scholarly journals Assignment of the mouse desmin gene to chromosome 1 band C3

1990 ◽  
Vol 55 (2) ◽  
pp. 101-105 ◽  
Author(s):  
Zhenlin Li ◽  
Marie-Geneviève Mattei ◽  
Jean-François Mattei ◽  
Denise Paulin

SummaryThe chromosomal localization of the mouse gene coding for desmin, one of the muscle-specific intermediate filament subunits, was determined by in situ hybridization using a specific 3H-labelled DNA probe. There is only one copy of the desmin gene and it is located on chromosome 1 in the band C3. This result adds an eleventh locus to a conserved gene cluster and confirms the partial homology that exists between the long arm of human chromosome 2 and chromosome 1 of the mouse.

1989 ◽  
Vol 53 (3) ◽  
pp. 183-185 ◽  
Author(s):  
Marie-Genevieve Mattei ◽  
Alain Lilienbaum ◽  
Li Zhen Lin ◽  
Jean-François Mattei ◽  
Denise Paulin

SummaryThe chromosomal location of the mouse gene coding for vimentin, one of the intermediate filament subunits, was determined by in situ hybridization using specific H3-labelled DNA probes. There is only one copy of the vimentin gene and it is located on chromosome 2 region A2.


1997 ◽  
Vol 79 (1-2) ◽  
pp. 143-144 ◽  
Author(s):  
J. Aurich-Costa ◽  
S. Cadel ◽  
C. Gouzy ◽  
T. Foulon ◽  
D. Chérif ◽  
...  

Genome ◽  
1993 ◽  
Vol 36 (3) ◽  
pp. 517-523 ◽  
Author(s):  
I. J. Leitch ◽  
J. S. Heslop-Harrison

The 5S rDNA sequences have been mapped on four pairs of barley (Hordeum vulgare L.) chromosomes using in situ hybridization and barley monotelotrisomic lines. The 5S rDNA sequences are located, genetically and physically, on the short arm of chromosome 1 (7I) and the long arms of chromosomes 2 (2I) and 3 (3I). The 5S rDNA sequence is also located on the physically long arm of chromosome 4 (4I). Only one site on chromosome 2(2I) has previously been reported. The characteristic locations of the 5S rDNA sequences make them useful as molecular markers to identify each barley chromosome. The physical position of the low-copy α-amylase-2 gene was determined using in situ hybridization; the location of this gene on the long arm of chromosome 1 (7I) was confirmed by reprobing the same preparation with the 5S rDNA probe. The results show that there is a discrepancy between the physical and genetic position of the α-amylase-2 gene.Key words: genetic mapping, physical mapping, barley, mapping, 5S DNA, α-amylase, in situ hybridization.


1977 ◽  
Vol 36 (1) ◽  
pp. 25-33 ◽  
Author(s):  
Dale M. Steffensen ◽  
Ernest H. Y. Chu ◽  
David P. Speert ◽  
Patrick M. Wall ◽  
Karen Meilinger ◽  
...  

Genetics ◽  
2002 ◽  
Vol 162 (3) ◽  
pp. 1415-1422 ◽  
Author(s):  
E Sanchez-Moran ◽  
S J Armstrong ◽  
J L Santos ◽  
F C H Franklin ◽  
G H Jones

Abstract Natural variation in meiotic recombination frequency in Arabidopsis thaliana has been assessed by analyzing chiasma frequency variation among a range of geographically and ecologically diverse accessions. Fifty pollen mother cells at metaphase I of meiosis were analyzed from each of eight accessions and fluorescence in situ hybridization was applied to enable identification of all 10 chromosome arms. There was no significant variation in mean chiasma frequency between plants within accessions, but there was significant variation between accessions. Further analysis confirmed this finding and identified two particular accessions, Cvi and Ler, as having chiasma frequencies significantly lower than those of the other accessions. The analysis also revealed that the pattern of chiasma distribution between arms and among chromosomes is not consistent over accessions. Further detailed analyses were conducted on each individual chromosome (1-5) in turn, revealing that chromosome 4, one of the acrocentric chromosomes, is the least variable while the other acrocentric chromosome (2) is the most variable. These findings indicate the existence of recombination regulatory elements in Arabidopsis and we conclude that it may be possible in the future to identify these elements and determine their mode of action. The practical implications of such developments are considerable.


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