scholarly journals Chromosomal localization of the mouse gene coding for vimentin

1989 ◽  
Vol 53 (3) ◽  
pp. 183-185 ◽  
Author(s):  
Marie-Genevieve Mattei ◽  
Alain Lilienbaum ◽  
Li Zhen Lin ◽  
Jean-François Mattei ◽  
Denise Paulin
Keyword(s):  
Intermediate Filament ◽  
Chromosomal Localization ◽  
Chromosomal Location ◽  
Dna Probes ◽  
Mouse Gene ◽  
Chromosome 2 ◽  
Gene Coding ◽  
Vimentin Gene

SummaryThe chromosomal location of the mouse gene coding for vimentin, one of the intermediate filament subunits, was determined by in situ hybridization using specific H3-labelled DNA probes. There is only one copy of the vimentin gene and it is located on chromosome 2 region A2.

scholarly journals Assignment of the mouse desmin gene to chromosome 1 band C3

1990 ◽  
Vol 55 (2) ◽  
pp. 101-105 ◽  
Author(s):  
Zhenlin Li ◽  
Marie-Geneviève Mattei ◽  
Jean-François Mattei ◽  
Denise Paulin
Keyword(s):  
Human Chromosome ◽  
Gene Cluster ◽  
Intermediate Filament ◽  
Chromosomal Localization ◽  
Chromosome 1 ◽  
Chromosome 2 ◽  
Gene Coding ◽  
Partial Homology ◽  
Conserved Gene

SummaryThe chromosomal localization of the mouse gene coding for desmin, one of the muscle-specific intermediate filament subunits, was determined by in situ hybridization using a specific 3H-labelled DNA probe. There is only one copy of the desmin gene and it is located on chromosome 1 in the band C3. This result adds an eleventh locus to a conserved gene cluster and confirms the partial homology that exists between the long arm of human chromosome 2 and chromosome 1 of the mouse.

Cloning, characterization, and chromosomal localization of human liver form cytochrome c oxidase subunit VIa related genes

Genome ◽  
1997 ◽  
Vol 40 (3) ◽  
pp. 325-331 ◽  
Author(s):  
Frank Merante ◽  
Mingfu Ling ◽  
Catherine Duff ◽  
Brian H. Robinson ◽  
Alessandra M. V. Duncan
Keyword(s):  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Human Liver ◽  
Somatic Cell Hybrid ◽  
Chromosomal Localization ◽  
Chromosomal Location ◽  
Chromosome 6 ◽  
Human Cytochrome ◽  
Human Cytochrome C

The chromosomal location of human cytochrome c oxidase (COX) subunit VIa Liver (VIa-L) isoform related sequences has been determined by a combination of in situ hybridization and analysis of human–hamster somatic cell hybrid panels. COX VIa-L related sequences were present on chromosomes 6 and 12. It has been verified that at least two COX VIa-L genes are on chromosome 6, one of which is a pseudogene. In total, four COX VIa-L related sequences have been cloned and their nucleotide sequences analyzed. At least three of these sequences represent pseudogenes; their relatedness to the COX VIa-L cDNA is discussed.Key words: human, cytochrome c oxidase, chromosomal localization, COX VIa, cloning.

In situ localization of a mammalian protamine gene: parameters affecting specificity of hybridization

Genome ◽  
1990 ◽  
Vol 33 (3) ◽  
pp. 459-463 ◽  
Author(s):  
Stephen A. Krawetz ◽  
Manfred H. Herfort ◽  
Gordon H. Dixon
Keyword(s):  
Genomic Dna ◽  
Southern Hybridization ◽  
Chromosomal Localization ◽  
Chromosomal Location ◽  
Cdna Probe ◽  
Chromosome 1 ◽  
Telomeric Region ◽  
Southern Hybridization Analysis ◽  
Indian Muntjac

Southern hybridization analysis of Indian muntjac genomic DNA with the Eco-Taq bovine genomic protamine probe revealed a simple banding pattern. The pattern of hybridization was identical with that previously observed in the genus Bos. This suggested that the bovine probe specifically hybridized to the Indian muntjac protamine gene. The opportunity was thus provided to assign the chromosomal location of the protamine gene in a comparatively simple system. Accordingly, this probe and the corresponding cDNA probe were used for in situ chromosome hybridization and localization. Various parameters affecting specificity and the resolution of hybridization were examined. Subsequent to optimization, the Indian muntjac gene was shown to be autosomal and distally located in the telomeric region of the p arm of chromosome 1.Key words: chromosomal localization, muntjac, protamine, specificity, hybridization.

Chromosomal localization of the mouse gene coding for the 68 kDa neurofilament subunit

1989 ◽  
Vol 67 (2) ◽  
pp. 235-237 ◽  
Author(s):  
Marie-Geneviève Matte ◽  
Philippe Duprey ◽  
Zhen Lin Li ◽  
Jean-François Mattei ◽  
Denise Paulin
Keyword(s):  
Chromosomal Localization ◽  
Mouse Gene ◽  
Gene Coding

scholarly journals New Data on Organization and Spatial Localization of B-Chromosomes in Cell Nuclei of the Yellow-Necked Mouse Apodemus flavicollis

Cells ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1819
Author(s):  
Tatyana Karamysheva ◽  
Svetlana Romanenko ◽  
Alexey Makunin ◽  
Marija Rajičić ◽  
Alexey Bogdanov ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Sex Chromosomes ◽  
Interphase Nucleus ◽  
Spatial Organization ◽  
Three Dimensional ◽  
Dna Probes ◽  
B Chromosomes ◽  
Apodemus Flavicollis ◽  
Yellow Necked Mouse

The gene composition, function and evolution of B-chromosomes (Bs) have been actively discussed in recent years. However, the additional genomic elements are still enigmatic. One of Bs mysteries is their spatial organization in the interphase nucleus. It is known that heterochromatic compartments are not randomly localized in a nucleus. The purpose of this work was to study the organization and three-dimensional spatial arrangement of Bs in the interphase nucleus. Using microdissection of Bs and autosome centromeric heterochromatic regions of the yellow-necked mouse (Apodemus flavicollis) we obtained DNA probes for further two-dimensional (2D)- and three-dimensional (3D)- fluorescence in situ hybridization (FISH) studies. Simultaneous in situ hybridization of obtained here B-specific DNA probes and autosomal C-positive pericentromeric region-specific probes further corroborated the previously stated hypothesis about the pseudoautosomal origin of the additional chromosomes of this species. Analysis of the spatial organization of the Bs demonstrated the peripheral location of B-specific chromatin within the interphase nucleus and feasible contact with the nuclear envelope (similarly to pericentromeric regions of autosomes and sex chromosomes). It is assumed that such interaction is essential for the regulation of nuclear architecture. It also points out that Bs may follow the same mechanism as sex chromosomes to avoid a meiotic checkpoint.

INHERITANCE AND LINKAGE STUDIES WITH THE GRANDPA GENE IN BARLEY, HORDEUM VULGARE L.

1971 ◽  
Vol 13 (3) ◽  
pp. 489-498
Author(s):  
R. W. Matchett ◽  
H. G. Nass ◽  
D. W. Robertson
Keyword(s):  
Hordeum Vulgare ◽  
Chromosomal Location ◽  
Gene Interactions ◽  
Chromosome 2 ◽  
Linkage Studies ◽  
Hordeum Vulgare L ◽  
Barley Genome ◽  
Chlorophyll Mutants ◽  
Mutant Genes ◽  
Linkage Association

This study was initiated to determine the chromosomal location of the grandpa (gp) gene within the barley genome. The gp gene was placed on the long arm of chromosome 2 as indicated by linkage association with liguleless (li).Tests of allelism showed the gp gene to the allelic with the gp-2 gene. Seven sources of "yellow" chlorophyll mutants when crossed to grandpa plants gave albino double recessive seedlings. Three other sources of "yellow" chlorophyll mutants in the double recessive combination with grandpa exhibited yellow and white bands on the leaves. Double recessive individuals carrying the mottled (mt2) and grandpa genes were also albino. This is evidence of gene interactions between chlorophyll mutant genes.

scholarly journals In situ hybridisation with digoxigenin-labelled DNA probes for detection of viral genomes.

1990 ◽  
Vol 43 (10) ◽  
pp. 806-809 ◽  
Author(s):  
Y Furuta ◽  
T Shinohara ◽  
K Sano ◽  
M Meguro ◽  
K Nagashima
Keyword(s):  
In Situ Hybridisation ◽  
Dna Probes ◽  
Viral Genomes

scholarly journals Improved In Situ Hybridization Efficiency with Locked-Nucleic-Acid-Incorporated DNA Probes

2006 ◽  
Vol 72 (8) ◽  
pp. 5311-5317 ◽  
Author(s):  
Kengo Kubota ◽  
Akiyoshi Ohashi ◽  
Hiroyuki Imachi ◽  
Hideki Harada
Keyword(s):  
In Situ Hybridization ◽  
Nucleic Acid ◽  
Fluorescence Intensity ◽  
Dna Probes ◽  
Locked Nucleic Acid ◽  
Factors Affecting ◽  
Hybridization Efficiency ◽  
Probe Hybridization ◽  
Major Factors

ABSTRACT Low signal intensity due to poor probe hybridization efficiency is one of the major drawbacks of rRNA-targeted in situ hybridization. There are two major factors affecting the hybridization efficiency: probe accessibility and affinity to the targeted rRNA molecules. In this study, we demonstrate remarkable improvement in in situ hybridization efficiency by applying locked-nucleic-acid (LNA)-incorporated oligodeoxynucleotide probes (LNA/DNA probes) without compromising specificity. Fluorescently labeled LNA/DNA probes with two to four LNA substitutions exhibited strong fluorescence intensities equal to or greater than that of probe Eub338, although these probes did not show bright signals when they were synthesized as DNA probes; for example, the fluorescence intensity of probe Eco468 increased by 22-fold after three LNA bases were substituted for DNA bases. Dissociation profiles of the probes revealed that the dissociation temperature was directly related to the number of LNA substitutions and the fluorescence intensity. These results suggest that the introduction of LNA residues in DNA probes will be a useful approach for effectively enhancing probe hybridization efficiency.

Identification of the origin of centromeres in whole-arm translocations using fluorescent in situ hybridization with α-satellite DNA probes

1991 ◽  
Vol 40 (1) ◽  
pp. 117-120 ◽  
Author(s):  
Avirachan T. Tharapel ◽  
Mazin B. Qumsiyeh ◽  
Paula R. Martens ◽  
Sugandhi A. Tharapel ◽  
James D. Dalton ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Satellite Dna ◽  
Fluorescent In Situ Hybridization ◽  
Dna Probes
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