Identification of amino acids from air shade dried powered material of Citrus limon L Burmf (Assam lemon) leaves was carried out in this experiment. Paper chromatographic technique was used for the qualitative determination of amino acids with different mobile phases. Specific amino acids were detected in specific mobile phases. The obtained amino acids were identified by comparing with standard amino acids. Total nine amino acids were detected from of Citrus limon L Burmf leaves. Two mobile phases were found suitable for detection of amino acids present in the leaves of citrus limon L Burmf.
Keyword: Citrus limon, Paper chromatography, Amino acids.