Effects of ammonioalkyl sulfonate internal salts on electrokinetic micropump performance and reversed-phase high-performance liquid chromatographic separations

2003 ◽  
Vol 1013 (1-2) ◽  
pp. 93-101 ◽  
Author(s):  
David S. Reichmuth ◽  
Brian J. Kirby
Keyword(s):  
High Performance ◽  
High Performance Liquid Chromatographic ◽  
Reversed Phase ◽  
Chromatographic Separations ◽  
Liquid Chromatographic

Temperature as a variable in reversed-phase high-performance liquid chromatographic separations of peptide and protein samples

1994 ◽  
Vol 686 (1) ◽  
pp. 45-59 ◽  
Author(s):  
Rosanne C. Chloupek ◽  
William S. Hancock ◽  
Brian A. Marchylo ◽  
Joseph J. Kirkland ◽  
Barry E. Boyes ◽  
...  
Keyword(s):  
High Performance ◽  
High Performance Liquid Chromatographic ◽  
Reversed Phase ◽  
Chromatographic Separations ◽  
Liquid Chromatographic

scholarly journals Column High-Performance Liquid Chromatographic Determination of Norfloxacin and Its Main Impurities in Pharmaceuticals

2008 ◽  
Vol 91 (2) ◽  
pp. 332-338 ◽  
Author(s):  
Branislava Mielji ◽  
Gordana Popovi ◽  
Danica Agbaba ◽  
Slavko Markovi ◽  
Breda Simonovska ◽  
...  
Keyword(s):  
High Performance ◽  
Raw Materials ◽  
Chromatographic Determination ◽  
High Performance Liquid Chromatographic ◽  
Reversed Phase ◽  
Array Detector ◽  
Chromatographic Separations ◽  
Experimental Conditions ◽  
Relative Standard ◽  
Liquid Chromatographic

Abstract A gradient reversed-phase column high-performance liquid chromatographic method was developed for the detection and quantification of norfloxacin and its major impurities in norfloxacin-containing pharmaceuticals. Chromatographic separations were performed under the following experimental conditions: column, Zorbax SB RP-18 (5 m, 250 4.6 mm); injection volume, 20 L; mobile phase, 0.05 M NaH2PO4 (pH 2.5)acetonitrile (87 + 13) for 16 min and (58 + 42) for 9 min (stepwise gradient); and flow rate, 1.3 mL/min. All analyses were performed at 25C, and the eluate was monitored at 275 nm using a diode array detector. Linearity (correlation coefficient = 0.999), recovery (99.3101.8), relative standard deviation (0.20.7), and quantitation limit (0.120.47 g/mL) were evaluated and found to be satisfactory. The method is simple, rapid, and convenient for purity control of norfloxacin in both raw materials and dosage forms.

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