scholarly journals Determination of the subunit composition of haptoglobin 2-1 polymers using quantitative densitometry of polyacrylamide gels.

1976 ◽  
Vol 251 (19) ◽  
pp. 5845-5851
Author(s):  
D C Hooper ◽  
A C Peacock
Keyword(s):  
Subunit Composition ◽  
Polyacrylamide Gels

A study on the degradation of arachin by proteolytic enzymes

1973 ◽  
Vol 51 (11) ◽  
pp. 2217-2222 ◽  
Author(s):  
R. B. van Huystee
Keyword(s):  
Amino Acids ◽  
Proteolytic Activity ◽  
Free Amino Acids ◽  
Free Amino ◽  
Proteolytic Enzymes ◽  
Disc Electrophoresis ◽  
Polyacrylamide Gels ◽  
Macromolecular Protein

The prime purpose of this proteolysis study was to direct attention to alternate means of measuring proteolytic activity other than the determination of free amino acids. The release of peptides from a macromolecular protein during incubation with either papain, pronase, or trypsin was determined by measuring the presence of 280-nm-absorbing molecules in the fractionation range of Sephadex G 25 eluant after incubation. The formation of larger proteinaceous constituents by proteolysis of arachin was analyzed by disc electrophoresis on polyacrylamide gels. Using these techniques it was noted that papain was the most efficient proteolytic agent for the degradation of arachin.

Determination of rat plasma esterase-1 (ES-1) activity by scanning densitometry of gradient polyacrylamide gels with zymogram detection

1991 ◽  
Vol 12 (12) ◽  
pp. 1045-1050 ◽  
Author(s):  
Hein A. Van Lith ◽  
Manuela Haller ◽  
Bert F. M. Van Zutphen ◽  
Anton C. Beynen
Keyword(s):  
Rat Plasma ◽  
Polyacrylamide Gels ◽  
Scanning Densitometry ◽  
Plasma Esterase

Image Segmentation Applied to Line Separation and Determination of GPN2 Protein Overexpression for Its Detection in Polyacrylamide Gels

2021 ◽  
pp. 303-315
Author(s):  
Jorge Juárez ◽  
María del Rayo Graciela Guevara-Villa ◽  
Anabel Sánchez-Sánchez ◽  
Raquel Díaz-Hernández ◽  
Leopoldo Altamirano-Robles
Keyword(s):  
Image Segmentation ◽  
Polyacrylamide Gels ◽  
Protein Overexpression ◽  
Line Separation

scholarly journals A simple sensitive method, applicable to small polypeptides, for the determination of proteins in polyacrylamide gels after isoelectric focusing

1978 ◽  
Vol 171 (1) ◽  
pp. 79-82 ◽  
Author(s):  
P L Storring ◽  
R J Tiplady
Keyword(s):  
Isoelectric Focusing ◽  
Trichloroacetic Acid ◽  
Polyacrylamide Gels ◽  
Simple Method ◽  
Controlled Conditions ◽  
Sensitive Method

A simple method is described for the determination of polypeptides and proteins in polyacrylamide gels after isoelectric focusing. Precipitates formed in trichloroacetic acid, under controlled conditions, are quantified densitometrically by measuring the proportion of light scattered. The procedure is of particular value in its applicability to smaller polypeptides, with mol.wts. of 3000-6000, which are not fixed adequately in gels by other procedures currently in use. The working range, over which polypeptide concentration is proportional to the effective absorbance, is approx. 1-30 microgram per component.

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