Engineering a large protein by combined rational and random approaches: stabilizing the Clostridium thermocellum cellobiose phosphorylase

2012 ◽  
Vol 8 (6) ◽  
pp. 1815 ◽  
Author(s):  
Xinhao Ye ◽  
Chenming Zhang ◽  
Y.-H. Percival Zhang
Keyword(s):  
Clostridium Thermocellum ◽  
Large Protein ◽  
Cellobiose Phosphorylase

Efficient chemoenzymatic oligosaccharide synthesis by reverse phosphorolysis using cellobiose phosphorylase and cellodextrin phosphorylase from Clostridium thermocellum

Biochimie ◽  
2010 ◽  
Vol 92 (12) ◽  
pp. 1818-1826 ◽  
Author(s):  
Hiroyuki Nakai ◽  
Maher Abou Hachem ◽  
Bent O. Petersen ◽  
Yvonne Westphal ◽  
Karin Mannerstedt ◽  
...  
Keyword(s):  
Clostridium Thermocellum ◽  
Oligosaccharide Synthesis ◽  
Cellobiose Phosphorylase

Purification and properties of cellobiose phosphorylase from Clostridium thermocellum

1995 ◽  
Vol 79 (3) ◽  
pp. 212-216 ◽  
Author(s):  
Keijitsu Tanaka ◽  
Takashi Kawaguchi ◽  
Yukio Imada ◽  
Toshihiko Ooi ◽  
Motoo Arai
Keyword(s):  
Clostridium Thermocellum ◽  
Purification And Properties ◽  
Cellobiose Phosphorylase

Fusion and Fission Processes in Exocytosis: Possible Roles for Synexin and Osmotic Lysis in the Two Events

1980 ◽  
Vol 38 ◽  
pp. 594-597
Author(s):  
H.B. Pollard ◽  
C.E. Creutz ◽  
C.J. Pazoles ◽  
J.H. Scott
Keyword(s):  
Small Molecules ◽  
Chromaffin Cells ◽  
Adrenal Glands ◽  
General Concept ◽  
Extracellular Calcium ◽  
Large Protein ◽  
Granule Membrane ◽  
Osmotic Lysis ◽  
Per Se ◽  
Chemical Evidence

Exocytosis is a general concept describing secretion of enzymes, hormones and transmitters that are otherwise sequestered in intracellular granules. Chemical evidence for this concept was first gathered from studies on chromaffin cells in perfused adrenal glands, in which it was found that granule contents, including both large protein and small molecules such as adrenaline and ATP, were released together while the granule membrane was retained in the cell. A number of exhaustive reviews of this early work have been published and are summarized in Reference 1. The critical experiments demonstrating the importance of extracellular calcium for exocytosis per se were also first performed in this system (2,3), further indicating the substantial service given by chromaffin cells to those interested in secretory phenomena over the years.

Exemplar Abstract for Clostridium thermocellum Viljoen et al. 1926 (Approved Lists 1980).

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Dorothea Taylor ◽  
George M Garrity
Keyword(s):  
Clostridium Thermocellum
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