Transcript secondary structures regulate transcription termination at the attenuator of S. marcescens tryptophan operon

Nature ◽  
1982 ◽  
Vol 298 (5869) ◽  
pp. 34-38 ◽  
Author(s):  
I. Stroynowski ◽  
C. Yanofsky
Keyword(s):  
Transcription Termination ◽  
Secondary Structures ◽  
Tryptophan Operon

Regulatory region of the Klebsiella aerogenes tryptophan operon

1982 ◽  
Vol 152 (1) ◽  
pp. 49-56
Author(s):  
M Blumenberg ◽  
C Yanofsky
Keyword(s):  
Transcription Termination ◽  
Regulatory Region ◽  
Enteric Bacteria ◽  
In Vitro Transcription ◽  
Regulatory Sequences ◽  
Klebsiella Aerogenes ◽  
Transcription Termination Site ◽  
Tryptophan Operon ◽  
Trp Promoter

The trp operon of Klebsiella aerogenes was cloned, and its regulatory region was sequenced. Comparison with previously reported trp regulatory sequences of other enteric bacteria indicates that the K. aerogenes trp promoter-operator region is most similar to the corresponding region of Salmonella typhimurium. The trp leader regions of K. aerogenes and other enteric bacteria are organized similarly, but there are significant differences in the stabilities of the predicted secondary structures in their leader transcripts. These differences should make the K. aerogenes attenuator a weaker transcription termination site than any of the other attenuator regions studied; this was confirmed in in vitro transcription experiments. The sequence of the leader transcript and the precise site of in vitro termination were determined.

Transcription termination at the end of the tryptophan operon of Escherichia coli

1977 ◽  
Vol 112 (3) ◽  
pp. 423-436 ◽  
Author(s):  
Leonard P. Guarente ◽  
David H. Mitchell ◽  
Jon Beckwith
Keyword(s):  
Escherichia Coli ◽  
Transcription Termination ◽  
Tryptophan Operon
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