scholarly journals Diagnosis of malaria by detection of Plasmodium falciparum HRP-2 antigen with a rapid dipstick antigen-capture assay

The Lancet ◽  
1994 ◽  
Vol 343 (8897) ◽  
pp. 564-568 ◽  
Author(s):  
C Beadle ◽  
G.W Long ◽  
P.D McElroy ◽  
S.L Hoffman ◽  
G.W Long ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Capture Assay ◽  
Antigen Capture

ACIDIFICATION MODIFIED P24 ANTIGEN CAPTURE ASSAY IN HIV-SEROPOSITIVE PATIENTS

1992 ◽  
Vol 85 (Supplement) ◽  
pp. 3S-42
Author(s):  
David Ascher ◽  
Chet Roberts ◽  
Arnold Fowler
Keyword(s):  
Capture Assay ◽  
Antigen Capture ◽  
P24 Antigen ◽  
Hiv Seropositive

Hybridoma Screening by Antigen Capture: Capture or Sandwich ELISA in 96-Well Plates

2022 ◽  
Vol 2022 (1) ◽  
pp. pdb.prot103127
Author(s):  
Edward A. Greenfield
Keyword(s):  
Culture Supernatant ◽  
Detection Method ◽  
Specific Activity ◽  
Binding Capacity ◽  
Sandwich Elisa ◽  
High Specific Activity ◽  
Tissue Culture Supernatant ◽  
Capture Assay ◽  
Antigen Capture ◽  
Elisa Plate

In an antigen capture assay for hybridoma screening, the detection method identifies the presence of the antigen. Often this is achieved by labeling the antigen directly. In this assay, the polyvinyl chloride (PVC) wells of a high-binding-capacity ELISA plate are first coated with an affinity-purified rabbit anti-mouse immunoglobulin and then incubated with hybridoma tissue culture supernatant. Monoclonal antibodies in the supernatant are “captured” on the coated PVC surface and detected by screening with biotin- or histidine (His)–tagged antigen. The antigen can be labeled to a high specific activity and thus very little antigen is required for this procedure.

A Plasmodium falciparum-specific reverse target capture assay

1991 ◽  
Vol 44 (2) ◽  
pp. 165-173 ◽  
Author(s):  
Guo-Xian Chen ◽  
Jingdon Zhu ◽  
James R. Plitt ◽  
Anita K. Weiler ◽  
J. Werner Zolg
Keyword(s):  
Plasmodium Falciparum ◽  
Capture Assay ◽  
Target Capture
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