Template properties of ultraviolet-irradiated poly(dC) replicated by E. coli DNA polymerase I: Indication for a role of apyrimidinic-sites in UV-induced mutagenesis

Biochimie ◽  
1985 ◽  
Vol 67 (3-4) ◽  
pp. 371-376 ◽  
Author(s):  
Serge Boiteux ◽  
Josiane Pierre ◽  
Jacques Laval
Keyword(s):  
Dna Polymerase ◽  
Dna Polymerase I ◽  
E Coli ◽  
Induced Mutagenesis ◽  
Polymerase I

THE INFLUENCE OF THE PO1A1 MUTATION UPON RECOMBINATION IN ESCHERICHIA COLI K12

1979 ◽  
Vol 21 (3) ◽  
pp. 423-428 ◽  
Author(s):  
Barry W. Glickman ◽  
Tineke Rutgers
Keyword(s):  
Escherichia Coli ◽  
Dna Polymerase ◽  
Genetic Recombination ◽  
Dna Polymerase I ◽  
Multiple Gene ◽  
E Coli ◽  
Recombinational Process ◽  
K 12 ◽  
Polymerase I

Genetic recombination in Escherichia coli is a highly regulated process involving multiple gene products. We have investigated the role of DNA polymerase I in this process by studying the effect of the po1A1 mutation upon DNA transfer and conjugation in otherwise isogenic suppressor-free strains of E. coli K-12. It was found that the po1A1 mutation greatly reduces recombination in Hfr crosses (a factor of 20 in Po1+ × Po1A1 crosses and more than a factor of 100 in Po1A1 × Po1A1 crosses). However, since the po1A1 mutation reduces the strains capacity to act as a recipient for an F-prime and the analysis of recombination transfer gradients revealed no differences between Po1+ and Po1− strains, it is concluded that DNA polymerase I probably affects the transfer and/or stability of donor DNA rather than the recombinational process itself.

Site-specific modification of E. coli DNA polymerase I large fragment with pyridoxal 5'-phosphate

Biochemistry ◽  
1984 ◽  
Vol 23 (9) ◽  
pp. 2073-2078 ◽  
Author(s):  
Anup K. Hazra ◽  
Sevilla Detera-Wadleigh ◽  
Samuel H. Wilson
Keyword(s):  
Dna Polymerase ◽  
Large Fragment ◽  
Dna Polymerase I ◽  
Site Specific ◽  
E Coli ◽  
Specific Modification ◽  
Polymerase I

scholarly journals Genetic evidence for two protein domains and a potential new activity in bacteriophage T4 DNA polymerase.

Genetics ◽  
1990 ◽  
Vol 124 (2) ◽  
pp. 213-220 ◽  
Author(s):  
L J Reha-Krantz
Keyword(s):  
Dna Polymerase ◽  
Bacteriophage T4 ◽  
Rnase H ◽  
Ribonuclease H ◽  
Temperature Sensitive ◽  
Polymerase Gene ◽  
Dna Polymerase I ◽  
E Coli ◽  
Intragenic Complementation ◽  
Polymerase I

Abstract Intragenic complementation was detected within the bacteriophage T4 DNA polymerase gene. Complementation was observed between specific amino (N)-terminal, temperature-sensitive (ts) mutator mutants and more carboxy (C)-terminal mutants lacking DNA polymerase polymerizing functions. Protein sequences surrounding N-terminal mutation sites are similar to sequences found in Escherichia coli ribonuclease H (RNase H) and in the 5'----3' exonuclease domain of E. coli DNA polymerase I. These observations suggest that T4 DNA polymerase, like E. coli DNA polymerase I, contains a discrete N-terminal domain.

How E. coli DNA polymerase I (klenow fragment) distinguishes between deoxy- and dideoxynucleotides

1998 ◽  
Vol 278 (1) ◽  
pp. 147-165 ◽  
Author(s):  
Mekbib Astatke ◽  
Nigel D.F Grindley ◽  
Catherine M Joyce
Keyword(s):  
Dna Polymerase ◽  
Klenow Fragment ◽  
Dna Polymerase I ◽  
E Coli ◽  
Polymerase I

Inhibition of E. coli DNA polymerase I by 1,10-phenanthroline

1977 ◽  
Vol 78 (1) ◽  
pp. 170-176 ◽  
Author(s):  
Vito D'Aurora ◽  
Andrew M. Stern ◽  
David S. Sigman
Keyword(s):  
Dna Polymerase ◽  
Dna Polymerase I ◽  
E Coli ◽  
Polymerase I
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