scholarly journals The occurrence of three different proteoglycan species in chick embryo cartilage. Isolation and characterization of a second proteoglycan (PG-Lb) and its precursor form.

1983 ◽  
Vol 258 (15) ◽  
pp. 9314-9322 ◽  
Author(s):  
T Shinomura ◽  
K Kimata ◽  
Y Oike ◽  
A Noro ◽  
N Hirose ◽  
...  
Keyword(s):  
Chick Embryo ◽  
Isolation And Characterization

scholarly journals Studies on sex-organ development. Isolation and characterization of an oestrogen receptor from chick Müllerian duct

1975 ◽  
Vol 150 (2) ◽  
pp. 183-190 ◽  
Author(s):  
C S Teng ◽  
C T Teng
Keyword(s):  
Chick Embryo ◽  
Isoelectric Focusing ◽  
Oestrogen Receptor ◽  
Gradient Analysis ◽  
Hormone Receptors ◽  
Mullerian Duct ◽  
Müllerian Duct ◽  
High Affinity ◽  
Isolation And Characterization

An oestradiol-binding macromolecule was observed in the left Müllerian duct of the 15-day female chick embryo. The embryonic receptor binds oestradiol with a high affinity and low capacity, having a Kd of 3.2 × 10(-9)M and a maximal number of sites of 5.45 fmol/10(6) cells in the left Müllerian duct. The receptor is protein in nature, as suggested by its susceptibility to proteolysis; in addition, it is organ- and steroid-specific. Judging by glycerol-gradient analysis, the hormone receptors in the cytosol are present in 8S and 4.5S forms, and the 8S form could be dissociated into a 4.5S form in the presence of 0.5M-KCl. A 4.5-6S receptor could be extracted from the nuclei. Under physiological salt conditions, the embryonic receptors bind to DNA-cellulose and can be eluted when the salt concentration is increased to 0.5M-KCl. Determination by isoelectric focusing indicates that the isoelectric point is 5.8 for the 8S and 6.9 for the 4.5S receptor.

Isolation and characterization of a vinculin cDNA from chick embryo fibroblasts

1987 ◽  
Vol 15 (5) ◽  
pp. 794-796
Author(s):  
PETER JONES ◽  
GLYN J. PRICE ◽  
MATTHEW D. DAVISON ◽  
DAVID R. CRITCHLEY
Keyword(s):  
Chick Embryo ◽  
Isolation And Characterization ◽  
Embryo Fibroblasts

The isolation and characterization of 18 equine microsatellite loci, TKY272–TKY289

2000 ◽  
Vol 31 (2) ◽  
pp. 149-149 ◽  
Author(s):  
T Tozaki ◽  
H Kakoi ◽  
S Mashima ◽  
K Hirota ◽  
T Hasegawa ◽  
...  
Keyword(s):  
Microsatellite Loci ◽  
Isolation And Characterization
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