Clinical usefulness of PIVKA-II measured by sensitive enzyme immunoassay and AFP-L3 measured by automated analyzer in the diagnosis of hepatocellular carcinoma (HCC)

2001 ◽  
Vol 34 (0) ◽  
pp. 98-99
Author(s):  
Y Paik
Keyword(s):  
Hepatocellular Carcinoma ◽  
Enzyme Immunoassay ◽  
Clinical Usefulness

scholarly journals Clinical Usefulness of Components of the Triage Immunoassay, Enzyme Immunoassay for Toxins A and B, and Cytotoxin B Tissue Culture Assay for the Diagnosis of Clostridium difficile Diarrhea

2003 ◽  
Vol 119 (1) ◽  
pp. 45-49 ◽  
Author(s):  
Viki Massey, ART ◽  
Zafar Hussain, MD, FRCP(C) ◽  
Daniel B. Gregson, MD, FRCP(C) ◽  
Michael A. John, MBChB, FRCP(C) ◽  
Abdul H. Chagla, PhD ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Clostridium Difficile ◽  
Enzyme Immunoassay ◽  
Clinical Usefulness

scholarly journals Establishment of an Enzyme Immunoassay Using Monoclonal Antibody (HG1-219) and Its Application for the Diagnosis of Hepatocellular Carcinoma

1992 ◽  
Vol 83 (12) ◽  
pp. 1366-1372 ◽  
Author(s):  
Toshikazu Hada ◽  
Yumiko Kinoshita ◽  
Yuzo Ichimori ◽  
Hiroyasu Imanishi ◽  
Keishi Umeyama ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Monoclonal Antibody ◽  
Enzyme Immunoassay

Tumor-detecting capacity and clinical usefulness of SPIO-MRI in patients with hepatocellular carcinoma

2000 ◽  
Vol 35 (11) ◽  
pp. 849-855 ◽  
Author(s):  
Hiroshi Nakamura ◽  
Naoki Ito ◽  
Fumio Kotake ◽  
Yuji Mizokami ◽  
Takeshi Matsuoka
Keyword(s):  
Hepatocellular Carcinoma ◽  
Clinical Usefulness

Clinical usefulness of des-?-carboxy prothrombin assay in early diagnosis of hepatocellular carcinoma

1991 ◽  
Vol 36 (12) ◽  
pp. 1787-1792 ◽  
Author(s):  
Shigetoshi Fujiyama ◽  
Kiyonori Izuno ◽  
Kazuto Gohshi ◽  
Junji Shibata ◽  
Tatsuo Sato
Keyword(s):  
Hepatocellular Carcinoma ◽  
Early Diagnosis ◽  
Clinical Usefulness

scholarly journals Abundance of Immunologically Active Alanine Aminotransferase in Sera of Liver Cirrhosis and Hepatocellular Carcinoma Patients

2009 ◽  
Vol 55 (5) ◽  
pp. 1022-1025 ◽  
Author(s):  
Hyun Jeong Kim ◽  
Sang Wook Oh ◽  
Dong Joon Kim ◽  
Eui Yul Choi
Keyword(s):  
Hepatocellular Carcinoma ◽  
Liver Cirrhosis ◽  
Liver Disease ◽  
Enzymatic Activity ◽  
Enzyme Immunoassay ◽  
Roc Curve ◽  
Alanine Aminotransferase ◽  
Liver Diseases ◽  
Serum Samples ◽  
Sandwich Enzyme Immunoassay

Abstract Background: Although alanine aminotransferase (ALT) is a widely used indicator of liver function, ALT enzymatic activity may not always reflect the degree of liver damage. Improved methods or approaches would be useful. Methods: Monoclonal antibodies (mAbs) to ALT were generated to develop a sandwich enzyme immunoassay system. We used an immunoassay to measure ALT mass concentration and a common biochemical analyzer to assay ALT enzymatic activity in serum samples from patients with liver diseases and healthy individuals. The results from the 2 methods were compared and analyzed by ROC curve analysis. Results: The ALT sandwich enzyme immunoassay system demonstrated reliable performance in linearity, recovery, and imprecision studies. The ALT activity assay exhibited a higher diagnostic accuracy in acute hepatitis (AH) patients, but the ALT immunoassay exhibited higher sensitivity and specificity in patients with chronic liver diseases. The areas under the ROC curve for ALT mass and enzymatic activity were 0.82 and 0.98, respectively, in AH, 0.99 and 0.52 in hepatocellular carcinoma (HCC), and 0.94 and 0.45 in liver cirrhosis (LC). Serum samples from HCC and LC patients had higher amounts of ALT–immunoglobulin complexes [median A450, 1.7 (interquartile range, 1.4–1.9)] than the other groups [1.3 (interquartile range, 0.9–1.6)]. Conclusions: Our analysis of sera from the HCC and LC patient groups revealed considerable amounts of immunologically active but catalytically inactive ALT. The amount of the ALT–immunoglobulin complex increased with the severity of the liver disease. The 2-site immunoassay method may be useful in the differential diagnosis of some causes of liver disease.

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