A single-molecule force-spectroscopic study on stabilization of G-quadruplex DNA by a telomerase inhibitor

The Analyst ◽  
2014 ◽  
Vol 139 (16) ◽  
pp. 4037-4043 ◽  
Author(s):  
Ryoto Funayama ◽  
Yoshio Nakahara ◽  
Shinpei Kado ◽  
Mutsuo Tanaka ◽  
Keiichi Kimura
Keyword(s):  
Spectroscopic Study ◽  
Single Molecule ◽  
Quadruplex Dna ◽  
Telomerase Inhibitor ◽  
G Quadruplex

The stabilization of G-quadruplex DNA by a telomerase inhibitor was semi-quantitatively evaluated by AFM-based SMFS.

scholarly journals Characterizing the Binding of G-Quadruplex DNA to Insulin by Single Molecule and Bulk Ensemble Methods

2012 ◽  
Vol 102 (3) ◽  
pp. 75a
Author(s):  
Christine Timmer ◽  
Amanda Witte ◽  
Niecia Flikweert ◽  
Kumar Sinniah
Keyword(s):  
Single Molecule ◽  
Ensemble Methods ◽  
Quadruplex Dna ◽  
G Quadruplex

scholarly journals The Binding of G‐Quadruplex DNA to Insulin: From Single Molecule to Bulk‐Methods

2012 ◽  
Vol 26 (S1) ◽  
Author(s):  
Kumar Sinniah ◽  
Susanna Lynch ◽  
Christine Timmer ◽  
Amanda Witte
Keyword(s):  
Single Molecule ◽  
Quadruplex Dna ◽  
G Quadruplex

scholarly journals A Single Molecule Force Spectroscopy Study of the Binding Interaction Between Insulin and G-Quadruplex DNA

2010 ◽  
Vol 98 (3) ◽  
pp. 592a
Author(s):  
Susanna Lynch ◽  
Rachel Battershell ◽  
Jordan Scott ◽  
Dejian Zhou ◽  
Kumar Sinniah
Keyword(s):  
Single Molecule ◽  
Force Spectroscopy ◽  
Spectroscopy Study ◽  
Binding Interaction ◽  
Single Molecule Force Spectroscopy ◽  
Quadruplex Dna ◽  
G Quadruplex

Interactions between a Bioflavonoid and c-MYC Promoter G-Quadruplex DNA: Ensemble and Single-Molecule Investigations

2019 ◽  
Vol 123 (9) ◽  
pp. 2022-2031 ◽  
Author(s):  
Sneha Paul ◽  
Sk Saddam Hossain ◽  
Bala Divya M ◽  
Anunay Samanta
Keyword(s):  
Single Molecule ◽  
Quadruplex Dna ◽  
G Quadruplex

Spectroscopic study and G-quadruplex DNA binding affinity of two bioactive papaverine-derived ligands

2007 ◽  
Vol 41 (5) ◽  
pp. 558-563 ◽  
Author(s):  
Elzbieta Galezowska ◽  
Anna Masternak ◽  
Blazej Rubis ◽  
Artur Czyrski ◽  
Maria Rybczyńska ◽  
...  
Keyword(s):  
Dna Binding ◽  
Spectroscopic Study ◽  
Binding Affinity ◽  
Quadruplex Dna ◽  
G Quadruplex ◽  
Dna Binding Affinity

scholarly journals Single Molecule Force Spectroscopy on G-Quadruplex DNA

2009 ◽  
Vol 15 (33) ◽  
pp. 8113-8116 ◽  
Author(s):  
Susanna Lynch ◽  
Heather Baker ◽  
Sarah G. Byker ◽  
Dejian Zhou ◽  
Kumar Sinniah
Keyword(s):  
Single Molecule ◽  
Force Spectroscopy ◽  
Single Molecule Force Spectroscopy ◽  
Quadruplex Dna ◽  
G Quadruplex

Targeting G-quadruplex DNA and B-DNA with a natural alkaloid: a comparative spectroscopic study

RSC Advances ◽  
2016 ◽  
Vol 6 (111) ◽  
pp. 109846-109856 ◽  
Author(s):  
Paulami Mandal ◽  
Maitree Bhattacharya ◽  
Joydeep Chowdhury
Keyword(s):  
Molecular Recognition ◽  
Spectroscopic Study ◽  
Quadruplex Dna ◽  
G Quadruplex ◽  
Natural Alkaloid

Different modes of binding of natural alkaloid harmine with G-quadruplex DNA and B-DNA: key roles in molecular recognition.

scholarly journals Multicharged Phthalocyanines as Selective Ligands for G-Quadruplex DNA Structures

Molecules ◽  
2019 ◽  
Vol 24 (4) ◽  
pp. 733 ◽  
Author(s):  
Catarina Ramos ◽  
Susana Almeida ◽  
Leandro Lourenço ◽  
Patrícia Pereira ◽  
Rosa Fernandes ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Spectroscopic Techniques ◽  
Duplex Dna ◽  
Quadruplex Dna ◽  
Dna Structures ◽  
Telomerase Inhibitor ◽  
G Quadruplex ◽  
Bladder Cancer Cells ◽  
Selective Ligands ◽  
Inhibitor Selectivity

The stabilization of G-Quadruplex DNA structures by ligands is a promising strategy for telomerase inhibition in cancer therapy since this enzyme is responsible for the unlimited proliferation of cancer cells. To assess the potential of a compound as a telomerase inhibitor, selectivity for quadruplex over duplex DNA is a fundamental attribute, as the drug must be able to recognize quadruplex DNA in the presence of a large amount of duplex DNA, in the cellular nucleus. By using different spectroscopic techniques, such as ultraviolet-visible, fluorescence and circular dichroism, this work evaluates the potential of a series of multicharged phthalocyanines, bearing four or eight positive charges, as G-Quadruplex stabilizing ligands. This work led us to conclude that the existence of a balance between the number and position of the positive charges in the phthalocyanine structure is a fundamental attribute for its selectivity for G-Quadruplex structures over duplex DNA structures. Two of the studied phthalocyanines, one with four peripheral positive charges (ZnPc1) and the other with less exposed eight positive charges (ZnPc4) showed high selectivity and affinity for G-Quadruplex over duplex DNA structures and were able to accumulate in the nucleus of UM-UC-3 bladder cancer cells.

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