Design of an Integrated Microfluidic Paper-Based Chip and Inspection Machine for the Detection of Mercury in Food with Silver Nanoparticles

For most of the fast screening test papers for detecting Hg2+, the obtained results are qualitative. This study developed an operation for the μPAD and combined it with the chemical colorimetric method. Silver nanoparticle (AgNP) colloids were adopted as the reactive color reagent to combine and react with the Hg standards on the paper-based chip. Then, the RGB values for the color change were used to establish the standard curve (R2 > 0.99). Subsequently, this detection system was employed for the detection tests of actual samples, and the detected RGB values of the samples were substituted back to the formula to calculate the Hg2+ contents in the food. In this study, the Hg2+ content and recovery rate in commercially available packaged water and edible salts were measured. The research results indicate that a swift, economical, and simple detection method for Hg2+ content in food has been successfully developed.

Correlation of Phenolic Content of Multiflora Honey from Malang and Alastuwo to Activity Antioxidant Using DPPH (2.2-Diphenyl-1-Pickrylhydrazyl)

Honey contains various compounds that function as antioxidants, one of which is phenolic compounds. The purpose of this study was to determine the correlation between the phenolic content of multiflora honey from Malang and Alastuwo on antioxidant activity using the DPPH method (2,2-Diphenyl-1-picrylhydrazyl). Qualitative test of phenolic compounds with FeCl3 color reagent, determination of total phenolic content by UV-Vis spectrophotometry using Folin-Ciocalteu method and antioxidant activity test using DPPH method (2,2-Diphenyl-1-picrylhydrazyl). The results showed that qualitatively Malang and Alastuwo honey contained phenolic compounds. The total phenolic content of Alastuwo honey was 0.0278 ± 0.0010 mg GAE/g and Malang honey was 0.0301 ± 0.0004 mg GAE/g. The results of the antioxidant activity test showed that Alastuwo honey had weak antioxidant activity (IC50 of 393.37 ± 10.28 ppm), Malang honey had moderate antioxidant activity (IC50 of 217.20 ± 6.61 ppm) and vitamin C had strong antioxidant activity. (IC50 is 2.22 ± 0.19 ppm). The total phenolic content of Alastuwo and Malang honey correlates with its antioxidant activity.

Profil Fitokimia Ekstrak Etil Asetat Temu Kunci (Boesenbergia rotunda L.) dan Serai (Cymbopogon citratus)

Senyawa bahan alam yang terdapat pada tanaman memiliki banyak khasiat bagi kesehatan yang telah dibuktikan melalui pengobatan tradisional secara empiris. Identifikasi senyawa kimia sangat penting untuk mengetahui kemungkinan adanya senyawa yang dapat memiliki aktivitas farmakologi. Tanaman yang telah banyak digunakan oleh masyarakat termasuk pengobatan adalah temu kunci (Boesenbergia rotunda L.) dan serai (Cymbopogon citratus). Temu kunci dan serai diekstraksi menggunakan pelarut etil asetat. Uji fitokimia pada ekstrak etil asetat temu kunci dan serai dilakukan dengan uji kualitatif pereaksi warna dan pengendapan serta kromatografi lapis tipis untuk melihat gambaran pemisahan senyawa kimia yang terkandung. Ekstrak etil asetat temu kunci dan serai mengandung senyawa golongan alkaloid, terpenoid, flavonoid, fenol, dan kuinon. Senyawa pada temu kunci dan serai dapat dipisahkan menggunakan kromatografi lapis tipis dengan fase gerak etil asetat : n-heksan (8:2). Kata Kunci: Fitokimia, Temu Kunci, Serai, Ekstrak Etil Asetat, Kromatografi Lapis Tipis Natural compounds found in plants have many health benefits that have been proven through empirically traditional medicine. Identification of chemical compounds is very important to determine the possibility of compounds having pharmacological activity. Plants that have been widely used by the community, including medicinal plants, are Temu Kunci (Boesenbergia rotunda L.) and serai or lemongrass (Cymbopogon citratus). Temu Kunci and lemongrass were extracted using ethyl acetate as a solvent. Phytochemical test on ethyl acetate extract of temu Kunci and lemongrass was carried out by qualitative test using color reagent and deposition and thin layer chromatography to see the description of the separation of the chemical compounds contained. Temu Kunci and lemongrass ethyl acetate extracts contain alkaloids, terpenoids, flavonoids, phenols, and quinones. Compounds in Temu Kunci and lemongrass can be separated using thin layer chromatography with ethyl acetate: n-hexane (8: 2) as mobile phase.

Identifikasi Senyawa Metabolit Sekunder pada Ekstrak Daun Gayam (Inocarpus Fagifer Fosb) dengan Menggunakan KLT

The Gayam tree is widespread in the territory of Indonesia, the Gayam plant (Inocarfus Fegifer Fosb) is also used as a medicine for bloody bowel movements in the community by boiling the stems and drinking boiled water. Besides having medicinal properties, Gayam is also used as food in the community in the Banjarmasin area. The common people certainly do not know the benefits of gayam and its nutritional value. Research on the identification of secondary metabolite compounds in Gayam leaf extract (Inocarpus Fagifer Fosb) using TLC where Gayam Leaves (Inocarpus Fagifer Fosb) was extracted by maceration method, the thick extract obtained was then carried out by testing the identification of secondary metabolites with color reagent and TLC. The results of the identification of the compound metabolite of gayam leaves (Inocarfus Fegifer Fosb) by color reagent and TLC test showed positive test results for alkaloids, flavonoids, terpenoids, saponins.

NEW, GREEN AND MINIATURIZED ANALYTICAL METHOD FOR DETERMINATION OF CEFADROXIL MONOHYDRATE IN CAPSULES

Cefadroxil, an oral antimicrobial, presents few techniques optimized for the reduction of solvents and toxic residues and/or non-use of them. So, a quantitative, new and miniaturized method for determination of cefadroxil monohydrate in capsules has been developed and validated by spectrophotometric method in the visible region according to the international guidelines. The analyzes were performed using microplates containing 96 wells, 1 % of phenolphthalein and sodium hydroxide 0.1 M as reagent at 552 nm. The method was (i) linear in the range of 15-115 µg mL-1, (ii) selective when comparing standard, sample, adjuvants and color reagent, (iii) precise with deviations below 4 %, (iv) accurate when comparing the proposed method with the HPLC method, (v) robusts by making small and deliberate modifications to the method, (vi) besides being fast, low cost, eco-friendly and generates minimal amount of waste. The method can be applied to the routine quality control of cefadroxil monohydrate in capsules and an effective and accessible alternative that contemplates the concepts of current and sustainable green analytical chemistry.

ANALISIS BORAKS PADA ABU KOPRA DI MINAHASA UTARA DAN MINAHASA TENGGARA

ABSTRACT Copra ashes are ash produced from burning coconut husk in the copra roasting process after more than three roasts. Noodle producers in Manado and Tombatu were using copra ashes as an additive to noodles because it has benefits as a substitute for borax, which is prohibited from using on food, since it can improve the texture of noodles, as preservatives, improve the color and appearance of noodles. The purpose of this study is to analyze qualitatively and quantitatively the presence of borax compounds in copra ashes. Qualitatively analysis was carried out using the flame and color method, and the quantitative test used the UV-Vis spectrophotometry method at the wavelength of 545 nm and 0.125% curcumin as the color reagent. The study showed positive result on the qualitative test of the color method against turmeric paper whereas turn to brownish red, and negative result on the flame test whereas turn to red greenish fire, on the quantitative test the sample level A=1.459 ppm and sample B=2.092 this indicates that samples A and B were positive contains borax.Keywords: Copra ashes, food additives, borax and spectrophotometry ABSTRAK Abu kopra adalah abu yang dihasilkan dari pembakaran sabut kelapa dalam proses pemanggangan kopra setelah lebih dari 3 kali pemanggangan. Abu kopra oleh produsen mie di Airmadidi dan di Tombatu digunakan sebagai bahan tambahan pada mie karena memiliki manfaat sebagai pengganti boraks yang dilarang penggunaannya pada makanan, karena dapat memperbaiki tekstur mie, sebagai pengawet dan memperbaiki warna dan penampilan mie. Tujuan penelitian ini yaitu menganalisis secara kualitatif dan kuantitatif keberadaan senyawa boraks di dalam abu kopra. Analisis kualitatif dilakukan dengan metode nyala dan warna, dan uji kuantitatif digunakan metode spektrofometri UV-Vis pada panjang gelombang 545 nm dan kurkumin 0,125% sebagai pereaksi warnanya. Penelitian menunjukkan hasil positif pada uji kualitatif metode warna yaitu kertas tumerik menjadi merah kecoklatan, dan hasil negatif pada uji nyala yaitu api berwarna merah kehijauan, pada uji kuantitatif kadar sampel A=1,459 ppm dan sampel B=2,092 ini menunjukkan bahwa sampel A dan B positif mengandung boraks. Kata kunci : Abu kopra, bahan tambahan makanan, boraks dan spektrofotometri.

PERBANDINGAN KADAR PROTEIN TELUR PADA TELUR AYAM DENGAN METODE SPEKTROFOTOMETRI VIS

<p align="center"><strong>Abstrak</strong></p><p align="center"><strong> </strong>Protein yang terdapat dalam makanan hewani seperti telur dikatakan sebagai protein sempurna. Telur terbagi atas bagian kuning dan putih yang mempunyai nilai protein yang berbeda. Tujuan penelitian ini adalah untuk mengetahui perbedaan kadar protein putih dan kuning telur dari 2 jenis telur ayam. Pada penelitian ini teknik pengambilan sampel menggunakan metode <em>purposive sampling</em>, dimana pengambilan sampel sesuai dengan persyaratan, yaitu 2 jenis telur ayam, telur ayam ras dan telur ayam kampung dengan karakteristik tidak busuk, kulit telur tanpa bercak, kulit telur berwarna coklat/putih bersih, mutu kesegaran telur penyimpanan kurang dari 1 minggu. Metode penelitian yang dilakukan yaitu analisis kualitatif protein menggunakan metode pereaksi warna biuret, sedangkan analisis kuantitatif menggunakan spektrofotometri sinar tampak. Hasil penelitian menunjukkan bahwa kadar protein pada putih telur ayam ras memiliki kadar terendah 863,3 mg/mL dan pada kuning telur ayam kampung memiliki kadar protein tertinggi 1.229,5 mg/mL, dan kadar protein putih telur ayam kampung 945,07 mg/mL, kuning telur ayam ras 930,9 mg/mL.</p><p align="center"><strong>Kata Kunci : </strong>Protein, Telur AyamRas, Telur Ayam Kampung, Biuret, SpektrofotometriVis</p><p> </p><p><strong><em>Comparison of protein </em></strong><strong><em>level </em></strong><strong><em>in eggs </em></strong><strong><em>using </em></strong><strong><em>spectrophotometry</em></strong></p><p> </p><p><strong><em>Abstract</em></strong></p><p align="center"><strong> </strong></p><pre><em>Proteins contained in animal foods such as eggs are said to be perfect proteins. Eggs are divided into yellow and white parts that have different protein values. The purpose of this study was to determine the differences in levels of white and egg yolk protein from 2 types of chicken eggs. In this study the sampling technique used a purposive sampling method, where sampling was in accordance with the requirements, namely 2 types of chicken eggs, chicken eggs and native chicken eggs with characteristics not rot, eggshells without blotches, brown / white egg shells, quality egg storage freshness less than 1 week. The research method used was qualitative analysis of proteins using the biuret color reagent method, while quantitative analysis using visible spectrophotometry. The results showed that protein content in race chicken egg whites had the lowest levels of 863.3 mg/mL and in chicken egg yolks had the highest protein content of 1229.5 mg/mL, and the protein content of free-range egg white chicken 945.07 mg/mL, race chicken egg yolk 930.9 mg/mL</em></pre><pre><strong><em>Keywords: </em></strong><em>Protein, Ras-Chicken Egg, Village Chicken Egg, Biuret, Vis Spectrophotometry</em></pre>

Isolasi Senyawa Flavonoid Ekstrak Biji Pinang Merah (Areca vestiaria Giseke) Dan Uji Sitotoksiknya Melalui Uji Brine Shirimp Lethality Test (BSLT)

Cytotoxic test is one method to predict the development of compounds that are toxic to the cell that is an absolute requirement for anticancer drugs. The aim of this study is to isolate flavonoids and determine LC50 value of the activity of the Red Pinang seed extract (Areca vestiaria Giseke). Extracts prepared by maceration method using ethanol 96%. Isolation is done through a range of methods includes partitioning using n-hexane, a preliminary test using the color reagent and KLT, isolation by preparative thin layer chromatography, thin layer chromatography multi eluent, UV-Vis spectrophotometer analysis and cytotoxic test isolates. The results showed that isolates flavonoid seed extract of Red Pinang (Areca vestiaria Giseke) has the medium potential of being a cytotoxic agent with LC50 values of 583.445 ppm.

IDENTIFIKASI SENYAWA FLAVONOID PADA FRAKSI ETIL ASETAT BENALU BATU (Begonia sp.) ASAL KABUPATEN MOROWALI UTARA

Benalu Batu Plant (Begonia sp.) in family Begoniaceae is a plant used by the people of North Morowali to treat tumors and cancers. It generally contains saponins, tannins, flavonoids and polyphenols. This research aimed to identify flavonoid compounds contained in ethyl acetate fraction of Begonia sp. The simplicia was extracted using maceration method and partitioned using n-hexane, ethyl acetate and water. The Ethyl acetate extract was then fractionated by nine eluent combinations using separation method of Vacuum Liquid Chromatography (VLC) and Preparative Thin Layer Chromatography (Prep TLC). One fraction was obtained and was suspected to contain flavonoid compounds after Shinode and Pew color reagent test. In the result of Ultraviolet-Visible Spectroscopy with methanol at wavelength range of 200-550 nm, the isolates showed absorption peaks at 275 nm (peak 1) and 225 nm (peak 2). Based on the wavelength of flavonoid compounds contained in the fraction, Begonia sp. showed similarities to the absorption peak of flavan-3-ol or flavanols