Nuclear Sm antigens in the sperm of different organisms

Zygote ◽  
1994 ◽  
Vol 2 (3) ◽  
pp. 227-235 ◽  
Author(s):  
Fernando Delgadoa ◽  
Monica Brito ◽  
Ilona I. Concha ◽  
Ricardo Schroeder ◽  
Luis O. Burzio
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Cyprinus Carpio ◽  
Hela Cells ◽  
Lupus Erythematosus ◽  
Teleost Fish ◽  
Immunoblot Analysis ◽  
Sperm Nucleus ◽  
Systemic Lupus ◽  
Sperm Protein ◽  
Polypeptide Profile

SummaryImmunoblot analysis of sperm protein from several species revealed the presence of polypeptides recognised by anti-Sm sera obtained from patients with systemic lupus erythematosus. Immunoreactive polypeptides in human, bull, mouse and rat sperm were identified as protein B', B and D as compared with the Sm polypeptides of HeLa cells. In the sperm of rooster, the teleost fish Cyprinus carpio and the mussel Choromytilus chorus, the immunoreactive polypeptide profile was more complex. To ascertain the sperm origin of the Sm antigens, immunolocalisation with anti-Sm serum was carried out. The results demonstrated that in all the specied studied staining was confinde to the sperm nucleus, confirming that some polypeptides of the small nuclear ribonnucleoprotein complex are present in the gamete.

False positive seroreactivity to Borrelia burgdorferi in systemic lupus erythematosus: the value of immunoblot analysis

Lupus ◽  
1995 ◽  
Vol 4 (2) ◽  
pp. 131-137 ◽  
Author(s):  
Nancy L Weiss ◽  
Victoria A Sadock ◽  
Leonard H Sigal ◽  
Mark Phillips ◽  
Parvin F Merryman ◽  
...  
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Borrelia Burgdorferi ◽  
Lupus Erythematosus ◽  
False Positive ◽  
Immunoblot Analysis ◽  
Systemic Lupus

scholarly journals Interleukin 6 Gene Polymorphisms Are Associated with Systemic Lupus Erythematosus in Koreans

2010 ◽  
Vol 37 (11) ◽  
pp. 2251-2258 ◽  
Author(s):  
JA-YOUNG JEON ◽  
HYOUN-AH KIM ◽  
SEUNG-HYUN KIM ◽  
HAE-SIM PARK ◽  
CHANG-HEE SUH
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Hela Cells ◽  
Lupus Erythematosus ◽  
Interleukin 6 ◽  
Gene Polymorphisms ◽  
Promoter Activity ◽  
Luciferase Reporter ◽  
Systemic Lupus ◽  
Exon 2 ◽  
Hep3b Cells

Objective.Interleukin 6 (IL-6) gene polymorphisms are known to play a role in chronic inflammatory disorders. We searched for polymorphisms in theIL-6gene and described their pathogenic role in Korean patients with systemic lupus erythematosus (SLE).Methods.Genomic DNA was extracted from 151 patients with SLE and 151 controls, and about 1.4 kb-sizedIL-6genes located between promoter region and exon 2 region were amplified by polymerase chain reaction. The promoter activity was analyzed by luciferase reporter assay in Hep3B cells and HeLa cells.Results.We identified 4 single-nucleotide polymorphisms (SNP; −572 C > G, −278 A > C in the promoter, and 330 T > G, and 334 A > T in exon 2) and a −373 AnTntract polymorphism in theIL-6gene. The genotype frequency, −373 A10T11, −278 C, and 334 T allele were significantly associated with SLE (p < 0.001, p = 0.03 and p = 0.005, respectively). Patients with SLE carrying the −572 G allele had anti-dsDNA more frequently (p = 0.007). In addition, thrombocytopenia was significantly more common in patients carrying the −278 C allele (p = 0.006). In the haplotype analysis, patients with SLE had more frequently haplotype HT3 (CA10T11ATA, dominant model, p = 0.012) that was associated with arthritis, leukopenia, anti-dsDNA, and hypocomplementemia. Promoter reporter structures carrying the −278 C allele displayed significantly higher promoter activity than the −278 A allele in Hep3B cells (p < 0.001) and HeLa cells (p < 0.001).Conclusion.These data suggest thatIL-6gene polymorphisms are associated with disease susceptibility and phenotype of SLE. In addition, promoter polymorphisms may be involved in regulation ofIL-6expression.

Organized Intraglomerular Deposits in NZB Mouse Disease

1971 ◽  
Vol 29 ◽  
pp. 544-545
Author(s):  
Francis R. Comerford ◽  
Alan S. Cohen
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Lupus Erythematosus ◽  
Antinuclear Antibodies ◽  
Human Systemic Lupus Erythematosus ◽  
Systemic Lupus ◽  
Glomerular Lesion ◽  
Ultrastructural Studies ◽  
Dense Deposits ◽  
Experimental Nephritis ◽  
Glomerular Lesions

Mice of the inbred NZB strain develop a spontaneous disease characterized by autoimmune hemolytic anemia, positive lupus erythematosus cell tests and antinuclear antibodies and nephritis. This disease is analogous to human systemic lupus erythematosus. In ultrastructural studies of the glomerular lesion in NZB mice, intraglomerular dense deposits in mesangial, subepithelial and subendothelial locations were described. In common with the findings in many examples of human and experimental nephritis, including many cases of human lupus nephritis, these deposits were amorphous or slightly granular in appearance with no definable substructure.We have recently observed structured deposits in the glomeruli of NZB mice. They were uncommon and were found in older animals with severe glomerular lesions by morphologic criteria. They were seen most commonly as extracellular elements in subendothelial and mesangial regions. The deposits ranged up to 3 microns in greatest dimension and were often adjacent to deposits of lipid-like round particles of 30 to 250 millimicrons in diameter and with amorphous dense deposits.

“Subcortical” cognitive impairment in patients with systemic lupus erythematosus

2000 ◽  
Vol 6 (7) ◽  
pp. 821-825 ◽  
Author(s):  
ELIZABETH LERITZ ◽  
JASON BRANDT ◽  
MELISSA MINOR ◽  
FRANCES REIS-JENSEN ◽  
MICHELLE PETRI
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Cognitive Impairment ◽  
Lupus Erythematosus ◽  
Systemic Lupus
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