Somatic embryogenesis and plant regeneration in Chinese soybean (Glycine max (L.) Merr.)—impacts of mannitol, abscisic acid, and explant age

2009 ◽  
Vol 45 (2) ◽  
pp. 180-188 ◽  
Author(s):  
Chao Yang ◽  
Tuanjie Zhao ◽  
Deyue Yu ◽  
Junyi Gai
Keyword(s):  
Somatic Embryogenesis ◽  
Abscisic Acid ◽  
Glycine Max ◽  
Plant Regeneration ◽  
Glycine Max L

Plant Regeneration from Cultured Medicago truncatula with Particular Reference to Abscisic Acid and Light Treatments

1998 ◽  
Vol 46 (1) ◽  
pp. 151 ◽  
Author(s):  
K. E. Nolan ◽  
R. J. Rose
Keyword(s):  
Acetic Acid ◽  
Somatic Embryogenesis ◽  
Abscisic Acid ◽  
Plant Regeneration ◽  
Medicago Truncatula ◽  
Callus Induction ◽  
Naphthalene Acetic Acid ◽  
Marked Inhibition ◽  
Embryo Formation ◽  
Auxin Concentration

Medicago truncatula (Jemalong 2HA) can be regenerated by somatic embryogenesis utilising 1-naphthalene acetic acid (NAA) and 6-benzylaminopurine (BAP). There is a requirement for both NAA and BAP for callus induction and embryo formation. There is no requirement for a drop in auxin concentration to induce embryos. Abscisic acid (ABA) when present with NAA and BAP during embryo formation at a concentration of 1 µM, increases the number of embryos per callus. The ABA treatment stimulates embryo numbers in both light and darkness. The conversion efficiency of embryo to plant is unchanged irrespective of the presence of ABA during embryo formation, indicating that ABA does not improve the regeneration of the embryos once formed. Importantly, the presence of light in the embryo formation period causes a marked inhibition of embryo conversion.

Organogenic callus as the target for plant regeneration and transformation via Agrobacterium in soybean (Glycine max (L.) Merr.)

2007 ◽  
Vol 43 (6) ◽  
pp. 558-568 ◽  
Author(s):  
Hai Ping Hong ◽  
Hongyi Zhang ◽  
Paula Olhoft ◽  
Steve Hill ◽  
Hunt Wiley ◽  
...  
Keyword(s):  
Glycine Max ◽  
Plant Regeneration ◽  
Organogenic Callus ◽  
Glycine Max L

Regeneration of soybean (Glycine max L. Merrill) through direct somatic embryogenesis from the immature embryonic shoot tip

2010 ◽  
Vol 46 (3) ◽  
pp. 265-273 ◽  
Author(s):  
Murugan Loganathan ◽  
Subbiyan Maruthasalam ◽  
Ling Yin Shiu ◽  
Wei Ching Lien ◽  
Wen Hwei Hsu ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Glycine Max ◽  
Shoot Tip ◽  
Direct Somatic Embryogenesis ◽  
Glycine Max L
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