Impact of triethylamine as a mobile phase additive on the resolution of racemic amino acids on an (+)-18-crown-6-tetracarboxylic acid-derived chiral stationary phase

2001 ◽  
Vol 933 (1-2) ◽  
pp. 83-90 ◽  
Author(s):  
Jong Sung Jin ◽  
Apryll M. Stalcup ◽  
Myung Ho Hyun
Keyword(s):  
Amino Acids ◽  
Stationary Phase ◽  
Mobile Phase ◽  
Chiral Stationary Phase ◽  
Tetracarboxylic Acid ◽  
Mobile Phase Additive

scholarly journals HIGH – PERFORMANCE LIQUID CHROMATOGRAPHIC METHOD FOR ENANTIOSEPARATION OF UNDERIVATIZED α-AMINO ACIDS USING CYCLOFRUCTAN – BASED CHIRAL STATIONARY PHASES

2013 ◽  
Vol 12 (2) ◽  
pp. 108-119 ◽  
Author(s):  
Jakub Moravčík ◽  
Katarína Hroboňová
Keyword(s):  
Amino Acids ◽  
Stationary Phase ◽  
Mobile Phase ◽  
High Performance ◽  
Chiral Stationary Phase ◽  
Stationary Phases ◽  
Chiral Stationary Phases ◽  
High Performance Liquid Chromatographic ◽  
Optical Isomers ◽  
Chiral Amino Acids

Abstract Chromatographic columns with chiral stationary phases based on chemically - bonded derivatized cyclofructans were evaluated for HPLC enantioresolution of underivatized α-amino acids. The analytical study of chiral separation of these analytes was focused on the selection of suitable chiral stationary phase and mobile phase. Using isopropyl carbamate cyclofructan 6 as a chiral stationary phase, α- amino acid optical isomers were separated. The retention and enantioseparation of chiral amino acids were also influenced by a mobile phase composition. The mixture methanol/acetonitrile/acetic acid/triethylamine (75/25/0.3/0.2 v/v/v/v) was found to be the most effective mobile phase for HPLC separation of studied compounds. HPLC enantioresolution of chiral amino acids was thermodynamically studied. Based on the enthalpy and entropy contribution values calculated from the van´t Hoff equation, HPLC enantioseparation under chosen chromatographic conditions was found to be an enthalpically driven.

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