Fnr, a Global Transcriptional Regulator of Escherichia coli, Activates the Vitreoscilla Hemoglobin (VHb) Promoter and Intracellular VHb Expression Increases Cytochrome d Promoter Activity

1995 ◽  
Vol 11 (3) ◽  
pp. 288-293 ◽  
Author(s):  
Philip S. Tsai ◽  
Pauli T. Kallio ◽  
James E. Bailey
Keyword(s):  
Escherichia Coli ◽  
Promoter Activity ◽  
Transcriptional Regulator ◽  
Vitreoscilla Hemoglobin ◽  
Cytochrome D

scholarly journals Identification of subunit I as the cytochrome b558 component of the cytochrome d terminal oxidase complex of Escherichia coli.

1984 ◽  
Vol 259 (12) ◽  
pp. 7994-7997 ◽  
Author(s):  
G N Green ◽  
R G Kranz ◽  
R M Lorence ◽  
R B Gennis
Keyword(s):  
Escherichia Coli ◽  
Terminal Oxidase ◽  
Cytochrome B558 ◽  
Cytochrome D

scholarly journals Protein composition of Vitreoscilla hemoglobin inclusion bodies produced in Escherichia coli.

1990 ◽  
Vol 265 (21) ◽  
pp. 12728-12733
Author(s):  
R A Hart ◽  
U Rinas ◽  
J E Bailey
Keyword(s):  
Escherichia Coli ◽  
Inclusion Bodies ◽  
Protein Composition ◽  
Vitreoscilla Hemoglobin

scholarly journals Molecular characterization of the Escherichia coli htrD gene: cloning, sequence, regulation, and involvement with cytochrome d oxidase.

1993 ◽  
Vol 175 (1) ◽  
pp. 166-175 ◽  
Author(s):  
J M Delaney ◽  
D Wall ◽  
C Georgopoulos
Keyword(s):  
Escherichia Coli ◽  
Gene Cloning ◽  
Molecular Characterization ◽  
Cytochrome D

Maximum rrn promoter activity in Escherichia coli at saturating concentrations of free RNA polymerase

Biochimie ◽  
2010 ◽  
Vol 92 (1) ◽  
pp. 12-20 ◽  
Author(s):  
M. Ehrenberg ◽  
P.P. Dennis ◽  
H. Bremer
Keyword(s):  
Escherichia Coli ◽  
Rna Polymerase ◽  
Promoter Activity

Expression of Double Vitreoscilla Hemoglobin Enhances Growth and Alters Ribosome and tRNA Levels in Escherichia coli

2002 ◽  
Vol 18 (3) ◽  
pp. 652-656 ◽  
Author(s):  
V. Roos ◽  
C.I.J. Andersson ◽  
C. Arfvidsson ◽  
K.-G. Wahlund ◽  
L. Bulow
Keyword(s):  
Escherichia Coli ◽  
Vitreoscilla Hemoglobin

Iron influences the expression of colonization factor CS6 of enterotoxigenic Escherichia coli

Microbiology ◽  
2021 ◽  
Vol 167 (9) ◽  
Author(s):  
Debjyoti Bhakat ◽  
Indranil Mondal ◽  
Asish Kumar Mukhopadhyay ◽  
Nabendu Sekhar Chatterjee
Keyword(s):  
Escherichia Coli ◽  
Promoter Activity ◽  
Iron Concentration ◽  
Iron Chelation ◽  
Enterotoxigenic Escherichia Coli ◽  
Watery Diarrhoea ◽  
Rna Expression ◽  
Iron Availability ◽  
Iron Salt ◽  
Ht 29

Enterotoxigenic Escherichia coli (ETEC) is a major pathogen of acute watery diarrhoea. The pathogenicity of ETEC is linked to adherence to the small intestine by colonization factors (CFs) and secretion of heat-labile enterotoxin (LT) and/or heat-stable enterotoxin (ST). CS6 is one of the most common CFs in our region and worldwide. Iron availability functions as an environmental cue for enteropathogenic bacteria, signalling arrival within the human host. Therefore, iron could modify the expression of CS6 in the intestine. The objective of this study was to determine the effect of iron availability on CS6 expression in ETEC. This would help in understanding the importance of iron during ETEC pathogenesis. ETEC strain harbouring CS6 was cultured under increasing concentrations of iron salt to assess the effect on CS6 RNA expression by quantitative RT-PCR, protein expression by ELISA, promoter activity by β-galactosidase activity, and epithelial adhesion on HT-29 cells. RNA expression of CS6 was maximum in presence of 0.2 mM iron (II) salt. The expression increased by 50-fold, which also reduced under iron-chelation conditions and an increased iron concentration of 0.4 mM or more. The surface expression of CS6 also increased by 60-fold in presence of 0.2 mM iron. The upregulation of CS6 promoter activity by 25-fold under this experimental condition was in accordance with the induction of CS6 RNA and protein. This increased CS6 expression was independent of ETEC strains. Bacterial adhesion to HT-29 epithelial cells was also enhanced by five-fold in the presence of 0.2 mM iron salt. These findings suggest that CS6 expression is dependent on iron concentration. However, with further increases in iron concentration beyond 0.2 mM CS6 expression is decreased, suggesting that there might be a strong regulatory mechanism for CS6 expression under different iron concentrations.

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