scholarly journals KINETIC ANALYSIS OF ENZYME REACTIONS

1953 ◽  
Vol 200 (2) ◽  
pp. 669-682 ◽  
Author(s):  
J.F. Kachmar ◽  
P.D. Boyer
Keyword(s):  
Kinetic Analysis ◽  
Enzyme Reactions

A Kinetic Analysis of Coupled (or Auxiliary) Enzyme Reactions

2018 ◽  
Vol 80 (12) ◽  
pp. 3154-3183 ◽  
Author(s):  
Justin Eilertsen ◽  
Santiago Schnell
Keyword(s):  
Kinetic Analysis ◽  
Enzyme Reactions

scholarly journals Some classical errors in the kinetic analysis of enzyme reactions

1993 ◽  
Vol 295 (3) ◽  
pp. 898-899 ◽  
Author(s):  
K Brocklehurst ◽  
C M Topham
Keyword(s):  
Kinetic Analysis ◽  
Enzyme Reactions

scholarly journals Comments on the kinetic analysis of enzyme reactions involving an unstable irreversible modifier

1992 ◽  
Vol 287 (1) ◽  
pp. 333-334 ◽  
Author(s):  
R Varón ◽  
E Valero ◽  
B Havsteen ◽  
C Garrido ◽  
J N Rodríguez-López ◽  
...  
Keyword(s):  
Kinetic Analysis ◽  
Enzyme Reactions

Kinetic analysis of enzyme reactions with slow-binding inhibition

Biosystems ◽  
1999 ◽  
Vol 51 (3) ◽  
pp. 169-180 ◽  
Author(s):  
Carmelo Garrido-del Solo ◽  
Francisco Garcı́a-Cánovas ◽  
Bent H. Havsteen ◽  
Ramón Varón Castellanos
Keyword(s):  
Kinetic Analysis ◽  
Enzyme Reactions ◽  
Binding Inhibition

Photopatterning Enzymes on Polymer Monoliths in Microfluidic Devices for Steady-State Kinetic Analysis and Spatially Separated Multi-Enzyme Reactions

2007 ◽  
Vol 79 (17) ◽  
pp. 6592-6598 ◽  
Author(s):  
Timothy C. Logan ◽  
Douglas S. Clark ◽  
Timothy B. Stachowiak ◽  
Frantisek Svec ◽  
Jean M. J. Fréchet
Keyword(s):  
Steady State ◽  
Kinetic Analysis ◽  
Microfluidic Devices ◽  
Polymer Monoliths ◽  
Enzyme Reactions ◽  
Steady State Kinetic ◽  
State Kinetic

Kinetic Aspects of the Interaction of Blood Clotting Enzymes

1965 ◽  
Vol 13 (01) ◽  
pp. 155-175 ◽  
Author(s):  
H. C Hemker ◽  
P.W Hemker ◽  
E. A Loeliger
Keyword(s):  
Kinetic Analysis ◽  
Enzyme Kinetics ◽  
Blood Clotting ◽  
Enzyme System ◽  
Enzyme Kinetic ◽  
Clotting Time ◽  
Standard Methods

SummaryApplication of the methods of enzyme-kinetic analysis to the results of clotting tests is feasible and can yield useful results. However, the standard methods of enzyme kinetics are not applicable without modifications imposed by the peculiarities of the blood-clotting enzyme system. The influence of the following complicating circumstances is calculated :1. Substrate is not present in excess.2. Only relative measures exist for concentrations of substrate or enzymes.3. Enzymes and substrates are often added together.4. Reagents are not pure.5. Clotting-time is our only measure for clotting-velocity.Formulas are deduced, which makes it possible to recognize the effect of these complications.

Modeling Trienzyme Biosensor at Internal Diffusion Limitation

2004 ◽  
Vol 9 (2) ◽  
pp. 139-144 ◽  
Author(s):  
J. Kulys
Keyword(s):  
Immobilized Enzymes ◽  
Internal Diffusion ◽  
Significant Response ◽  
Diffusion Limitation ◽  
Enzyme Reactions ◽  
Substrate Conversion ◽  
Apparent Stability ◽  
Biosensor Response

A model of biosensor containing three immobilized enzymes utilizing consecutive substrate conversion in the chain was developed. The modeling was performed at an internal diffusion limitation and a steadystate condition. The calculations showed that significant response of biosensors was produced if diffusion modules were larger than 1 for all enzyme reactions. Due to diffusion limitation the apparent stability of biosensor response increased many times in comparison to stability of the most labile enzyme of the chain.

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