scholarly journals Effects of chemical chaperones on partially retarded NaCl cotransporter mutants associated with Gitelman's syndrome in a mouse cortical collecting duct cell line

2004 ◽  
Vol 19 (5) ◽  
pp. 1069-1076 ◽  
Author(s):  
J. C. de Jong ◽  
P. H. G. M. Willems ◽  
M. Goossens ◽  
A. Vandewalle ◽  
L. P. W. J. van den Heuvel ◽  
...  
Keyword(s):  
Cell Line ◽  
Collecting Duct ◽  
Duct Cell ◽  
Cortical Collecting Duct ◽  
Chemical Chaperones ◽  
Gitelman's Syndrome ◽  
Nacl Cotransporter

scholarly journals Transcriptome of a mouse kidney cortical collecting duct cell line: Effects of aldosterone and vasopressin

2001 ◽  
Vol 98 (5) ◽  
pp. 2712-2716 ◽  
Author(s):  
M. Robert-Nicoud ◽  
M. Flahaut ◽  
J.-M. Elalouf ◽  
M. Nicod ◽  
M. Salinas ◽  
...  
Keyword(s):  
Cell Line ◽  
Collecting Duct ◽  
Duct Cell ◽  
Mouse Kidney ◽  
Cortical Collecting Duct

Vasopressin Regulates Water Flow in a Rat Cortical Collecting Duct Cell Line Not Containing Known Aquaporins

2001 ◽  
Vol 179 (1) ◽  
pp. 63-70 ◽  
Author(s):  
C. Capurro ◽  
V. Rivarola ◽  
A. Kierbel ◽  
B. Escoubet ◽  
N. Farman ◽  
...  
Keyword(s):  
Cell Line ◽  
Water Flow ◽  
Collecting Duct ◽  
Duct Cell ◽  
Cortical Collecting Duct

The isolated C-terminus of polycystin-1 promotes increased ATP-stimulated chloride secretion in a collecting duct cell line

2003 ◽  
Vol 104 (3) ◽  
pp. 217-221 ◽  
Author(s):  
Kimberly M. HOOPER ◽  
Robert J. UNWIN ◽  
Michael SUTTERS
Keyword(s):  
Fusion Protein ◽  
Cell Line ◽  
Collecting Duct ◽  
Basolateral Membrane ◽  
Duct Cell ◽  
Chloride Secretion ◽  
Cortical Collecting Duct ◽  
Membrane Expression ◽  
Fusion Protein Expression ◽  
C Terminus

Cyst expansion in autosomal dominant polycystic kidney disease (ADPKD) requires accumulation of fluid into the cyst lumen, which is probably driven by aberrant chloride secretion by the cyst lining epithelium. Extracellular ATP is a potent stimulus for chloride secretion in many epithelial systems, and provides a plausible mechanism for secretion in ADPKD. Therefore the link between polycystin-1 and ATP-stimulated chloride secretion was investigated in the M1 cortical collecting duct cell line. M1 cells were stably transfected with a glucocorticoid-inducible cytoplasmic C-terminal polycystin-1 construct fused to a membrane expression cassette. Induction of fusion protein expression was associated with augmentation of ATP-stimulated transepithelial chloride secretion. After nystatin-induced permeabilization of the basolateral membrane, it was determined that expression of the polycystin fusion protein modulated an ATP-responsive apical chloride conductance. It is concluded that up-regulation of ATP-stimulated chloride secretion might play a significant role in cyst expansion in ADPKD.

scholarly journals Receptors for natriuretic peptides in a human cortical collecting duct cell line

1997 ◽  
Vol 51 (1) ◽  
pp. 281-287 ◽  
Author(s):  
Valerie Millul ◽  
Nicole Ardaillou ◽  
Sandrine Placier ◽  
Béatrice Baudouin ◽  
Pierre M. Ronco
Keyword(s):  
Cell Line ◽  
Natriuretic Peptides ◽  
Collecting Duct ◽  
Duct Cell ◽  
Cortical Collecting Duct

scholarly journals Activation of the Amiloride-Sensitive Epithelial Sodium Channel by the Serine Protease mCAP1 Expressed in a Mouse Cortical Collecting Duct Cell Line

2000 ◽  
Vol 11 (5) ◽  
pp. 828-834
Author(s):  
GRÉGOIRE VUAGNIAUX ◽  
VÉRONIQUE VALLET ◽  
NICOLE FOWLER JAEGER ◽  
CORINNE PFISTER ◽  
MARCELLE BENS ◽  
...  
Keyword(s):  
Sodium Channel ◽  
Cell Line ◽  
Serine Protease ◽  
Sodium Transport ◽  
Serine Proteases ◽  
Collecting Duct ◽  
Short Circuit ◽  
Short Circuit Current ◽  
Duct Cell ◽  
Cortical Collecting Duct

Abstract. This study examines whether serine proteases can activate the amiloride-sensitive sodium channel (ENaC) in mammalian kidney epithelial cells. The transepithelial sodium transport assessed by amiloride-sensitive short-circuit current appears to be sensitive to aprotinin, a protease inhibitor in a mouse cortical collecting duct cell line (mpkCCDc14). This result indicated that serine proteases may be implicated in the regulation of ENaC-mediated sodium transport. Using degenerated oligonucleotides to a previously isolated serine protease from Xenopus, xCAP1 (channel activating protease), a novel full-length serine protease (mCAP1), has been isolated and characterized. RNA analysis showed a broad pattern of expression in tissues (kidney, lung, colon, and salivary glands) expressing ENaC. Reverse transcription-PCR experiments also showed that mCAP1 was abundantly expressed in proximal tubule cells and was also expressed in intact and cultured collecting duct cells. Coexpression of the Xenopus, rat, or human α-, β-, and γ-ENaC subunits in Xenopus oocytes also showed that mCAP1 induces a significant increase in ENaC-mediated current accompanied by a decrease of channel molecules at the cell surface. It is proposed that this novel mouse channel activating protease may act as a regulator of ENaC within the kidney.

scholarly journals Characteristics of a rat cortical collecting duct cell line that maintains high transepithelial resistance

1996 ◽  
Vol 50 (2) ◽  
pp. 367-376 ◽  
Author(s):  
Marcel Blot-Chabaud ◽  
Monique Laplace ◽  
Françoise Cluzeaud ◽  
Claudia Capurro ◽  
Roland Cassingéna ◽  
...  
Keyword(s):  
Cell Line ◽  
Collecting Duct ◽  
Duct Cell ◽  
Transepithelial Resistance ◽  
Cortical Collecting Duct

scholarly journals Regulation of vitamin D-1α-hydroxylase in a human cortical collecting duct cell line

2001 ◽  
Vol 60 (4) ◽  
pp. 1277-1286 ◽  
Author(s):  
Rosemary Bland ◽  
Daniel Zehnder ◽  
Susan V. Hughes ◽  
Pierre M. Ronco ◽  
Paul M. Stewart ◽  
...  
Keyword(s):  
Vitamin D ◽  
Cell Line ◽  
Collecting Duct ◽  
Duct Cell ◽  
Cortical Collecting Duct

scholarly journals Role of adenosine on glucagon-induced cAMP in a human cortical collecting duct cell line

1995 ◽  
Vol 47 (5) ◽  
pp. 1310-1318 ◽  
Author(s):  
Dominique Prié ◽  
Gérard Friedlander ◽  
Christiane Coureau ◽  
Alain Vandewalle ◽  
Roland Cassingéna ◽  
...  
Keyword(s):  
Cell Line ◽  
Collecting Duct ◽  
Duct Cell ◽  
Cortical Collecting Duct
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