scholarly journals Bacteriophage P1 site-specific recombination. Purification and properties of the Cre recombinase protein.

1984 ◽  
Vol 259 (3) ◽  
pp. 1509-1514
Author(s):  
K Abremski ◽  
R Hoess
Keyword(s):  
Cre Recombinase ◽  
Site Specific ◽  
Site Specific Recombination ◽  
Bacteriophage P1 ◽  
Purification And Properties

Transgene Coplacement and High Efficiency Site-Specific Recombination With the Cre/loxP System in Drosophila

Genetics ◽  
1996 ◽  
Vol 144 (2) ◽  
pp. 715-726 ◽  
Author(s):  
Mark L Siegal ◽  
Daniel L Hartl
Keyword(s):  
Germ Cells ◽  
Maternal Effect ◽  
High Efficiency ◽  
Loxp Site ◽  
Position Effects ◽  
Site Specific ◽  
Site Specific Recombination ◽  
Bacteriophage P1 ◽  
Eye Color ◽  
F2 Generation

Abstract Studies of gene function and regulation in transgenic Drosophila are often compromised by the possibility of genomic position effects on gene expression. We have developed a method, called transgene coplacement, in which any two sequences can be positioned at exactly the same site and orientation in the genome. Transgene coplacement makes use of the bacteriophage P1 system of Cre/loxP site-specific recombination, which we have introduced into Drosophila. In the presence of a cre transgene driven by a dual hsp70-Mosl promoter, a white reporter gene flanked by loxP sites is excised with virtually 100% efficiency both in somatic cells and in germ cells. A strong maternal effect, resulting from Cre recombinase present in the oocyte, is observed as white or mosaic eye color in F1 progeny. Excision in germ cells of the F1 yields a strong grand-maternal effect, observed as a highly skewed ratio of eye-color phenotypes in the F2 generation. The excision reactions of Cre/loxP and the related FLP/FRT system are used to create Drosophila lines in which transgenes are at exactly allelic sites in homologous chromosomes.

Bacteriophage P1 site-specific recombination

1981 ◽  
Vol 150 (4) ◽  
pp. 487-507 ◽  
Author(s):  
Nat Sternberg ◽  
Daniel Hamilton ◽  
Ronald Hoess
Keyword(s):  
Site Specific ◽  
Site Specific Recombination ◽  
Bacteriophage P1
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