Use of a phosphorylated spin trap to discriminate between the hydroxyl radical and other oxidising species

1998 ◽  
pp. 1715-1718 ◽  
Author(s):  
Jean-Louis Clément ◽  
Bruce C. Gilbert ◽  
Win F. Ho ◽  
Natalie D. Jackson ◽  
Maurice S. Newton ◽  
...  
Keyword(s):  
Hydroxyl Radical ◽  
Spin Trap

scholarly journals The spin trapping of pyrimidine nucleotide free radicals in a Fenton system

1989 ◽  
Vol 261 (3) ◽  
pp. 831-839 ◽  
Author(s):  
W D Flitter ◽  
R P Mason
Keyword(s):  
Free Radicals ◽  
Hydroxyl Radical ◽  
Spin Trap ◽  
Gamma Ray ◽  
Thiobarbituric Acid ◽  
Spin Trapping ◽  
Pyrimidine Nucleotide ◽  
Gamma Ray Irradiation ◽  
Radical Attack ◽  
Fenton System

The reaction of the hydroxyl radical, generated by a Fenton system, with pyrimidine deoxyribonucleotides was investigated by using the e.s.r. technique of spin trapping. The spin trap t-nitrosobutane was employed to trap secondary radicals formed by the reaction of the hydroxyl radical with these nucleotides. The results presented here show that hydroxyl-radical attack on thymidine, 2-deoxycytidine 5-monophosphate and 2-deoxyuridine 5-monophosphate produced nucleotide-derived free radicals. The results indicate that .OH radical attack occurs predominantly at the carbon-carbon double bond of the pyrimidine base. The e.s.r. studies showed a good correlation with previous results obtained by authors who used x- or gamma-ray irradiation to generate the hydroxyl radical. A thiobarbituric acid assay was also used to monitor the damage produced to the nucleotides by the Fenton system. These results showed qualitative agreement with the spin-trapping studies.

Photolysis of chlorpromazine: hydroxyl radical detection using 2-methyl-2-nitrosopropane as a spin trap

1982 ◽  
Vol 20 (2) ◽  
pp. 169-174 ◽  
Author(s):  
Y. Lion ◽  
J. Decuyper ◽  
A. van de Vorst ◽  
J. Piette
Keyword(s):  
Hydroxyl Radical ◽  
Spin Trap ◽  
Radical Detection

ChemInform Abstract: Use of a Phosphorylated Spin Trap to Discriminate Between the Hydroxyl Radical and Other Oxidizing Species.

ChemInform ◽  
2010 ◽  
Vol 29 (49) ◽  
pp. no-no
Author(s):  
J.-L. CLEMENT ◽  
B. C. GILBERT ◽  
W. F. HO ◽  
N. D. JACKSON ◽  
M. S. NEWTON ◽  
...  
Keyword(s):  
Hydroxyl Radical ◽  
Spin Trap

A One-Step Synthesis of 2-(2-Pyridyl)-3H-indol-3-oneN-Oxide:  Is It an Efficient Spin Trap for Hydroxyl Radical?

2000 ◽  
Vol 65 (14) ◽  
pp. 4460-4463 ◽  
Author(s):  
Gerald M. Rosen ◽  
Pei Tsai ◽  
Eugene D. Barth ◽  
Gilbert Dorey ◽  
Patrick Casara ◽  
...  
Keyword(s):  
Hydroxyl Radical ◽  
Spin Trap ◽  
One Step

Hydroxyl Radical Generation Caused by the Reaction of Singlet Oxygen with a Spin Trap, DMPO, Increases Significantly in the Presence of Biological Reductants

2004 ◽  
Vol 38 (4) ◽  
pp. 385-392 ◽  
Author(s):  
Chiho Nishizawa ◽  
Keizo Takeshita ◽  
Jun-ichi Ueda ◽  
Michiko Mizuno ◽  
Kazuo T. Suzuki ◽  
...  
Keyword(s):  
Hydroxyl Radical ◽  
Singlet Oxygen ◽  
Spin Trap ◽  
Radical Generation

Melatonin prevents the increase in hydroxyl radical-spin trap adduct formation caused by the addition of cisplatin in vitro

Life Sciences ◽  
2003 ◽  
Vol 72 (15) ◽  
pp. 1773-1780 ◽  
Author(s):  
Minoru Yoshida ◽  
Atsushi Fukuda ◽  
Masayuki Hara ◽  
Atsushi Terada ◽  
Yousuke Kitanaka ◽  
...  
Keyword(s):  
Hydroxyl Radical ◽  
Spin Trap ◽  
Adduct Formation

5-Carboxy-5-methyl-1-pyrroline N-oxide: a spin trap for the hydroxyl radical

2001 ◽  
pp. 875-880 ◽  
Author(s):  
Pei Tsai ◽  
Martyna Elas† ◽  
Adrian D. Parasca ◽  
Eugene D. Barth ◽  
Colin Mailer ◽  
...  
Keyword(s):  
Hydroxyl Radical ◽  
Spin Trap
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