin

Inflammation ◽  
1988 ◽  
Vol 12 (5) ◽  
pp. 407-412 ◽  
Author(s):  
K. Sugio ◽  
L. M. Greenbaum
Keyword(s):  
Guinea Pig ◽  
Vascular Permeability ◽  
Pig Skin
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