Lipid-Controlled Stabilization of Charge-Separated States (P+QB–) and Photocurrent Generation Activity of a Light-Harvesting–Reaction Center Core Complex (LH1-RC) from Rhodopseudomonas palustris

2018 ◽  
Vol 122 (3) ◽  
pp. 1066-1080 ◽  
Author(s):  
Tomoyasu Noji ◽  
Mikano Matsuo ◽  
Nobutaka Takeda ◽  
Ayumi Sumino ◽  
Masaharu Kondo ◽  
...  
Keyword(s):  
Reaction Center ◽  
Light Harvesting ◽  
Rhodopseudomonas Palustris ◽  
Core Complex ◽  
Photocurrent Generation ◽  
Generation Activity

scholarly journals Identification of protein W, the elusive sixth subunit of the Rhodopseudomonas palustris reaction center-light harvesting 1 core complex

2018 ◽  
Vol 1859 (2) ◽  
pp. 119-128 ◽  
Author(s):  
Philip J. Jackson ◽  
Andrew Hitchcock ◽  
David J.K. Swainsbury ◽  
Pu Qian ◽  
Elizabeth C. Martin ◽  
...  
Keyword(s):  
Reaction Center ◽  
Light Harvesting ◽  
Rhodopseudomonas Palustris ◽  
Core Complex ◽  
Center Light

scholarly journals Heterologous Production of the Photosynthetic Reaction Center and Light Harvesting 1 Complexes of the Thermophile Thermochromatium tepidum in the Mesophile Rhodobacter sphaeroides and Thermal Stability of a Hybrid Core Complex

2017 ◽  
Vol 83 (20) ◽  
Author(s):  
D. Jun ◽  
V. Huang ◽  
J. T. Beatty
Keyword(s):  
Solar Cell ◽  
Reaction Center ◽  
Rhodobacter Sphaeroides ◽  
Elevated Temperatures ◽  
Light Harvesting ◽  
Partial Purification ◽  
Core Complex ◽  
Content Type ◽  
Hybrid Complex ◽  
Initial Starting

ABSTRACT The photosynthetic complexes of the thermophile Thermochromatium tepidum are of considerable interest in biohybrid solar cell applications because of the ability of thermophilic proteins to tolerate elevated temperatures. Synthetic operons encoding reaction center (RC) and light harvesting 1 (LH1) pigment-protein complexes of T. tepidum were expressed in the mesophile Rhodobacter sphaeroides. The T. tepidum RC (TRC) was assembled and was found to be functional with the addition of menadione to populate the QA pocket. The production of T. tepidum LH1 (TLH1) was increased by selection of a phototrophy-capable mutant after UV irradiation mutagenesis, which yielded a hybrid RC-TLH1 core complex consisting of the R. sphaeroides RC and T. tepidum TLH1, confirmed by the absorbance peak of TLH1 at 915 nm. Affinity chromatography partial purification and subsequent sucrose gradient analysis of the hybrid RC-TLH1 core complex indicated that this core complex assembled as a monomer. Furthermore, the RC-TLH1 hybrid core complex was more tolerant of a temperature of 70°C than the R. sphaeroides RC-LH1 core complexes in both the dimeric and monomeric forms; after 1 h, the hybrid complex retained 58% of the initial starting value, compared to values of 11% and 53% for the R. sphaeroides RC-LH1 dimer and monomer forms, respectively. IMPORTANCE This work is important because it is a new approach to bioengineering of photosynthesis proteins for potential use in biophotovoltaic solar energy capture. The work establishes a proof of principle for future biohybrid solar cell applications.

Biochemical and Spectroscopic Characterizations of a Hybrid Light-Harvesting Reaction Center Core Complex

Biochemistry ◽  
2018 ◽  
Vol 57 (30) ◽  
pp. 4496-4503 ◽  
Author(s):  
Yukihiro Kimura ◽  
Kanako Hashimoto ◽  
Seiji Akimoto ◽  
Mizuki Takenouchi ◽  
Kengo Suzuki ◽  
...  
Keyword(s):  
Reaction Center ◽  
Light Harvesting ◽  
Core Complex
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