The isolated C-terminus of polycystin-1 promotes increased ATP-stimulated chloride secretion in a collecting duct cell line

2003 ◽  
Vol 104 (3) ◽  
pp. 217-221 ◽  
Author(s):  
Kimberly M. HOOPER ◽  
Robert J. UNWIN ◽  
Michael SUTTERS
Keyword(s):  
Fusion Protein ◽  
Cell Line ◽  
Collecting Duct ◽  
Basolateral Membrane ◽  
Duct Cell ◽  
Chloride Secretion ◽  
Cortical Collecting Duct ◽  
Membrane Expression ◽  
Fusion Protein Expression ◽  
C Terminus

Cyst expansion in autosomal dominant polycystic kidney disease (ADPKD) requires accumulation of fluid into the cyst lumen, which is probably driven by aberrant chloride secretion by the cyst lining epithelium. Extracellular ATP is a potent stimulus for chloride secretion in many epithelial systems, and provides a plausible mechanism for secretion in ADPKD. Therefore the link between polycystin-1 and ATP-stimulated chloride secretion was investigated in the M1 cortical collecting duct cell line. M1 cells were stably transfected with a glucocorticoid-inducible cytoplasmic C-terminal polycystin-1 construct fused to a membrane expression cassette. Induction of fusion protein expression was associated with augmentation of ATP-stimulated transepithelial chloride secretion. After nystatin-induced permeabilization of the basolateral membrane, it was determined that expression of the polycystin fusion protein modulated an ATP-responsive apical chloride conductance. It is concluded that up-regulation of ATP-stimulated chloride secretion might play a significant role in cyst expansion in ADPKD.

The isolated C-terminus of polycystin-1 promotes increased ATP-stimulated chloride secretion in a collecting duct cell line

2003 ◽  
Vol 104 (3) ◽  
pp. 217 ◽  
Author(s):  
Kimberly M. HOOPER ◽  
Robert J. UNWIN ◽  
Michael SUTTERS
Keyword(s):  
Cell Line ◽  
Collecting Duct ◽  
Duct Cell ◽  
Chloride Secretion ◽  
C Terminus

scholarly journals Basolateral Translocation by Vasopressin of the Aldosterone-Induced Pool of Latent Na-K-ATPases Is Accompanied by α1 Subunit Dephosphorylation: Study in a New Aldosterone-Sensitive Rat Cortical Collecting Duct Cell Line

2001 ◽  
Vol 12 (9) ◽  
pp. 1805-1818
Author(s):  
SABRI DJELIDI ◽  
AHMED BEGGAH ◽  
NATHALIE COURTOIS-COUTRY ◽  
MICHEL FAY ◽  
FRANCOISE CLUZEAUD ◽  
...  
Keyword(s):  
Cell Line ◽  
Mineralocorticoid Receptor ◽  
Collecting Duct ◽  
Short Circuit ◽  
Basolateral Membrane ◽  
Short Circuit Current ◽  
Duct Cell ◽  
Cortical Collecting Duct ◽  
Α Subunit ◽  
Α1 Subunit

Abstract. The regulation of plasma membrane Na+-K+-ATPases (NKA) expression by aldosterone and arginin vasopressin (AVP) in the cortical collecting duct (CCD) has been examined in a new rat CCD cell line, designated as RCCD2. This cell line has maintained many characteristics of the CCD—in particular, the expression of the mineralocorticoid receptor. Mineralocorticoid receptor is expressed at the protein level and binds3H-aldosterone (approximately 15 to 20 fmol/mg protein). Short-circuit current (Isc) experiments showed approximately a twofold increase in Isc associated with a decrease in transepithelial resistance when cells were treated with aldosterone concentrations as low as 10-9M. This effect on Isc was significant 2 h after aldosterone addition and was still present after 24 h. It was accompanied by an increase in the amount of mRNA encoding for the α subunit of the epithelial sodium channel (sixfold) and the α1 subunit of NKA (fourfold) after 24 h of hormone treatment. In addition, mRNA expression of the serum- and glucocorticoid-induced kinase (Sgk) was increased by 10-9M aldosterone treatment as early as 45 min after hormone addition. As had already been documented in native CCD obtained by microdissection, incubation of RCCD2cells for 24 h with aldosterone resulted in the constitution of a latent pool of NKA that could be rapidly recruited by AVP (15 min). NKA biotinylation experiments and preparation of membrane fractions show that this latent pool of NKA is present in the intracellular compartment of the cells and is recruited by AVP in the basolateral membrane through a translocation process. This mechanism is accompanied by dephosphorylation of the α1catalytic subunit of NKA.

scholarly journals Effects of chemical chaperones on partially retarded NaCl cotransporter mutants associated with Gitelman's syndrome in a mouse cortical collecting duct cell line

2004 ◽  
Vol 19 (5) ◽  
pp. 1069-1076 ◽  
Author(s):  
J. C. de Jong ◽  
P. H. G. M. Willems ◽  
M. Goossens ◽  
A. Vandewalle ◽  
L. P. W. J. van den Heuvel ◽  
...  
Keyword(s):  
Cell Line ◽  
Collecting Duct ◽  
Duct Cell ◽  
Cortical Collecting Duct ◽  
Chemical Chaperones ◽  
Gitelman's Syndrome ◽  
Nacl Cotransporter

scholarly journals Transcriptome of a mouse kidney cortical collecting duct cell line: Effects of aldosterone and vasopressin

2001 ◽  
Vol 98 (5) ◽  
pp. 2712-2716 ◽  
Author(s):  
M. Robert-Nicoud ◽  
M. Flahaut ◽  
J.-M. Elalouf ◽  
M. Nicod ◽  
M. Salinas ◽  
...  
Keyword(s):  
Cell Line ◽  
Collecting Duct ◽  
Duct Cell ◽  
Mouse Kidney ◽  
Cortical Collecting Duct

Vasopressin Regulates Water Flow in a Rat Cortical Collecting Duct Cell Line Not Containing Known Aquaporins

2001 ◽  
Vol 179 (1) ◽  
pp. 63-70 ◽  
Author(s):  
C. Capurro ◽  
V. Rivarola ◽  
A. Kierbel ◽  
B. Escoubet ◽  
N. Farman ◽  
...  
Keyword(s):  
Cell Line ◽  
Water Flow ◽  
Collecting Duct ◽  
Duct Cell ◽  
Cortical Collecting Duct

scholarly journals Receptors for natriuretic peptides in a human cortical collecting duct cell line

1997 ◽  
Vol 51 (1) ◽  
pp. 281-287 ◽  
Author(s):  
Valerie Millul ◽  
Nicole Ardaillou ◽  
Sandrine Placier ◽  
Béatrice Baudouin ◽  
Pierre M. Ronco
Keyword(s):  
Cell Line ◽  
Natriuretic Peptides ◽  
Collecting Duct ◽  
Duct Cell ◽  
Cortical Collecting Duct

scholarly journals Activation of the Amiloride-Sensitive Epithelial Sodium Channel by the Serine Protease mCAP1 Expressed in a Mouse Cortical Collecting Duct Cell Line

2000 ◽  
Vol 11 (5) ◽  
pp. 828-834
Author(s):  
GRÉGOIRE VUAGNIAUX ◽  
VÉRONIQUE VALLET ◽  
NICOLE FOWLER JAEGER ◽  
CORINNE PFISTER ◽  
MARCELLE BENS ◽  
...  
Keyword(s):  
Sodium Channel ◽  
Cell Line ◽  
Serine Protease ◽  
Sodium Transport ◽  
Serine Proteases ◽  
Collecting Duct ◽  
Short Circuit ◽  
Short Circuit Current ◽  
Duct Cell ◽  
Cortical Collecting Duct

Abstract. This study examines whether serine proteases can activate the amiloride-sensitive sodium channel (ENaC) in mammalian kidney epithelial cells. The transepithelial sodium transport assessed by amiloride-sensitive short-circuit current appears to be sensitive to aprotinin, a protease inhibitor in a mouse cortical collecting duct cell line (mpkCCDc14). This result indicated that serine proteases may be implicated in the regulation of ENaC-mediated sodium transport. Using degenerated oligonucleotides to a previously isolated serine protease from Xenopus, xCAP1 (channel activating protease), a novel full-length serine protease (mCAP1), has been isolated and characterized. RNA analysis showed a broad pattern of expression in tissues (kidney, lung, colon, and salivary glands) expressing ENaC. Reverse transcription-PCR experiments also showed that mCAP1 was abundantly expressed in proximal tubule cells and was also expressed in intact and cultured collecting duct cells. Coexpression of the Xenopus, rat, or human α-, β-, and γ-ENaC subunits in Xenopus oocytes also showed that mCAP1 induces a significant increase in ENaC-mediated current accompanied by a decrease of channel molecules at the cell surface. It is proposed that this novel mouse channel activating protease may act as a regulator of ENaC within the kidney.

scholarly journals Characteristics of a rat cortical collecting duct cell line that maintains high transepithelial resistance

1996 ◽  
Vol 50 (2) ◽  
pp. 367-376 ◽  
Author(s):  
Marcel Blot-Chabaud ◽  
Monique Laplace ◽  
Françoise Cluzeaud ◽  
Claudia Capurro ◽  
Roland Cassingéna ◽  
...  
Keyword(s):  
Cell Line ◽  
Collecting Duct ◽  
Duct Cell ◽  
Transepithelial Resistance ◽  
Cortical Collecting Duct
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