Large-scale purification of rat brain nitric oxide synthase from baculovirus overexpression system

1996 ◽  
pp. 420-427 ◽  
Author(s):  
Bernd Mayer ◽  
Peter Klatt ◽  
Barbara M. List ◽  
Christian Harteneck ◽  
Kurt Schmidt
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Rat Brain ◽  
Large Scale ◽  
Overexpression System ◽  
Oxide Synthase ◽  
Brain Nitric Oxide

scholarly journals Expression of rat brain nitric oxide synthase in baculovirus-infected insect cells and characterization of the purified enzyme

1994 ◽  
Vol 304 (3) ◽  
pp. 683-686 ◽  
Author(s):  
C Harteneck ◽  
P Klatt ◽  
K Schmidt ◽  
B Mayer
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Rat Brain ◽  
Insect Cells ◽  
Specific Activity ◽  
Apparent Homogeneity ◽  
Infected Insect ◽  
High Level ◽  
Oxide Synthase ◽  
Brain Nitric Oxide

Rat brain nitric oxide synthase was expressed to a high level in baculovirus-infected insect cells and purified to apparent homogeneity by affinity chromatography. The enzyme had a specific activity of approximately 1 mumol of citrulline.min-1.mg of protein-1 and contained 0.93, 0.45, 0.18 and 0.23 mol of haem, (6R)-5,6,7,8-tetrahydro-L-biopterin (H4biopterin), FAD and FMN per mol of subunit respectively.

Marine biomolecules inhibit rat brain nitric oxide synthase activity

Toxicology ◽  
1998 ◽  
Vol 129 (2-3) ◽  
pp. 103-112 ◽  
Author(s):  
J. Venkateswara Rao ◽  
D. Desaiah ◽  
P.J.S. Vig ◽  
Y. Venkateswarlu
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Rat Brain ◽  
Oxide Synthase ◽  
Brain Nitric Oxide ◽  
Nitric Oxide Synthase Activity

The Role of Thiols in the Apparent Activation of Rat Brain Nitric Oxide Synthase (NOS)1

1995 ◽  
Vol 117 (4) ◽  
pp. 923-927 ◽  
Author(s):  
Yumiko Komori ◽  
Jinjoo Hyun ◽  
Ken Chiang ◽  
Jon M. Fukuto
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Rat Brain ◽  
Oxide Synthase ◽  
Brain Nitric Oxide

Differential action of 7-nitro indazole on rat brain nitric oxide synthase

1996 ◽  
Vol 219 (2) ◽  
pp. 75-78 ◽  
Author(s):  
Bettina E. Kalisch ◽  
Bruce P. Connop ◽  
Khem Jhamandas ◽  
Richard J. Beninger ◽  
Roland J. Boegman
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Rat Brain ◽  
Oxide Synthase ◽  
Brain Nitric Oxide ◽  
Differential Action

Differential changes in rat brain nitric oxide synthase in vivo and in vitro by methylmercury

1998 ◽  
Vol 798 (1-2) ◽  
pp. 147-155 ◽  
Author(s):  
Masaru Shinyashiki ◽  
Yoshito Kumagai ◽  
Hiromi Nakajima ◽  
Jun Nagafune ◽  
Shino Homma-Takeda ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Rat Brain ◽  
Oxide Synthase ◽  
Brain Nitric Oxide
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