16S rRNA Restriction Fragment Length Polymorphism Analysis of Bacterial Diversity as a Biomarker of Ecological Health in Polluted Sediments from New Bedford Harbor, Massachusetts, USA

1999 ◽  
Vol 38 (8) ◽  
pp. 663-675 ◽  
Author(s):  
Jonathan J. Sorci ◽  
Joseph D. Paulauskis ◽  
Timothy E. Ford
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
16S Rrna ◽  
Bacterial Diversity ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Polymorphism Analysis ◽  
Ecological Health ◽  
New Bedford Harbor ◽  
Restriction Fragment Length

scholarly journals Detection and Characterization of a Dehalogenating Microorganism by Terminal Restriction Fragment Length Polymorphism Fingerprinting of 16S rRNA in a Sulfidogenic, 2-Bromophenol-Utilizing Enrichment

2004 ◽  
Vol 70 (2) ◽  
pp. 1169-1175 ◽  
Author(s):  
Donna E. Fennell ◽  
Sung-Keun Rhee ◽  
Young-Beom Ahn ◽  
Max M. Häggblom ◽  
Lee J. Kerkhof
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
16S Rrna ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Reductive Dehalogenation ◽  
Polymorphism Analysis ◽  
Sulfate Reducing ◽  
Restriction Fragment Length

ABSTRACT Terminal restriction fragment length polymorphism analysis of reverse-transcribed 16S rRNA during periods of community flux was used as a tool to delineate the roles of the members of a 2-bromophenol-degrading, sulfate-reducing consortium. Starved, washed cultures were amended with 2-bromophenol plus sulfate, 2-bromophenol plus hydrogen, phenol plus sulfate, or phenol with no electron acceptor and were monitored for substrate use. In the presence of sulfate, 2-bromophenol and phenol were completely degraded. In the absence of sulfate, 2-bromophenol was dehalogenated and phenol accumulated. Direct terminal restriction fragment length polymorphism fingerprinting of the 16S rRNA in the various subcultures indicated that phylotype 2BP-48 (a Desulfovibrio-like sequence) was responsible for the dehalogenation of 2-bromophenol. A stable coculture was established which contained predominantly 2BP-48 and a second Desulfovibrio-like bacterium (designated BP212 based on terminal restriction fragment length polymorphism fingerprinting) that was capable of dehalogenating 2-bromophenol to phenol. Strain 2BP-48 in the coculture could couple reductive dehalogenation to growth with 2-bromophenol, 2,6-dibromophenol, or 2-iodophenol and lactate or formate as the electron donor. In addition to halophenols, strain 2BP-48 appears to use sulfate, sulfite, and thiosulfate as electron acceptors and is capable of simultaneous sulfidogenesis and reductive dehalogenation in the presence of sulfate.

scholarly journals Fecal Bacterial Diversity in a Wild Gorilla

2006 ◽  
Vol 72 (5) ◽  
pp. 3788-3792 ◽  
Author(s):  
Julie C. Frey ◽  
Jessica M. Rothman ◽  
Alice N. Pell ◽  
John Bosco Nizeyi ◽  
Michael R. Cranfield ◽  
...  
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Bacterial Diversity ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Gene Clone ◽  
Rrna Gene ◽  
Restriction Fragment Length

ABSTRACT We describe the bacterial diversity in fecal samples of a wild gorilla by use of a 16S rRNA gene clone library and terminal-restriction fragment length polymorphism (T-RFLP). Clones were classified as Firmicutes, Verrucomicrobia, Actinobacteria, Lentisphaerae, Bacteroidetes, Spirochetes, and Planctomycetes. Our data suggest that fecal populations did not change temporally, as determined by T-RFLP.

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