Somatic Embryogenesis and Plant Regeneration from Cultured Zygotic Embryos of Soybean (Glycine max L. Merr.)

1987 ◽  
Vol 128 (3) ◽  
pp. 219-226 ◽  
Author(s):  
N. Hammatt ◽  
M.R. Davey
2006 ◽  
Vol 49 (1) ◽  
pp. 49-55 ◽  
Author(s):  
Karla Galvão Bezerra dos Santos ◽  
Jorge Ernesto de Araújo Mariath ◽  
Maria Cecília C. Moço ◽  
Maria Helena Bodanese-Zanettini

Soybean somatic embryos obtained from immature zygotic embryos were histologically analyzed in order to determine possible ontogenetic routes followed by these embryos. The proembryo stage and globular, heart-shaped, torpedo and cotyledonary embryo stages were found, closely resembling the ontogeny of zygotic embryos. However, the absence of a characteristic suspensor as well as the delay in the establishment of inner organization were the main differences between these two embryogenic processes.


2002 ◽  
Vol 45 (3) ◽  
pp. 277-286 ◽  
Author(s):  
Juliana Aparecida Fernando ◽  
Maria Lúcia Carneiro Vieira ◽  
Isaías Olívio Geraldi ◽  
Beatriz Appezzato-da-Glória

A comparative anatomical analysis of somatic embryogenesis in two soybean (Glycine max (L.) Merrill) genotypes was carried out. The somatic embryos were originated from cotyledonary explants obtained from immature zygotic embryos. The medium used for somatic embryogenesis induction was Murashige and Skoog, 1962, salts and Gamborg et al., 1968, vitamins (MSB) supplemented with 0.8 mg.L-1 of 2,4-D for genotype PI 123439 and 40 mg.L-1 of 2,4-D for ‘Williams 82’. Globular structures, constituted by meristematic cells, originated from subepidermal cell divisions of the cotyledonary mesophyll. In PI 123439, the globular structures presented tracheary differentiation among meristematic cells and they could follow distinct morphogenetic process depending on their location along the explant. For ‘Williams 82’ it was observed globular structures along the cotyledonary explant surface. They gave rise to somatic embryos. These embryos showed different morphologies and they were classified based on their shape and number of cotyledons. The ability of these morphological types to convert to plantlets was discussed.


2012 ◽  
Vol 113 (3) ◽  
pp. 397-406 ◽  
Author(s):  
Suzana Pavlović ◽  
Branka Vinterhalter ◽  
Snežana Zdravković-Korać ◽  
Dragan Vinterhalter ◽  
Jasmina Zdravković ◽  
...  

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