scholarly journals Role of Cytochrome b5 in Hydroxylation by a Reconstituted Cytochrome P-450-containing System

1974 ◽  
Vol 249 (21) ◽  
pp. 6701-6709
Author(s):  
Anthony Y.H. Lu ◽  
Susan B. West ◽  
Mary Vore ◽  
Dene Ryan ◽  
Wayne Levin
Keyword(s):  
Cytochrome B5 ◽  
Cytochrome P 450

The Role of Cytochrome b5 in Cytochrome P-450 Enzymes

1975 ◽  
pp. 435-445 ◽  
Author(s):  
H. A. Sasame ◽  
S. S. Thorgeirsson ◽  
J. R. Mitchell ◽  
J. R. Gillette
Keyword(s):  
Cytochrome B5 ◽  
Cytochrome P 450

Role of cytochrome b5 in the cytochrome P-450-mediated C21-steroid 17,20-lyase reaction

1982 ◽  
Vol 108 (1) ◽  
pp. 379-384 ◽  
Author(s):  
Masayuki Katagiri ◽  
Katsuko Suhara ◽  
Masahiro Shiroo ◽  
Yoshiyuki Fujimura
Keyword(s):  
Cytochrome B5 ◽  
Cytochrome P 450

scholarly journals Decrease in hepatic cytochrome P-450 by cobalt. Evidence for a role of cobalt protoporphyrin

1982 ◽  
Vol 204 (1) ◽  
pp. 103-109 ◽  
Author(s):  
J F Sinclair ◽  
P R Sinclair ◽  
J F Healey ◽  
E L Smith ◽  
H L Bonkowsky
Keyword(s):  
Chick Embryo ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Polyacrylamide Gel ◽  
Cobalt Protoporphyrin ◽  
Cytochrome P 450 ◽  
Hepatic Cytochrome

Exposure of cultured chick-embryo hepatocytes to increasing concentrations of CoCl2 in the presence of allylisopropylacetamide results in formation of cobalt protoporphyrin, with a reciprocal decrease in haem and cytochrome P-450. Treatment of rats with CoCl2 (84 mumol/kg) and 5-aminolaevulinate (0.2 mmol/kg) also results in formation of cobalt protoporphyrin and a decrease in cytochrome P-450 in the liver. Hepatic microsomal fractions from rats treated with phenobarbital, CoCl2 and 5-aminolaevulinate were analysed by polyacrylamide gel electrophoresis. Cobalt protoporphyrin was associated mainly with proteins of 50000-53000 mol.wt. The results suggest that the formation of cobalt protoporphyrin occurred at the expense of the synthesis of haem, leading to a decrease in cytochrome P-450. Furthermore, the cobalt protoporphyrin that was formed may itself have been incorporated into apocytochrome P-450.

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