Determination of the equilibrium distribution between alcohol and aldehyde substrates when bound to horse liver alcohol dehydrogenase using magnetic resonance

Biochemistry ◽  
1980 ◽  
Vol 19 (24) ◽  
pp. 5486-5493 ◽  
Author(s):  
D. C. Anderson ◽  
F. W. Dahlquist
Keyword(s):  
Magnetic Resonance ◽  
Alcohol Dehydrogenase ◽  
Equilibrium Distribution ◽  
Horse Liver Alcohol Dehydrogenase ◽  
Liver Alcohol Dehydrogenase ◽  
Horse Liver

scholarly journals On the Determination of the N-Terminal Groups of Horse Liver Alcohol Dehydrogenase.

1965 ◽  
Vol 19 ◽  
pp. 1483-1485 ◽  
Author(s):  
Hans Jörnvall ◽  
K. Hartiala ◽  
Terje Skansen ◽  
J. Brunvoll ◽  
Merv Hinton
Keyword(s):  
Alcohol Dehydrogenase ◽  
Horse Liver Alcohol Dehydrogenase ◽  
Liver Alcohol Dehydrogenase ◽  
Horse Liver

Analysis of one enzyme recyclization system with low enzyme concentration

1982 ◽  
Vol 47 (12) ◽  
pp. 3339-3347
Author(s):  
Jan Kovář ◽  
Igor Kučera
Keyword(s):  
Alcohol Dehydrogenase ◽  
Enzyme Concentration ◽  
Horse Liver Alcohol Dehydrogenase ◽  
Liver Alcohol Dehydrogenase ◽  
Horse Liver ◽  
Kinetics Of ◽  
Analytical Practice

One enzyme recyclization reactions in the presence of two substrates and a small amount of a coenzyme are characterized by a long prestationary phase, in the course of which the distribution of coenzyme forms varies. The kinetics of the coenzyme prestationary phase was analyzed theoretically and some equations characterizing mainly the start and the end of this phase were derived. To verify experimentally the derived equations two recyclization systems with horse liver alcohol dehydrogenase, NAD or NADH, and corresponding substrate pairs (p-nitrosodimethylaniline and 1-butanol, or furyl alcohol and acetaldehyde) were investigated. Consequences for the analytical practice and utilization of these reactions for the determination of small coenzyme and enzyme concentrations are discussed.

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