Evaluation of dipsticks in Schistosoma haematobium infections in four villages in the middle valley of the Senegal River Basin, Senegal

ABSTRACTHybridization events between Schistosoma species (Digenea, Platyhelminthes) are reported with increasing frequency, largely due to improved access to molecular tools. Nevertheless, little is known about the distribution and frequency of hybrid schistosomes in nature. Screening for hybrids on a large scale is complicated by the need for nuclear and mitochondrial sequence information, precluding a ‘simple’ barcoding approach. Here we aimed to determine and understand the spatiotemporal distribution of Schistosoma haematobium × Schistosoma bovis hybrids in the Senegal River Basin. From ten villages, distributed over the four main water basins, we genotyped a total of 1236 schistosome larvae collected from human urine samples using a partial mitochondrial cox1 fragment; a subset of 268 parasites was also genotyped using ITS rDNA. Hybrid schistosomes were unevenly distributed, with substantially higher numbers in villages bordering Lac de Guiers than in villages from the Lampsar River and the Middle Valley of the Senegal River. The frequency of hybrids per village was not linked with the prevalence of urinary schistosomiasis in that village. However, we did find a significant positive association between the frequency of hybrids per village and the prevalence of Schistosoma mansoni. We discuss the potential consequences of adopting a barcoding approach when studying hybrids in nature.

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The epidemiology of a recent focus of mixed Schistosoma haematobium and Schistosoma mansoni infections around the 'Lac de Guiers' in the Senegal River Basin, Senegal

Tropical Medicine & International Health ◽

10.1046/j.1365-3156.1999.00444.x ◽

1999 ◽

Vol 4 (8) ◽

pp. 544-550 ◽

Cited By ~ 22

Author(s):

D. Clercq ◽

J. Vercruysse ◽

M. Picquet ◽

D. J. Shaw ◽

M. Diop ◽

...

Keyword(s):

Schistosoma Mansoni ◽

River Basin ◽

Schistosoma Haematobium ◽

Senegal River ◽

Senegal River Basin

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Urogenital schistosomiasis and hybridization between Schistosoma haematobium and Schistosoma bovis in adults living in Richard-Toll, Senegal

Parasitology ◽

10.1017/s0031182018001415 ◽

2018 ◽

Vol 145 (13) ◽

pp. 1723-1726 ◽

Cited By ~ 5

Author(s):

Mariama Sene-Wade ◽

Bernard Marchand ◽

David Rollinson ◽

Bonnie L. Webster

Keyword(s):

Genetic Analysis ◽

River Basin ◽

Schistosoma Haematobium ◽

Local Hospital ◽

Urogenital Schistosomiasis ◽

Senegal River ◽

Schistosoma Bovis ◽

Senegal River Basin

AbstractSince the construction of the Diama Dam (1985), the epidemiology of schistosomiasis along the Senegal River Basin (SRB) has been extremely dynamic with outbreaks of both intestinal and urogenital schistosomiasis. In the early 2000s, technicians reported cases of suspected urogenital schistosomiasis in adults from the local hospital in Richard-Toll, Lower SRB. The genetic analysis of schistosome miracidia isolated from 11 patients in 2012 from two neighbourhoods (Campement and Gaya) of Richard-Toll confirmed infection with Schistosoma haematobium but also S. haematobium/S. bovis hybrids. Thirty-seven per cent of the miracidia were S. bovis/S. haematobium hybrids and 63% were pure S. haematobium. The data are discussed in relation to the ongoing dynamic epidemiology of the schistosomes in Senegal and the need to treat non-target individuals.

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