Minute Virus of Mice cis-Acting Sequences Required for Genome Replication and the Role of the trans-Acting Viral Protein, NS-1

1996 ◽  
pp. 245-285 ◽  
Author(s):  
Caroline R. Astell ◽  
Qingquan Liu ◽  
Colin E. Harris ◽  
John Brunstein ◽  
Hitesh K. Jindal ◽  
...  
Keyword(s):  
Viral Protein ◽  
Genome Replication ◽  
Minute Virus Of Mice ◽  
Cis Acting ◽  
Minute Virus

scholarly journals Cloning and Sequencing of Defective Particles Derived from the Autonomous Parvovirus Minute Virus of Mice for the Construction of Vectors with Minimal cis-Acting Sequences

2001 ◽  
Vol 75 (3) ◽  
pp. 1284-1293 ◽  
Author(s):  
Nathalie Clément ◽  
Bernard Avalosse ◽  
Karim El Bakkouri ◽  
Thierry Velu ◽  
Annick Brandenburger
Keyword(s):  
Virus Production ◽  
Dna Amplification ◽  
Wild Type ◽  
Nonstructural Proteins ◽  
Helper Plasmid ◽  
Minute Virus Of Mice ◽  
Coding Sequences ◽  
Cis Acting ◽  
Minute Virus ◽  
Helper Plasmids

ABSTRACT The production of wild-type-free stocks of recombinant parvovirus minute virus of mice [MVM(p)] is difficult due to the presence of homologous sequences in vector and helper genomes that cannot easily be eliminated from the overlapping coding sequences. We have therefore cloned and sequenced spontaneously occurring defective particles of MVM(p) with very small genomes to identify the minimalcis-acting sequences required for DNA amplification and virus production. One of them has lost all capsid-coding sequences but is still able to replicate in permissive cells when nonstructural proteins are provided in trans by a helper plasmid. Vectors derived from this particle produce stocks with no detectable wild-type MVM after cotransfection with new, matched, helper plasmids that present no homology downstream from the transgene.

scholarly journals Minute virus of mice small non-structural protein NS2 localizes within, but is not required for the formation of, Smn-associated autonomous parvovirus-associated replication bodies

2005 ◽  
Vol 86 (4) ◽  
pp. 1009-1014 ◽  
Author(s):  
Philip J. Young ◽  
Ann Newman ◽  
Klaus T. Jensen ◽  
Lisa R. Burger ◽  
David J. Pintel ◽  
...  
Keyword(s):  
Virus Replication ◽  
Survival Motor Neuron ◽  
Structural Proteins ◽  
Temporal Delay ◽  
Structural Protein ◽  
Wild Type ◽  
Minute Virus Of Mice ◽  
Minute Virus ◽  
Nuclear Structures

The non-structural proteins NS1 and NS2 of the parvovirus minute virus of mice (MVM) are required for efficient virus replication. It has previously been shown that NS1 and NS2 interact and colocalize with the survival motor neuron (Smn) gene product in novel nuclear structures that are formed late in infection, termed Smn-associated APAR (autonomous parvovirus-associated replication) bodies (SAABs). It is not clear what molecular viral intermediate(s) contribute to SAAB formation. The current results address the role of NS2 in SAAB formation. In highly synchronized wild-type MVM infection of murine A92L cells, NS2 colocalizes with Smn and other SAAB constituents. An MVM mutant that does not produce NS2 still generates SAABS, albeit with a temporal delay. The lag in SAAB formation seen in the absence of NS2 is probably related to the temporal delay in virus replication, suggesting that, whilst NS2 is required for efficient viral infection, it is dispensable for SAAB formation.

scholarly journals Identification of the Sialic Acid Structures Recognized by Minute Virus of Mice and the Role of Binding Affinity in Virulence Adaptation

2006 ◽  
Vol 281 (35) ◽  
pp. 25670-25677 ◽  
Author(s):  
Hyun-Joo Nam ◽  
Brittney Gurda-Whitaker ◽  
Wand Yee Gan ◽  
Shawen Ilaria ◽  
Robert McKenna ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Binding Affinity ◽  
Minute Virus Of Mice ◽  
Minute Virus

A precise map of splice junctions in the mRNAs of minute virus of mice, an autonomous parvovirus.

1986 ◽  
Vol 59 (3) ◽  
pp. 564-573 ◽  
Author(s):  
C V Jongeneel ◽  
R Sahli ◽  
G K McMaster ◽  
B Hirt
Keyword(s):  
Minute Virus Of Mice ◽  
Minute Virus ◽  
Splice Junctions
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