Chiral discrimination ofN-(dansyl)-dl-amino acids on human serum albumin stationary phase: Effect of a mobile phase modifier

1998 ◽  
Vol 48 (5-6) ◽  
pp. 431-435 ◽  
Author(s):  
E. Peyrin ◽  
Y. C. Guillaume
Keyword(s):  
Amino Acids ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Stationary Phase ◽  
Human Serum ◽  
Mobile Phase ◽  
Chiral Discrimination ◽  
Phase Effect ◽  
Mobile Phase Modifier

Chiral discrimination of phenoxypropionic acid herbicides on teicoplanin phase: Effect of mobile phase modifier

2002 ◽  
Vol 55 (3-4) ◽  
pp. 143-148 ◽  
Author(s):  
Y. C. Guillaume ◽  
T. T. Truong ◽  
J. Millet ◽  
L. Nicod ◽  
C. Guinchard ◽  
...  
Keyword(s):  
Mobile Phase ◽  
Chiral Discrimination ◽  
Phase Effect ◽  
Mobile Phase Modifier ◽  
Acid Herbicides

In vitro stereoselective degradation of carprofen glucuronide by human serum albumin. Characterization of sites and reactive amino acids

Chirality ◽  
2000 ◽  
Vol 12 (2) ◽  
pp. 53-62 ◽  
Author(s):  
H�l�ne Georges ◽  
Nathalie Presle ◽  
Thierry Buronfosse ◽  
Sylvie Fournel-Gigleux ◽  
Patrick Netter ◽  
...  
Keyword(s):  
Amino Acids ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Stereoselective Degradation

Exploring the Molecular Level Interaction of Human Serum Albumin with Calcium Oxalate Monohydrate Crystals

2021 ◽  
Vol 28 ◽  
Author(s):  
Priyadarshini ◽  
Abhishek Negi ◽  
Chetna Faujdar ◽  
Lokesh Nigam ◽  
Naidu Subbarao
Keyword(s):  
Amino Acids ◽  
Crystal Growth ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Calcium Oxalate ◽  
Human Serum ◽  
Stone Formation ◽  
Calcium Oxalate Monohydrate ◽  
Calcium Oxalate Crystallization ◽  
In Silico Docking

Background: Human serum albumin (HSA) is one of the most abundant proteins in the blood plasma, urine as well as in the organic matrix of renal calculi. Macromolecules present in the urine modulate kidney stone formation either by stimulating or inhibiting crystallization process. Objective: In the present study, effect of HSA protein on the growth of calcium oxalate monohydrate crystal (COM) was investigated. Methods: Crystal growth assay was used to measure oxalate depletion in the crystal seeded solution in the presence of HSA. HSA concentrations exhibiting effect on crystal growth were selected for FTIR and XRD analysis. In silico docking was performed on seven different binding sites of HSA. Results: Albumin is playing dual role in growth of calcium oxalate crystallization. FTIR and XRD studies further revealed HSA exerted strain over crystal thus affecting its structure by interacting with amino acids of its pocket 1. Docking results indicate that out of 7 binding pocket in protein, calcium oxalate interacts with Arg-186 and Lys-190 amino acids of pocket 1. Conclusion: Our study confirms the role of HSA in calcium oxalate crystallization where acidic amino acids arginine and lysine are binding with COM crystals, revealing molecular interaction of macromolecule and crystal in urolithiasis.

Sucrose Dependence of Solute Retention on Human Serum Albumin Stationary Phase:  Hydrophobic Effect and Surface Tension Considerations

1998 ◽  
Vol 70 (14) ◽  
pp. 2812-2818 ◽  
Author(s):  
Eric Peyrin ◽  
Yves Claude Guillaume ◽  
Nadia Morin ◽  
Christiane Guinchard
Keyword(s):  
Surface Tension ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Stationary Phase ◽  
Human Serum ◽  
Hydrophobic Effect ◽  
Solute Retention
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