Isolation and characterization of rice lesion-mimic mutants with enhanced resistance to rice blast and bacterial blight

Plant Science ◽  
2002 ◽  
Vol 163 (2) ◽  
pp. 345-353 ◽  
Author(s):  
Ritsuko Mizobuchi ◽  
Hideyuki Hirabayashi ◽  
Ryota Kaji ◽  
Yoko Nishizawa ◽  
Atsushi Yoshimura ◽  
...  
Keyword(s):  
Rice Blast ◽  
Bacterial Blight ◽  
Lesion Mimic ◽  
Isolation And Characterization ◽  
Enhanced Resistance

Isolation and characterization of dominant mutations resistant to carbon catabolite repression of galactokinase synthesis in Saccharomyces cerevisiae

1981 ◽  
Vol 1 (2) ◽  
pp. 83-93
Author(s):  
K Matsumoto ◽  
A Toh-e ◽  
Y Oshima
Keyword(s):  
Partial Resistance ◽  
Carbon Catabolite Repression ◽  
Glucose Repression ◽  
Isocitrate Lyase ◽  
Isolation And Characterization ◽  
Enhanced Resistance ◽  
Leloir Pathway ◽  
Resistant Mutants ◽  
A Site

Seven dominant mutations showing greatly enhanced resistance to the glucose repression of galactokinase synthesis have been isolated from GAL81 mutants, which have the constitutive phenotype but are still strongly repressible by glucose for the synthesis of the Leloir enzymes. These glucose-resistant mutants were due to semidominant mutations at either of two loci, GAL82 and GAL83. Both loci are unlinked to the GAL81- gal4, gal80, or gal7 X gal10 X gal1 locus or to each other. The GAL83 locus was mapped on chromosome V at a site between arg9 and cho1. The GAL82 and GAL83 mutations produced partial resistance of galactokinase to glucose repression only when one or both of these mutations were combined with a GAL81 or a gal80 mutation. The GAL82 and GAL83 mutations are probably specific for expression of the Leloir pathway and related enzymes, because they do not affect the synthesis of alpha-D-glucosidase, invertase, or isocitrate lyase.

scholarly journals Isolation and Characterization of Rice OsHRL Gene Related to Bacterial Blight Resistance

2010 ◽  
Vol 26 (4) ◽  
pp. 417-420 ◽  
Author(s):  
Sang-Ryeol Park ◽  
Seok-Jun Moon ◽  
Dong-Jin Shin ◽  
Min-Gab Kim ◽  
Duk-Ju Hwang ◽  
...  
Keyword(s):  
Bacterial Blight ◽  
Blight Resistance ◽  
Bacterial Blight Resistance ◽  
Isolation And Characterization

Isolation and characterization of rice lesion mimic mutants from a T-DNA tagged population

2005 ◽  
Vol 15 (1) ◽  
pp. 17-23 ◽  
Author(s):  
Li Shutian ◽  
Pei Zhongyou ◽  
Luo Lijuan ◽  
Tian Yingchuan ◽  
He Chaozu
Keyword(s):  
Lesion Mimic ◽  
Isolation And Characterization

scholarly journals Isolation and Characterization of Avirulence Genes in Magnaporthe oryzae

2017 ◽  
Vol 7 (1) ◽  
pp. 31-42 ◽  
Author(s):  
Mui Sie Jee ◽  
Leonard Whye Kit Lim ◽  
Martina Azelin Dirum ◽  
Sara Ilia Che Hashim ◽  
Muhammad Shafiq Masri ◽  
...  
Keyword(s):  
Magnaporthe Oryzae ◽  
Fungal Pathogen ◽  
Rice Blast ◽  
Avirulence Gene ◽  
Avirulence Genes ◽  
Isolation And Characterization ◽  
Molecular Pattern ◽  
Avr Gene ◽  
Avr Genes

Magnaporthe oryzae is a fungal pathogen contributing to rice blast diseases globally via their Avr (avirulence) gene. Although the occurrence of M. oryzae has been reported in Sarawak since several decades ago, however, none has focused specifically on Avr genes, which confer resistance against pathogen associated molecular pattern-triggered immunity (PTI) in host. The objective of this study is to isolate Avr genes from M. oryzae 7’ (a Sarawak isolate) that may contribute to susceptibility of rice towards diseases. In this study, AvrPiz-t, AVR-Pik, Avr-Pi54, and AVR-Pita1 genes were isolated via PCR and cloning approaches. The genes were then compared with set of similar genes from related isolates derived from NCBI. Results revealed that all eight Avr genes (including four other global isolates) shared similar N-myristoylation site and a novel motif. 3D modeling revealed similar β-sandwich structure in AvrPiz-t and AVR-Pik despite sequence dissimilarities. In conclusion, it is confirmed of the presence of these genes in the Sarawak (M. oryzae) isolate. This study implies that Sarawak isolate may confer similar avirulence properties as their counterparts worldwide. Further R/Avr gene-for-gene relationship studies may aid in strategic control of rice blast diseases in future.

Isolation and characterization of Saccharomyces cerevisiae mutants with enhanced resistance to the anticancer drug bleomycin

2004 ◽  
Vol 45 (5) ◽  
pp. 265-272 ◽  
Author(s):  
Mustapha Aouida ◽  
Omar Tounekti ◽  
Anick Leduc ◽  
Omrane Belhadj ◽  
Lluis Mir ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Anticancer Drug ◽  
Isolation And Characterization ◽  
Enhanced Resistance

scholarly journals Characterizing Rice Lesion Mimic Mutants and Identifying a Mutant with Broad-Spectrum Resistance to Rice Blast and Bacterial Blight

2000 ◽  
Vol 13 (8) ◽  
pp. 869-876 ◽  
Author(s):  
Zhongchao Yin ◽  
Jun Chen ◽  
Lirong Zeng ◽  
Meiling Goh ◽  
Hei Leung ◽  
...  
Keyword(s):  
Cell Death ◽  
Disease Resistance ◽  
Broad Spectrum ◽  
Rice Blast ◽  
Bacterial Blight ◽  
Blue Staining ◽  
Lesion Mimic ◽  
Broad Spectrum Resistance ◽  
Defense Related Gene ◽  
Recessive Genes

Many plant mutants develop spontaneous lesions that resemble disease symptoms in the absence of pathogen attack. In several pathosystems, lesion mimic mutations have been shown to be involved in programmed cell death, which in some instances leads to enhanced disease resistance to multiple pathogens. We investigated the relationship between spontaneous cell death and disease resistance in rice with nine mutants with a range of lesion mimic phenotypes. All nine mutations are controlled by recessive genes and some of these mutants have stunted growth and other abnormal characteristics. The lesion mimics that appeared on the leaves of these mutants were caused by cell death as measured by trypan blue staining. Activation of six defense-related genes was observed in most of the mutants when the mimic lesions developed. Four mutants exhibited significant enhanced resistance to rice blast. One of the mutants, spl11, confers non-race-specific resistance not only to blast but also to bacterial blight. The level of resistance in the spl11 mutant to the two pathogens correlates with the defense-related gene expression and lesion development on the leaves. The results suggest that some lesion mimic mutations in rice may be involved in disease resistance, and cloning of these genes may provide a clue to developing broad-spectrum resistance to diverse pathogens.

scholarly journals Isolation and characterization of dominant mutations resistant to carbon catabolite repression of galactokinase synthesis in Saccharomyces cerevisiae.

1981 ◽  
Vol 1 (2) ◽  
pp. 83-93 ◽  
Author(s):  
K Matsumoto ◽  
A Toh-e ◽  
Y Oshima
Keyword(s):  
Partial Resistance ◽  
Carbon Catabolite Repression ◽  
Glucose Repression ◽  
Isocitrate Lyase ◽  
Isolation And Characterization ◽  
Enhanced Resistance ◽  
Leloir Pathway ◽  
Resistant Mutants ◽  
A Site

Seven dominant mutations showing greatly enhanced resistance to the glucose repression of galactokinase synthesis have been isolated from GAL81 mutants, which have the constitutive phenotype but are still strongly repressible by glucose for the synthesis of the Leloir enzymes. These glucose-resistant mutants were due to semidominant mutations at either of two loci, GAL82 and GAL83. Both loci are unlinked to the GAL81- gal4, gal80, or gal7 X gal10 X gal1 locus or to each other. The GAL83 locus was mapped on chromosome V at a site between arg9 and cho1. The GAL82 and GAL83 mutations produced partial resistance of galactokinase to glucose repression only when one or both of these mutations were combined with a GAL81 or a gal80 mutation. The GAL82 and GAL83 mutations are probably specific for expression of the Leloir pathway and related enzymes, because they do not affect the synthesis of alpha-D-glucosidase, invertase, or isocitrate lyase.

The isolation and characterization of 18 equine microsatellite loci, TKY272–TKY289

2000 ◽  
Vol 31 (2) ◽  
pp. 149-149 ◽  
Author(s):  
T Tozaki ◽  
H Kakoi ◽  
S Mashima ◽  
K Hirota ◽  
T Hasegawa ◽  
...  
Keyword(s):  
Microsatellite Loci ◽  
Isolation And Characterization
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