Micrococcus luteus endonucleases for apurinic/apyrimidinic sites in deoxyribonucleic acid. 2. Further studies on the substrate specificity and mechanism of action

Biochemistry ◽  
1980 ◽  
Vol 19 (22) ◽  
pp. 5024-5029 ◽  
Author(s):  
Josiane Pierre ◽  
Jacques Laval
Keyword(s):  
Substrate Specificity ◽  
Mechanism Of Action ◽  
Deoxyribonucleic Acid ◽  
Micrococcus Luteus

Permeabilization of ultraviolet-irradiated Chinese hamster cells with polyethylene glycol and introduction of ultraviolet endonuclease from Micrococcus luteus

1981 ◽  
Vol 1 (3) ◽  
pp. 237-244
Author(s):  
D B Yarosh ◽  
R B Setlow
Keyword(s):  
Polyethylene Glycol ◽  
Deoxyribonucleic Acid ◽  
Excision Repair ◽  
Micrococcus Luteus ◽  
Chinese Hamster ◽  
Endonuclease Activity ◽  
Chinese Hamster Cells ◽  
Ultraviolet Resistance

Chinese hamster V-79 cells were made permeable by treatment with polyethylene glycol and then incubated with a Micrococcus luteus extract containing ultraviolet-specific endonuclease activity. This treatment introduced nicks in irradiated, but not in unirradiated, deoxyribonucleic acid. The nicks remained open for at least 3 h; there was no loss of endonuclease-sensitive sites, and no excision of dimers as measured by chromatography was detected. In addition, there was no increase in ultraviolet resistance in treated cells. This suggests that the absence of a significant amount of excision repair in rodent cells is due to the lack of both incision and excision capacity.

Mechanism of action of 2-haloethylnitrosoureas on deoxyribonucleic acid

1979 ◽  
Vol 28 (14) ◽  
pp. 2115-2121 ◽  
Author(s):  
J.William Lown ◽  
Larry W. McLaughlin ◽  
James A. Plambeck
Keyword(s):  
Mechanism Of Action ◽  
Deoxyribonucleic Acid

scholarly journals Permeabilization of ultraviolet-irradiated Chinese hamster cells with polyethylene glycol and introduction of ultraviolet endonuclease from Micrococcus luteus.

1981 ◽  
Vol 1 (3) ◽  
pp. 237-244 ◽  
Author(s):  
D B Yarosh ◽  
R B Setlow
Keyword(s):  
Polyethylene Glycol ◽  
Deoxyribonucleic Acid ◽  
Excision Repair ◽  
Micrococcus Luteus ◽  
Chinese Hamster ◽  
Endonuclease Activity ◽  
Chinese Hamster Cells ◽  
Ultraviolet Resistance

Chinese hamster V-79 cells were made permeable by treatment with polyethylene glycol and then incubated with a Micrococcus luteus extract containing ultraviolet-specific endonuclease activity. This treatment introduced nicks in irradiated, but not in unirradiated, deoxyribonucleic acid. The nicks remained open for at least 3 h; there was no loss of endonuclease-sensitive sites, and no excision of dimers as measured by chromatography was detected. In addition, there was no increase in ultraviolet resistance in treated cells. This suggests that the absence of a significant amount of excision repair in rodent cells is due to the lack of both incision and excision capacity.

scholarly journals Aldehyde Dehydrogenase Inhibitors: a Comprehensive Review of the Pharmacology, Mechanism of Action, Substrate Specificity, and Clinical Application

2012 ◽  
Vol 64 (3) ◽  
pp. 520-539 ◽  
Author(s):  
Vindhya Koppaka ◽  
David C. Thompson ◽  
Ying Chen ◽  
Manuel Ellermann ◽  
Kyriacos C. Nicolaou ◽  
...  
Keyword(s):  
Substrate Specificity ◽  
Clinical Application ◽  
Aldehyde Dehydrogenase ◽  
Mechanism Of Action ◽  
Comprehensive Review

Brain histone kinase: The structure, the substrate specificity and the mechanism of action

1976 ◽  
Vol 14 ◽  
pp. 407-444 ◽  
Author(s):  
E.S. Severin ◽  
M.V. Nesterova ◽  
N.N. Gulyaev ◽  
S.V. Shlyapnikov
Keyword(s):  
Substrate Specificity ◽  
Mechanism Of Action ◽  
Histone Kinase
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