scholarly journals MicroRNA-195 targets ADP-ribosylation factor-like protein 2 to induce apoptosis in human embryonic stem cell-derived neural progenitor cells

2013 ◽  
Vol 4 (6) ◽  
pp. e695-e695 ◽  
Author(s):  
Y Zhou ◽  
H Jiang ◽  
J Gu ◽  
Y Tang ◽  
N Shen ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Progenitor Cells ◽  
Human Embryonic Stem Cell ◽  
Neural Progenitor Cells ◽  
Embryonic Stem ◽  
Neural Progenitor ◽  
Adp Ribosylation ◽  
Adp Ribosylation Factor

scholarly journals Potential for cell therapy in Parkinson's disease using genetically programmed human embryonic stem cell-derived neural progenitor cells

2014 ◽  
Vol 522 (12) ◽  
pp. 2845-2856 ◽  
Author(s):  
Rajesh Ambasudhan ◽  
Nima Dolatabadi ◽  
Anthony Nutter ◽  
Eliezer Masliah ◽  
Scott R. Mckercher ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Stem Cell ◽  
Cell Therapy ◽  
Embryonic Stem Cell ◽  
Progenitor Cells ◽  
Human Embryonic Stem Cell ◽  
Neural Progenitor Cells ◽  
Embryonic Stem ◽  
Neural Progenitor

The ROCK Inhibitor Y-26732 Enhances the Survival and Proliferation of Human Embryonic Stem Cell-Derived Neural Progenitor Cells upon Dissociation

2013 ◽  
Vol 198 (2) ◽  
pp. 127-138 ◽  
Author(s):  
Ruttachuk Rungsiwiwut ◽  
Chirawattana Manolertthewan ◽  
Pranee Numchaisrika ◽  
Vichuda Ahnonkitpanit ◽  
Pramuan Virutamasen ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Progenitor Cells ◽  
Human Embryonic Stem Cell ◽  
Neural Progenitor Cells ◽  
Embryonic Stem ◽  
Neural Progenitor ◽  
Rock Inhibitor

scholarly journals Engraftment and differentiation of embryonic stem cell–derived neural progenitor cells in the cochlear nerve trunk: Growth of processes into the organ of corti

2006 ◽  
Vol 66 (13) ◽  
pp. 1489-1500 ◽  
Author(s):  
C. Eduardo Corrales ◽  
Luying Pan ◽  
Huawei Li ◽  
M. Charles Liberman ◽  
Stefan Heller ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Progenitor Cells ◽  
Neural Progenitor Cells ◽  
Embryonic Stem ◽  
Organ Of Corti ◽  
Neural Progenitor ◽  
Nerve Trunk ◽  
Cochlear Nerve

Electromagnetic fields affect transcript levels of apoptosis‐related genes in embryonic stem cell‐derived neural progenitor cells

2005 ◽  
Vol 19 (12) ◽  
pp. 1686-1688 ◽  
Author(s):  
Teodora Nikolova ◽  
Jaroslaw Czyz ◽  
Alexandra Rolletschek ◽  
Przemyslaw Blyszczuk ◽  
Jörg Fuchs ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Progenitor Cells ◽  
Electromagnetic Fields ◽  
Neural Progenitor Cells ◽  
Embryonic Stem ◽  
Neural Progenitor ◽  
Transcript Levels

REPROGRAMMING OF PORCINE EPIBLAST-DERIVED NEURAL PROGENITOR CELLS TO PLURIPOTENCY

2012 ◽  
Vol 24 (1) ◽  
pp. 289
Author(s):  
M. A. Rasmussen ◽  
V. J. Hall ◽  
S. G. Petkov ◽  
O. Ujhelly ◽  
M. Pirity ◽  
...  
Keyword(s):  
Stem Cell ◽  
Progenitor Cells ◽  
Neural Progenitor Cells ◽  
Autologous Transplantation ◽  
Embryonic Stem ◽  
Neural Progenitor ◽  
Embryoid Bodies ◽  
Advanced Technology ◽  
Feeder Cells ◽  
Pluripotency Genes

Human induced pluripotent stem cells (iPSC) and neural progenitor cells (NPC) are envisioned to play a vital role in future cell replacement therapy. In this context, porcine iPSC and NPC would be highly useful for pre-clinical safety testing by autologous transplantation in a porcine biomedical model. The objective of this study was to establish iPSC from porcine epiblast-derived NPC by use of a tetracycline-inducible Tet-ON approach. A total of 1.5 × 105 porcine NPC at passage 6 (Rasmussen et al. 2011) were transduced O/N with 0.5 ml active virus containing the following porcine pluripotency genes: pOCT4 (pO); pOCT4 and pKLF4 (pOK); pOCT4 and pC-MYC (pOM); pOCT4, pC-MYC, and pKLF4 (pOMK) or polycistronic pOCT4, pSOX2, pC-MYC, and pKLF4 (pOSMK); all including 0.25 ml transactivator (rtTA). After 3 days, the cells were trypsinized and passaged to MEF feeder cells and cultured in iPSC medium containing DMEM/F12, 20% KSR, 1% NEAA, 10 μM β-Me, 20 ng mL–1 human bFGF and 2 μg mL–1 doxycycline. On Day 8, tightly packed colonies of cells presenting an embryonic stem cell-like morphology were visible in the pOM, pOMK, and pOSMK combinations. In contrast, colonies were not observed with the pO and pOK combination. On Day 14, several iPSC-like colonies were manually picked and sub-cultured on MEF feeder cells in iPSC medium. Two lines from the pOSMK combination were capable of prolonged clonal propagation while maintaining an ESC-like morphology. However, when doxycycline was removed from the culture medium, growth arrest and spontaneous differentiation occurred. The iPSC-like lines expressed OCT4, SOX2, C-MYC, and KLF4, as evaluated by immunocytochemistry, and expression of NANOG, SSEA-1, and SSEA-4 was also confirmed, demonstrating activation of endogenous pluripotency genes. The iPSC-like lines were capable of forming embryoid bodies (EB) without addition of doxycycline and in vitro differentiation of EB in medium containing DMEM and 15% FCS confirmed the presence of meso- (SMA) and endodermal (AFP) derivatives by immunocytochemistry. Furthermore, co-culture experiments with MS5 stromal cells in medium containing DMEM, 15% KSR, and 150 ng mL–1 human Noggin resulted in differentiation into neuroectoderm (NESTIN and SOX2), as well as more mature neurons (TUJI and GFAP). The latter resulted in establishment of new NPC lines. The system can be used to study mechanisms involved in the early transition from pluripotency to multipotency in the pig and the reversal of the process caused by reprogramming. The Danish Agency for Science, Technology and Innovation, the Danish National Advanced Technology Foundation as well as the EU projects, EU FP7 Stem Cell Project “PartnErS” (218205; 204, 523) and EU FP7 Stem Cell Project “PluriSys” (223485).

scholarly journals Enrichment of human embryonic stem cell-derived NKX6.1-expressing pancreatic progenitor cells accelerates the maturation of insulin-secreting cells in vivo

Stem Cells ◽  
2013 ◽  
Vol 31 (11) ◽  
pp. 2432-2442 ◽  
Author(s):  
Alireza Rezania ◽  
Jennifer E. Bruin ◽  
Jean Xu ◽  
Kavitha Narayan ◽  
Jessica K. Fox ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Progenitor Cells ◽  
Human Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Pancreatic Progenitor ◽  
Pancreatic Progenitor Cells ◽  
Insulin Secreting Cells
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