Chapter 11 Interpretation of Fluorescence Correlation Spectroscopy and Photobleaching Recovery in Terms of Molecular Interactions

1989 ◽  
pp. 307-332 ◽  
Author(s):  
Elliot. L. Elson ◽  
Hong Qian
Keyword(s):  
Molecular Interactions ◽  
Fluorescence Correlation Spectroscopy ◽  
Correlation Spectroscopy ◽  
Fluorescence Correlation ◽  
Photobleaching Recovery

Fluorescence microscopic analysis of molecular interactions in 3-dimensional samples by means of fluorescence correlation spectroscopy and photobleaching recovery

1988 ◽  
Vol 46 ◽  
pp. 38-39
Author(s):  
Hong Qian ◽  
Elliot L. Elson
Keyword(s):  
Fluorescence Correlation Spectroscopy ◽  
Chemical Interaction ◽  
Microscopic Analysis ◽  
Correlation Spectroscopy ◽  
3 Dimensional ◽  
Fluorescence Correlation ◽  
Fluorescent Materials ◽  
Photobleaching Recovery ◽  
Open Region ◽  
Fluorescence Microscopic Analysis

Fluorescence correlation spectroscopy (FCS) and fluoresence photobleaching recovery (FPR) are closely related methods which can be used to measure rates of transport (e.g. diffusion) and of chemical interaction of fluorescent materials. Both methods rely on measurements of fluorescence excited in an open region of the sample. As molecules are transported into or out of the region or their optical properties are changed by chemical interactions, the measured fluorescence changes correspondingly. Once the size of the illuminated region is known, the rates of transport (diffusion, drift, or flow) may be determined from the measured rates of change of fluorescence. There is, however, and important difference in principle between FCS and FPR. The former extracts rate information from measurements of spontaneous concentration fluctuations. The latter measures the relaxation of a macroscopic concentration gradient produced by photobleaching a portion of the fluorophores in the observation region with a brief high intensity light pulse.

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