Analysis of human acid beta-glucosidase by site-directed mutagenesis and heterologous expression.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(17)42166-1 ◽

1994 ◽

Vol 269 (3) ◽

pp. 2283-2291

Author(s):

M.E. Grace ◽

K.M. Newman ◽

V. Scheinker ◽

A. Berg-Fussman ◽

G.A. Grabowski

Keyword(s):

Heterologous Expression ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

Beta Glucosidase

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Analyses of catalytic activity and inhibitor binding of human acid beta-glucosidase by site-directed mutagenesis. Identification of residues critical to catalysis and evidence for causality of two Ashkenazi Jewish Gaucher disease type 1 mutations.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(19)39223-3 ◽

1990 ◽

Vol 265 (12) ◽

pp. 6827-6835

Author(s):

M E Grace ◽

P N Graves ◽

F I Smith ◽

G A Grabowski

Keyword(s):

Gaucher Disease ◽

Disease Type ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

Ashkenazi Jewish ◽

Inhibitor Binding ◽

Gaucher Disease Type 1 ◽

Beta Glucosidase

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Heterologous expression of human prostatic acid phosphatase and site-directed mutagenesis of the enzyme active site.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(17)37063-1 ◽

1994 ◽

Vol 269 (12) ◽

pp. 8971-8978

Author(s):

K. Ostanin ◽

A. Saeed ◽

R.L. Van Etten

Keyword(s):

Acid Phosphatase ◽

Heterologous Expression ◽

Active Site ◽

Prostatic Acid Phosphatase ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

Enzyme Active Site

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Heterologous expression and site-directed mutagenesis of endoglucanase CelA from Clostridium thermocellum

Biotechnology Letters ◽

10.1007/s10529-007-9379-5 ◽

2007 ◽

Vol 29 (8) ◽

pp. 1243-1247 ◽

Author(s):

Qiang Yao ◽

Tingting Sun ◽

Guanjun Chen ◽

Weifeng Liu

Keyword(s):

Heterologous Expression ◽

Clostridium Thermocellum ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis

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Heterologous Expression of the Vanadium-containing Chloroperoxidase fromCurvularia inaequalisinSaccharomyces cerevisiaeand Site-directed Mutagenesis of the Active Site Residues His496, Lys353, Arg360, and Arg490

Journal of Biological Chemistry ◽

10.1074/jbc.274.34.23820 ◽

1999 ◽

Vol 274 (34) ◽

pp. 23820-23827 ◽

Author(s):

Wieger Hemrika ◽

Rokus Renirie ◽

Sandra Macedo-Ribeiro ◽

Albrecht Messerschmidt ◽

Ron Wever

Keyword(s):

Heterologous Expression ◽

Active Site ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

Active Site Residues

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A pseudo-β-glucosidase in Arabidopsis thaliana: Correction by site-directed mutagenesis, heterologous expression, purification, and characterization

Biochemistry (Moscow) ◽

10.1134/s0006297908080099 ◽

2008 ◽

Vol 73 (8) ◽

pp. 912-919 ◽

Author(s):

Y. Turan

Keyword(s):

Arabidopsis Thaliana ◽

Heterologous Expression ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

Purification And Characterization

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Functional and structural analyses for MlrC enzyme of Novosphingobium sp. THN1 in microcystin-biodegradation: Involving optimized heterologous expression, bioinformatics and site-directed mutagenesis

Chemosphere ◽

10.1016/j.chemosphere.2020.126906 ◽

2020 ◽

Vol 255 ◽

pp. 126906

Author(s):

Ruiping Wang ◽

Jieming Li ◽

Ji Li

Keyword(s):

Heterologous Expression ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis

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Heterologous expression and site-directed mutagenesis studies of two Trichoderma harzianum chitinases, Chit33 and Chit42, in Escherichia coli

Protein Expression and Purification ◽

10.1016/j.pep.2006.07.020 ◽

2007 ◽

Vol 51 (2) ◽

pp. 216-226 ◽

Author(s):

Harry Boer ◽

Helena Simolin ◽

Sylvain Cottaz ◽

Hans Söderlund ◽

Anu Koivula

Keyword(s):

Escherichia Coli ◽

Heterologous Expression ◽

Trichoderma Harzianum ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis

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Heterologous Expression and Site-Directed Mutagenesis Studies on the Activation Mechanism and the Roles of the Basic Residues in the Prosegment of Aspergillopepsinogen I

European Journal of Biochemistry ◽

10.1111/j.1432-1033.1996.0719p.x ◽

1996 ◽

Vol 237 (3) ◽

pp. 719-725 ◽

Author(s):

Hideshi Inoue ◽

Takaharu Hayashi ◽

Xiang-Ping Huang ◽

Jing-Fang Lu ◽

Senarath B. P. Athauda ◽

...

Keyword(s):

Heterologous Expression ◽

Site Directed Mutagenesis ◽

Activation Mechanism ◽

Directed Mutagenesis

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Valine dehydrogenase fromStreptomyces albus: gene cloning, heterologous expression and identification of active site by site-directed mutagenesis

FEMS Microbiology Letters ◽

10.1111/j.1574-6968.2000.tb08868.x ◽

2000 ◽

Vol 182 (1) ◽

pp. 29-34 ◽

Author(s):

Chang-Gu Hyun ◽

Sang Suk Kim ◽

Kwan-Hyung Park ◽

Joo-Won Suh

Keyword(s):

Heterologous Expression ◽

Gene Cloning ◽

Active Site ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis

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Heterologous expression and site-directed mutagenesis of an ascorbate-reducible cytochrome b561

Archives of Biochemistry and Biophysics ◽

10.1016/j.abb.2005.09.006 ◽

2005 ◽

Vol 443 (1-2) ◽

pp. 82-92 ◽

Author(s):

Alajos Bérczi ◽

Dan Su ◽

Mahadevan Lakshminarasimhan ◽

Amy Vargas ◽

Han Asard

Keyword(s):

Heterologous Expression ◽

Site Directed Mutagenesis ◽

Directed Mutagenesis ◽

Cytochrome B561

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