Expression of rat brain nitric oxide synthase in baculovirus-infected insect cells and characterization of the purified enzyme
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Rat brain nitric oxide synthase was expressed to a high level in baculovirus-infected insect cells and purified to apparent homogeneity by affinity chromatography. The enzyme had a specific activity of approximately 1 mumol of citrulline.min-1.mg of protein-1 and contained 0.93, 0.45, 0.18 and 0.23 mol of haem, (6R)-5,6,7,8-tetrahydro-L-biopterin (H4biopterin), FAD and FMN per mol of subunit respectively.
1994 ◽
Vol 81
(SUPPLEMENT)
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pp. A807
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1993 ◽
Vol 307
(2)
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pp. 311-315
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1995 ◽
Vol 117
(4)
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pp. 923-927
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1996 ◽
Vol 219
(2)
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pp. 75-78
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1995 ◽
Vol 206
(2)
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pp. 511-517
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1996 ◽
Vol 209
(3)
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pp. 169-172
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